Elimination of adsorptive behaviour of biomolecules at the glass-solution interface in fluorescence correlation spectroscopy

D. Zhang; R. Vermeij; A. Lenferink; I. Segers-Nolten; C. Blum; V. Subramaniam; C. Otto

doi:10.1007/s00249-007-0178-7

Elimination of adsorptive behaviour of biomolecules at the glass-solution interface in fluorescence correlation spectroscopy

D. Zhang, R. Vermeij, A. Lenferink, I. Segers-Nolten, C. Blum, V. Subramaniam, C. Otto

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Abstract

We study adsorptive behaviour of biomolecules at the glass-solution interface in fluorescence correlation spectroscopy (FCS), and propose a negatively charged coating to eliminate the adsorption of molecules. In this article, we demonstrate confocal microscopic measurements on Cy3.5-90-mer-ssDNA and Cy3.5-90-bp-dsDNA in different solutions, and use two polymers – poly (acrylic acid, sodium salt) and poly (sodium 4-styrenesulfonate) to produce the negatively charged coating on glass coverslips. This technology enables more stable FCS measurements in extremely low concentration samples and reveals that the adsorptive behaviour of biomolecules is responsible for sudden disappearance of many iomolecules in low concentration solutions.

Original language	English
Article number	P-503
Pages (from-to)	S181-S181
Journal	European biophysics journal
Volume	36
Issue number	1
DOIs	https://doi.org/10.1007/s00249-007-0178-7
Publication status	Published - 2007
Event	6th European Biophysics Congress, EBSA 2007 - London, United Kingdom Duration: 14 Jul 2007 → 19 Jul 2007 Conference number: 6

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title = "Elimination of adsorptive behaviour of biomolecules at the glass-solution interface in fluorescence correlation spectroscopy",

abstract = "We study adsorptive behaviour of biomolecules at the glass-solution interface in fluorescence correlation spectroscopy (FCS), and propose a negatively charged coating to eliminate the adsorption of molecules. In this article, we demonstrate confocal microscopic measurements on Cy3.5-90-mer-ssDNA and Cy3.5-90-bp-dsDNA in different solutions, and use two polymers – poly (acrylic acid, sodium salt) and poly (sodium 4-styrenesulfonate) to produce the negatively charged coating on glass coverslips. This technology enables more stable FCS measurements in extremely low concentration samples and reveals that the adsorptive behaviour of biomolecules is responsible for sudden disappearance of many iomolecules in low concentration solutions.",

author = "D. Zhang and R. Vermeij and A. Lenferink and I. Segers-Nolten and C. Blum and V. Subramaniam and C. Otto",

year = "2007",

doi = "10.1007/s00249-007-0178-7",

language = "English",

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pages = "S181--S181",

journal = "European biophysics journal",

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note = "6th European Biophysics Congress, EBSA 2007, EBSA ; Conference date: 14-07-2007 Through 19-07-2007",

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TY - JOUR

T1 - Elimination of adsorptive behaviour of biomolecules at the glass-solution interface in fluorescence correlation spectroscopy

AU - Zhang, D.

AU - Vermeij, R.

AU - Lenferink, A.

AU - Segers-Nolten, I.

AU - Blum, C.

AU - Subramaniam, V.

AU - Otto, C.

N1 - Conference code: 6

PY - 2007

Y1 - 2007

N2 - We study adsorptive behaviour of biomolecules at the glass-solution interface in fluorescence correlation spectroscopy (FCS), and propose a negatively charged coating to eliminate the adsorption of molecules. In this article, we demonstrate confocal microscopic measurements on Cy3.5-90-mer-ssDNA and Cy3.5-90-bp-dsDNA in different solutions, and use two polymers – poly (acrylic acid, sodium salt) and poly (sodium 4-styrenesulfonate) to produce the negatively charged coating on glass coverslips. This technology enables more stable FCS measurements in extremely low concentration samples and reveals that the adsorptive behaviour of biomolecules is responsible for sudden disappearance of many iomolecules in low concentration solutions.

AB - We study adsorptive behaviour of biomolecules at the glass-solution interface in fluorescence correlation spectroscopy (FCS), and propose a negatively charged coating to eliminate the adsorption of molecules. In this article, we demonstrate confocal microscopic measurements on Cy3.5-90-mer-ssDNA and Cy3.5-90-bp-dsDNA in different solutions, and use two polymers – poly (acrylic acid, sodium salt) and poly (sodium 4-styrenesulfonate) to produce the negatively charged coating on glass coverslips. This technology enables more stable FCS measurements in extremely low concentration samples and reveals that the adsorptive behaviour of biomolecules is responsible for sudden disappearance of many iomolecules in low concentration solutions.

U2 - 10.1007/s00249-007-0178-7

DO - 10.1007/s00249-007-0178-7

M3 - Meeting Abstract

SN - 0175-7571

VL - 36

SP - S181-S181

JO - European biophysics journal

JF - European biophysics journal

IS - 1

M1 - P-503

T2 - 6th European Biophysics Congress, EBSA 2007

Y2 - 14 July 2007 through 19 July 2007

ER -

Elimination of adsorptive behaviour of biomolecules at the glass-solution interface in fluorescence correlation spectroscopy

Abstract

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