Endothelial Cells in Peripheral Blood of Healthy Subjects and Patients with Metastatic Carcinomas

Jason L. Rowand, Grace Martin, Gerald V. Doyle, M. Craig Miller, Michael S. Pierce, Mark C. Connelly, Chandra Rao, L.W.M.M. Terstappen

    Research output: Contribution to journalArticleAcademicpeer-review

    106 Citations (Scopus)

    Abstract

    BACKGROUND: A lack of standardized assays and consensus of cell definition has lead to a wide variation in the reported range of circulating endothelial cells (CECs). METHODS: An automated rare cell analysis system was used to enumerate nucleated, CD146+/CD105+/CD45- CECs in 4 mL of blood. RESULTS: Recoveries of spiked HUVECs were linear over a range of 0-1,241 cells (R2>or=0.99) with recoveries of >or=70% at each spike level. Correlation coefficient values for interoperator variability and duplicate sample variation were (R2=0.99 and 0.90), respectively. Correlation of CEC counts between tubes 1-2 and 2-3 drawn from the same subject in sequence differed (R2=0.48 and 0.63, respectively). The normal CEC reference range established in 249 healthy donors was 1-20 CECs/mL blood. CEC counts were significantly higher in the 206 metastatic carcinoma patients (P<0.0001). CONCLUSION: CECs can be accurately and reproducibly enumerated in blood and are elevated in metastatic carcinomas compared with healthy donors. Phlebotomy procedures can affect endothelial cell counts.
    Original languageEnglish
    Pages (from-to)105-113
    Number of pages9
    JournalCytometry. Part A
    VolumePart A 71A
    DOIs
    Publication statusPublished - 2007

    Keywords

    • METIS-242655

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