TY - JOUR
T1 - Endothelial cell spreading on lipid bilayers with combined integrin and cadherin binding ligands
AU - Koçer, Gülistan
AU - Albino, Inês M.C.
AU - Verheijden, Mark L.
AU - Jonkheijm, Pascal
N1 - Funding Information:
The authors acknowledge the Netherlands Organization for Scientific Research (NWO) (NWO-VIDI 723.012.106) for financial support. We thank Prof. Ralf Richter for SE measurements.
Publisher Copyright:
© 2022 The Authors
PY - 2022/8/15
Y1 - 2022/8/15
N2 - Endothelial cells play a central role in the vascular system, where their function is tightly regulated by both cell-extracellular matrix (e.g., via integrins) and cell–cell interactions (e.g., via cadherins). In this study, we incorporated cholesterol-modified integrin and N-cadherin peptide binding ligands in fluid supported lipid bilayers. Human umbilical vein endothelial cell adhesion, spreading and vinculin localization in these cells were dependent on ligand density. One composition led to observe a higher extent of cell spreading, where cells exhibited extensive lamellipodia formation and a qualitatively more distinct N-cadherin localization at the cell periphery, which is indicative of N-cadherin clustering and a mimic of cell–cell contact formation. The results can be used to reconstitute the endothelial-pericyte interface on biomedical devices and materials.
AB - Endothelial cells play a central role in the vascular system, where their function is tightly regulated by both cell-extracellular matrix (e.g., via integrins) and cell–cell interactions (e.g., via cadherins). In this study, we incorporated cholesterol-modified integrin and N-cadherin peptide binding ligands in fluid supported lipid bilayers. Human umbilical vein endothelial cell adhesion, spreading and vinculin localization in these cells were dependent on ligand density. One composition led to observe a higher extent of cell spreading, where cells exhibited extensive lamellipodia formation and a qualitatively more distinct N-cadherin localization at the cell periphery, which is indicative of N-cadherin clustering and a mimic of cell–cell contact formation. The results can be used to reconstitute the endothelial-pericyte interface on biomedical devices and materials.
KW - Cell-instructive
KW - Cell-surface interactions
KW - Peptide ligands
KW - Supported lipid bilayers
KW - Surface modification
KW - UT-Hybrid-D
UR - http://www.scopus.com/inward/record.url?scp=85133103279&partnerID=8YFLogxK
U2 - 10.1016/j.bmc.2022.116850
DO - 10.1016/j.bmc.2022.116850
M3 - Article
C2 - 35714536
AN - SCOPUS:85133103279
VL - 68
JO - Bioorganic & medicinal chemistry
JF - Bioorganic & medicinal chemistry
SN - 0968-0896
M1 - 116850
ER -