Endothelial and beta cell composite aggregates for improved function of a bioartificial pancreas encapsulation device

Katarzyna Skrzypek, Yazmin Brito Barrera, Thomas Groth, Dimitrios Stamatialis

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)
8 Downloads (Pure)

Abstract

Introduction: Encapsulation of pancreatic islets or beta cells is a promising strategy for treatment of type 1 diabetes by providing an immune isolated environment and allowing for transplantation in a different location than the liver. However, islets used for encapsulation often show lower functionality due to the damaging of islet endothelial cells during the isolation procedure. Factors produced by endothelial cells have great impact on beta cell insulin secretion. Therefore, mutual signaling between endothelial cells and beta cells should be considered for the development of encapsulation systems to achieve high insulin secretion and maintain beta cell viability. Here, we investigate whether co-culture of beta cells with endothelial cells could improve beta cell function within encapsulation devices. Materials and methods: Mouse insulinoma MIN6 cells and human umbilical vein endothelial cells were used for creating composite aggregates on agarose microwell platform. The composite aggregates were encapsulated within flat poly(ether sulfone)/polyvinylpyrrolidone device. Their functionality was assessed by glucose-induced insulin secretion test and compared to non-encapsulated free-floating aggregates. Results: We created composite aggregates of 80–100 µm in diameter, closely mimicking pancreatic islets. Upon glucose stimulation, their insulin secretion is improved in comparison to aggregates consisting of only MIN6 cells. Moreover, the composite aggregates encapsulated within a device secrete more insulin than aggregates consisting of only MIN6 cells. Conclusion: Composite aggregates of MIN6 cells with human umbilical vein endothelial cells have improved insulin secretion in comparison to MIN6 aggregates showing that the interaction of beta cell and endothelial cell is crucial for a functional encapsulation system.

Original languageEnglish
Pages (from-to)152-159
JournalInternational journal of artificial organs
Volume41
Issue number3
Early online date20 Feb 2018
DOIs
Publication statusPublished - 1 Mar 2018

Fingerprint

Encapsulation
Endothelial cells
Pancreas
Endothelial Cells
Insulin
Equipment and Supplies
Composite materials
Islets of Langerhans
Human Umbilical Vein Endothelial Cells
Glucose
Povidone
Insulinoma
Sulfones
Cell Separation
Medical problems
Coculture Techniques
Type 1 Diabetes Mellitus
Cell culture
Cell Communication
Liver

Keywords

  • beta cells
  • Bioartificial pancreas
  • composite aggregates
  • encapsulation
  • human umbilical vein endothelial cell

Cite this

@article{2a8183f9aa7d4bdb91b5395a90757eb4,
title = "Endothelial and beta cell composite aggregates for improved function of a bioartificial pancreas encapsulation device",
abstract = "Introduction: Encapsulation of pancreatic islets or beta cells is a promising strategy for treatment of type 1 diabetes by providing an immune isolated environment and allowing for transplantation in a different location than the liver. However, islets used for encapsulation often show lower functionality due to the damaging of islet endothelial cells during the isolation procedure. Factors produced by endothelial cells have great impact on beta cell insulin secretion. Therefore, mutual signaling between endothelial cells and beta cells should be considered for the development of encapsulation systems to achieve high insulin secretion and maintain beta cell viability. Here, we investigate whether co-culture of beta cells with endothelial cells could improve beta cell function within encapsulation devices. Materials and methods: Mouse insulinoma MIN6 cells and human umbilical vein endothelial cells were used for creating composite aggregates on agarose microwell platform. The composite aggregates were encapsulated within flat poly(ether sulfone)/polyvinylpyrrolidone device. Their functionality was assessed by glucose-induced insulin secretion test and compared to non-encapsulated free-floating aggregates. Results: We created composite aggregates of 80–100 µm in diameter, closely mimicking pancreatic islets. Upon glucose stimulation, their insulin secretion is improved in comparison to aggregates consisting of only MIN6 cells. Moreover, the composite aggregates encapsulated within a device secrete more insulin than aggregates consisting of only MIN6 cells. Conclusion: Composite aggregates of MIN6 cells with human umbilical vein endothelial cells have improved insulin secretion in comparison to MIN6 aggregates showing that the interaction of beta cell and endothelial cell is crucial for a functional encapsulation system.",
keywords = "beta cells, Bioartificial pancreas, composite aggregates, encapsulation, human umbilical vein endothelial cell",
author = "Katarzyna Skrzypek and Barrera, {Yazmin Brito} and Thomas Groth and Dimitrios Stamatialis",
year = "2018",
month = "3",
day = "1",
doi = "10.1177/0391398817752295",
language = "English",
volume = "41",
pages = "152--159",
journal = "International journal of artificial organs",
issn = "0391-3988",
publisher = "Wichtig Publishing",
number = "3",

}

Endothelial and beta cell composite aggregates for improved function of a bioartificial pancreas encapsulation device. / Skrzypek, Katarzyna; Barrera, Yazmin Brito; Groth, Thomas; Stamatialis, Dimitrios.

In: International journal of artificial organs, Vol. 41, No. 3, 01.03.2018, p. 152-159.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Endothelial and beta cell composite aggregates for improved function of a bioartificial pancreas encapsulation device

AU - Skrzypek, Katarzyna

AU - Barrera, Yazmin Brito

AU - Groth, Thomas

AU - Stamatialis, Dimitrios

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Introduction: Encapsulation of pancreatic islets or beta cells is a promising strategy for treatment of type 1 diabetes by providing an immune isolated environment and allowing for transplantation in a different location than the liver. However, islets used for encapsulation often show lower functionality due to the damaging of islet endothelial cells during the isolation procedure. Factors produced by endothelial cells have great impact on beta cell insulin secretion. Therefore, mutual signaling between endothelial cells and beta cells should be considered for the development of encapsulation systems to achieve high insulin secretion and maintain beta cell viability. Here, we investigate whether co-culture of beta cells with endothelial cells could improve beta cell function within encapsulation devices. Materials and methods: Mouse insulinoma MIN6 cells and human umbilical vein endothelial cells were used for creating composite aggregates on agarose microwell platform. The composite aggregates were encapsulated within flat poly(ether sulfone)/polyvinylpyrrolidone device. Their functionality was assessed by glucose-induced insulin secretion test and compared to non-encapsulated free-floating aggregates. Results: We created composite aggregates of 80–100 µm in diameter, closely mimicking pancreatic islets. Upon glucose stimulation, their insulin secretion is improved in comparison to aggregates consisting of only MIN6 cells. Moreover, the composite aggregates encapsulated within a device secrete more insulin than aggregates consisting of only MIN6 cells. Conclusion: Composite aggregates of MIN6 cells with human umbilical vein endothelial cells have improved insulin secretion in comparison to MIN6 aggregates showing that the interaction of beta cell and endothelial cell is crucial for a functional encapsulation system.

AB - Introduction: Encapsulation of pancreatic islets or beta cells is a promising strategy for treatment of type 1 diabetes by providing an immune isolated environment and allowing for transplantation in a different location than the liver. However, islets used for encapsulation often show lower functionality due to the damaging of islet endothelial cells during the isolation procedure. Factors produced by endothelial cells have great impact on beta cell insulin secretion. Therefore, mutual signaling between endothelial cells and beta cells should be considered for the development of encapsulation systems to achieve high insulin secretion and maintain beta cell viability. Here, we investigate whether co-culture of beta cells with endothelial cells could improve beta cell function within encapsulation devices. Materials and methods: Mouse insulinoma MIN6 cells and human umbilical vein endothelial cells were used for creating composite aggregates on agarose microwell platform. The composite aggregates were encapsulated within flat poly(ether sulfone)/polyvinylpyrrolidone device. Their functionality was assessed by glucose-induced insulin secretion test and compared to non-encapsulated free-floating aggregates. Results: We created composite aggregates of 80–100 µm in diameter, closely mimicking pancreatic islets. Upon glucose stimulation, their insulin secretion is improved in comparison to aggregates consisting of only MIN6 cells. Moreover, the composite aggregates encapsulated within a device secrete more insulin than aggregates consisting of only MIN6 cells. Conclusion: Composite aggregates of MIN6 cells with human umbilical vein endothelial cells have improved insulin secretion in comparison to MIN6 aggregates showing that the interaction of beta cell and endothelial cell is crucial for a functional encapsulation system.

KW - beta cells

KW - Bioartificial pancreas

KW - composite aggregates

KW - encapsulation

KW - human umbilical vein endothelial cell

UR - http://www.scopus.com/inward/record.url?scp=85045107131&partnerID=8YFLogxK

U2 - 10.1177/0391398817752295

DO - 10.1177/0391398817752295

M3 - Article

VL - 41

SP - 152

EP - 159

JO - International journal of artificial organs

JF - International journal of artificial organs

SN - 0391-3988

IS - 3

ER -