e‑MALDI: An Electrowetting-Enhanced Drop Drying Method for MALDI Mass Spectrometry

Olena Kudina, Burak Eral, Friedrich Gunther Mugele

Research output: Contribution to journalArticleAcademicpeer-review

38 Citations (Scopus)

Abstract

The performance of matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is frequently compromised by the heterogeneous distribution of matrix and analyte deposits on the target plate arising during the conventional drop-drying sample preparation procedure. It was recently shown that this so-called coffee stain effect can be suppressed by exciting evaporating complex fluids throughout the drying process using AC-electrowetting. Here, we demonstrate that electrowetting-assisted drying of solutions of common MALDI matrix materials and a variety of common low molecular weight pharmaceutical molecules indeed leads to substantially smaller and more homogeneous sample spots on special electrowetting-functionalized e-MALDI target plates. The improved spot quality enables 2–30× enhanced MALDI-MS signals along with substantial reductions of the typical lateral variations of the MALDI-MS. The latter largely eliminates the time-consuming need to search for “sweet spots”.
Original languageEnglish
Pages (from-to)4669-4675
JournalAnalytical chemistry
Volume88
Issue number9
DOIs
Publication statusPublished - 2016

Keywords

  • IR-102479
  • METIS-319521

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