Electrospinning of collagen and elastin for tissue engineering applications

L. Buttafoco, N.G. Kolkman, P. Engbers-Buijtenhuijs, Andreas A. Poot, Pieter J. Dijkstra, I. Vermes, Jan Feijen

Research output: Contribution to journalArticleAcademicpeer-review

458 Citations (Scopus)

Abstract

Meshes of collagen and/or elastin were successfully prepared by means of electrospinning from aqueous solutions. Flow rate, applied electric field, collecting distance and composition of the starting solutions determined the morphology of the obtained fibres. Addition of PEO (Mw=8×106) and NaCl was always necessary to spin continuous and homogeneous fibres. Spinning a mixture of collagen and elastin resulted in fibres in which the single components could not be distinguished by SEM. Increasing the elastin content determined an increase in fibres diameters from 220 to 600 nm. The voltage necessary for a continuous production of fibres was dependent on the composition of the starting solution, but always between 10 and 25 kV. Under these conditions, non-woven meshes could be formed and a partial orientation of the fibres constituting the mesh was obtained by using a rotating tubular mandrel as collector. Collagen/elastin (1:1) meshes were stabilized by crosslinking with N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). This treatment afforded materials with a high thermal stability (Td = 79°C) without altering their original morphology. Upon crosslinking PEO and NaCl were fully leached out. Smooth muscle cells grew as a confluent layer on top of the crosslinked meshes after 14 d of culture.
Original languageUndefined
Pages (from-to)724-734
JournalBiomaterials
Volume27
Issue number5
DOIs
Publication statusPublished - 2006

Keywords

  • IR-67199
  • Cross-linking
  • Collagen
  • Elastin
  • Vascular grafts
  • METIS-229769

Cite this

Buttafoco, L. ; Kolkman, N.G. ; Engbers-Buijtenhuijs, P. ; Poot, Andreas A. ; Dijkstra, Pieter J. ; Vermes, I. ; Feijen, Jan. / Electrospinning of collagen and elastin for tissue engineering applications. In: Biomaterials. 2006 ; Vol. 27, No. 5. pp. 724-734.
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Electrospinning of collagen and elastin for tissue engineering applications. / Buttafoco, L.; Kolkman, N.G.; Engbers-Buijtenhuijs, P.; Poot, Andreas A.; Dijkstra, Pieter J.; Vermes, I.; Feijen, Jan.

In: Biomaterials, Vol. 27, No. 5, 2006, p. 724-734.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Electrospinning of collagen and elastin for tissue engineering applications

AU - Buttafoco, L.

AU - Kolkman, N.G.

AU - Engbers-Buijtenhuijs, P.

AU - Poot, Andreas A.

AU - Dijkstra, Pieter J.

AU - Vermes, I.

AU - Feijen, Jan

PY - 2006

Y1 - 2006

N2 - Meshes of collagen and/or elastin were successfully prepared by means of electrospinning from aqueous solutions. Flow rate, applied electric field, collecting distance and composition of the starting solutions determined the morphology of the obtained fibres. Addition of PEO (Mw=8×106) and NaCl was always necessary to spin continuous and homogeneous fibres. Spinning a mixture of collagen and elastin resulted in fibres in which the single components could not be distinguished by SEM. Increasing the elastin content determined an increase in fibres diameters from 220 to 600 nm. The voltage necessary for a continuous production of fibres was dependent on the composition of the starting solution, but always between 10 and 25 kV. Under these conditions, non-woven meshes could be formed and a partial orientation of the fibres constituting the mesh was obtained by using a rotating tubular mandrel as collector. Collagen/elastin (1:1) meshes were stabilized by crosslinking with N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). This treatment afforded materials with a high thermal stability (Td = 79°C) without altering their original morphology. Upon crosslinking PEO and NaCl were fully leached out. Smooth muscle cells grew as a confluent layer on top of the crosslinked meshes after 14 d of culture.

AB - Meshes of collagen and/or elastin were successfully prepared by means of electrospinning from aqueous solutions. Flow rate, applied electric field, collecting distance and composition of the starting solutions determined the morphology of the obtained fibres. Addition of PEO (Mw=8×106) and NaCl was always necessary to spin continuous and homogeneous fibres. Spinning a mixture of collagen and elastin resulted in fibres in which the single components could not be distinguished by SEM. Increasing the elastin content determined an increase in fibres diameters from 220 to 600 nm. The voltage necessary for a continuous production of fibres was dependent on the composition of the starting solution, but always between 10 and 25 kV. Under these conditions, non-woven meshes could be formed and a partial orientation of the fibres constituting the mesh was obtained by using a rotating tubular mandrel as collector. Collagen/elastin (1:1) meshes were stabilized by crosslinking with N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). This treatment afforded materials with a high thermal stability (Td = 79°C) without altering their original morphology. Upon crosslinking PEO and NaCl were fully leached out. Smooth muscle cells grew as a confluent layer on top of the crosslinked meshes after 14 d of culture.

KW - IR-67199

KW - Cross-linking

KW - Collagen

KW - Elastin

KW - Vascular grafts

KW - METIS-229769

U2 - 10.1016/j.biomaterials.2005.06.024

DO - 10.1016/j.biomaterials.2005.06.024

M3 - Article

VL - 27

SP - 724

EP - 734

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 5

ER -