Abstract
| Original language | English |
|---|---|
| Qualification | Doctor of Philosophy |
| Awarding Institution |
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| Supervisors/Advisors |
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| Thesis sponsors | |
| Award date | 30 Aug 2013 |
| Place of Publication | Zutphen |
| Publisher | |
| Print ISBNs | 978-90-365-0263-4 |
| DOIs | |
| Publication status | Published - 30 Aug 2013 |
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Keywords
- EWI-23681
- IR-86969
- METIS-297242
Cite this
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Electronic DNA detection and diagnostics. / De, Arpita.
Zutphen : Wohrmann Print Service, 2013. 155 p.Research output: Thesis › PhD Thesis - Research UT, graduation UT
TY - THES
T1 - Electronic DNA detection and diagnostics
AU - De, Arpita
PY - 2013/8/30
Y1 - 2013/8/30
N2 - The Nanopill project is an ambitious undertaking with the objective to develop an early-warning cancer diagnostic pill that is ingested by the patient. The Nanopill collects intestinal fluid as the pill travels down the intestinal tract, and tests for the presence of a free floating hyper-methylated DNA (hm-DNA) with a gene sequence specific for colorectal cancer. Since the development of the entire Nanopill system is beyond the scope of a single doctoral thesis, this thesis focused on the development and realization of an automated microscale bioassay for the capture and enrichment of the hm-DNA colorectal cancer marker and the subsequent detection of the specific DNA sequence using all-electronic silicon nanowire (Si-NW) biosensors without the use of amplification. Free-floating hm-DNA is present in very low concentrations in biological samples, such as stool, sputum, urine, blood, and gastrointestinal fluid. A capture and pre-concentraion of hm-DNA would be used, prior to detection on Si-NWs. The pre-concentration system has been designed and implemented using a pillar-based glass-silicon microfluidic chip for the solid-phase extraction of hm-DNA with methyl binding domain (MBD) protein that specifically captures double-stranded hm-DNA in the CpG island hyper-methylation context. The MBD extraction microchip is designed for the capture and elution of hm-DNA with quantities less than 1 ng ml-1 and was able to demonstrate 28× concentration factor of the input concentration.
AB - The Nanopill project is an ambitious undertaking with the objective to develop an early-warning cancer diagnostic pill that is ingested by the patient. The Nanopill collects intestinal fluid as the pill travels down the intestinal tract, and tests for the presence of a free floating hyper-methylated DNA (hm-DNA) with a gene sequence specific for colorectal cancer. Since the development of the entire Nanopill system is beyond the scope of a single doctoral thesis, this thesis focused on the development and realization of an automated microscale bioassay for the capture and enrichment of the hm-DNA colorectal cancer marker and the subsequent detection of the specific DNA sequence using all-electronic silicon nanowire (Si-NW) biosensors without the use of amplification. Free-floating hm-DNA is present in very low concentrations in biological samples, such as stool, sputum, urine, blood, and gastrointestinal fluid. A capture and pre-concentraion of hm-DNA would be used, prior to detection on Si-NWs. The pre-concentration system has been designed and implemented using a pillar-based glass-silicon microfluidic chip for the solid-phase extraction of hm-DNA with methyl binding domain (MBD) protein that specifically captures double-stranded hm-DNA in the CpG island hyper-methylation context. The MBD extraction microchip is designed for the capture and elution of hm-DNA with quantities less than 1 ng ml-1 and was able to demonstrate 28× concentration factor of the input concentration.
KW - EWI-23681
KW - IR-86969
KW - METIS-297242
U2 - 10.3990/1.9789036502634
DO - 10.3990/1.9789036502634
M3 - PhD Thesis - Research UT, graduation UT
SN - 978-90-365-0263-4
PB - Wohrmann Print Service
CY - Zutphen
ER -