Elevated cytosolic concentrations of SecA compensate for a protein translocation defect in Escherichia coli cells with reduced levels of negatively charged phospholipids

Ron Kusters*, Richard Huijbregts, Ben de Kruijff

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)
100 Downloads (Pure)

Abstract

Cellular extracts from cells with reduced synthesis of negatively charged phospholipids were found to support in vitro translocation of the precursor of the outer membrane protein PhoE with increased efficiency. Analysis of these extracts revealed that they contain increased levels of SecA. SecA depletion resulted in a loss of the translocation stimulatory activity, which could be restored by re-addition of purified SecA. We conclude that elevated cytosolic levels of SecA counteract the reduction of translocation efficiency due to low levels of negatively charged phospholipids in the inner membrane.

Original languageEnglish
Pages (from-to)97-100
Number of pages4
JournalFEBS letters
Volume308
Issue number1
DOIs
Publication statusPublished - 10 Aug 1992
Externally publishedYes

Keywords

  • Bacterial membrane
  • PhoE
  • Protein translocation: SecA

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