Elimination of adsorptive behaviour of biomolecules at the glass-solution interface in fluorescence correlation spectroscopy

D. Zhang, R. Vermeij, A. Lenferink, I. Segers-Nolten, C. Blum, V. Subramaniam, C. Otto

    Research output: Contribution to journalMeeting AbstractAcademic

    Abstract

    We study adsorptive behaviour of biomolecules at the glass-solution interface in fluorescence correlation spectroscopy (FCS), and propose a negatively charged coating to eliminate the adsorption of molecules. In this article, we demonstrate confocal microscopic measurements on Cy3.5-90-mer-ssDNA and Cy3.5-90-bp-dsDNA in different solutions, and use two polymers – poly (acrylic acid, sodium salt) and poly (sodium 4-styrenesulfonate) to produce the negatively charged coating on glass coverslips. This technology enables more stable FCS measurements in extremely low concentration samples and reveals that the adsorptive behaviour of biomolecules is responsible for sudden disappearance of many iomolecules in low concentration solutions.
    Original languageEnglish
    Article numberP-503
    Pages (from-to)S181-S181
    JournalEuropean biophysics journal
    Volume36
    Issue number1
    DOIs
    Publication statusPublished - 2007
    Event6th European Biophysics Congress, EBSA 2007 - London, United Kingdom
    Duration: 14 Jul 200719 Jul 2007
    Conference number: 6

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