Endothelial Cells in Peripheral Blood of Healthy Subjects and Patients with Metastatic Carcinomas

Jason L. Rowand; Grace Martin; Gerald V. Doyle; M. Craig Miller; Michael S. Pierce; Mark C. Connelly; Chandra Rao; L.W.M.M. Terstappen

doi:10.1002/cyto.a.20364

Endothelial Cells in Peripheral Blood of Healthy Subjects and Patients with Metastatic Carcinomas

Jason L. Rowand, Grace Martin, Gerald V. Doyle, M. Craig Miller, Michael S. Pierce, Mark C. Connelly, Chandra Rao, L.W.M.M. Terstappen

Research output: Contribution to journal › Article › Academic › peer-review

115 Citations (Scopus)

3 Downloads (Pure)

Abstract

BACKGROUND: A lack of standardized assays and consensus of cell definition has lead to a wide variation in the reported range of circulating endothelial cells (CECs). METHODS: An automated rare cell analysis system was used to enumerate nucleated, CD146+/CD105+/CD45- CECs in 4 mL of blood. RESULTS: Recoveries of spiked HUVECs were linear over a range of 0-1,241 cells (R2>or=0.99) with recoveries of >or=70% at each spike level. Correlation coefficient values for interoperator variability and duplicate sample variation were (R2=0.99 and 0.90), respectively. Correlation of CEC counts between tubes 1-2 and 2-3 drawn from the same subject in sequence differed (R2=0.48 and 0.63, respectively). The normal CEC reference range established in 249 healthy donors was 1-20 CECs/mL blood. CEC counts were significantly higher in the 206 metastatic carcinoma patients (P<0.0001). CONCLUSION: CECs can be accurately and reproducibly enumerated in blood and are elevated in metastatic carcinomas compared with healthy donors. Phlebotomy procedures can affect endothelial cell counts.

Original language	English
Pages (from-to)	105-113
Number of pages	9
Journal	Cytometry. Part A
Volume	Part A 71A
DOIs	https://doi.org/10.1002/cyto.a.20364
Publication status	Published - 2007

Keywords

METIS-242655

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/cyto.a.20364

Cite this

@article{5d797f39bec2470cb93b8e025da1f21e,

title = "Endothelial Cells in Peripheral Blood of Healthy Subjects and Patients with Metastatic Carcinomas",

abstract = "BACKGROUND: A lack of standardized assays and consensus of cell definition has lead to a wide variation in the reported range of circulating endothelial cells (CECs). METHODS: An automated rare cell analysis system was used to enumerate nucleated, CD146+/CD105+/CD45- CECs in 4 mL of blood. RESULTS: Recoveries of spiked HUVECs were linear over a range of 0-1,241 cells (R2>or=0.99) with recoveries of >or=70% at each spike level. Correlation coefficient values for interoperator variability and duplicate sample variation were (R2=0.99 and 0.90), respectively. Correlation of CEC counts between tubes 1-2 and 2-3 drawn from the same subject in sequence differed (R2=0.48 and 0.63, respectively). The normal CEC reference range established in 249 healthy donors was 1-20 CECs/mL blood. CEC counts were significantly higher in the 206 metastatic carcinoma patients (P<0.0001). CONCLUSION: CECs can be accurately and reproducibly enumerated in blood and are elevated in metastatic carcinomas compared with healthy donors. Phlebotomy procedures can affect endothelial cell counts.",

keywords = "METIS-242655",

author = "Rowand, {Jason L.} and Grace Martin and Doyle, {Gerald V.} and Miller, {M. Craig} and Pierce, {Michael S.} and Connelly, {Mark C.} and Chandra Rao and L.W.M.M. Terstappen",

year = "2007",

doi = "10.1002/cyto.a.20364",

language = "English",

volume = "Part A 71A",

pages = "105--113",

journal = "Cytometry. Part A",

issn = "1552-4922",

publisher = "Wiley",

}

TY - JOUR

T1 - Endothelial Cells in Peripheral Blood of Healthy Subjects and Patients with Metastatic Carcinomas

AU - Rowand, Jason L.

AU - Martin, Grace

AU - Doyle, Gerald V.

AU - Miller, M. Craig

AU - Pierce, Michael S.

AU - Connelly, Mark C.

AU - Rao, Chandra

AU - Terstappen, L.W.M.M.

PY - 2007

Y1 - 2007

N2 - BACKGROUND: A lack of standardized assays and consensus of cell definition has lead to a wide variation in the reported range of circulating endothelial cells (CECs). METHODS: An automated rare cell analysis system was used to enumerate nucleated, CD146+/CD105+/CD45- CECs in 4 mL of blood. RESULTS: Recoveries of spiked HUVECs were linear over a range of 0-1,241 cells (R2>or=0.99) with recoveries of >or=70% at each spike level. Correlation coefficient values for interoperator variability and duplicate sample variation were (R2=0.99 and 0.90), respectively. Correlation of CEC counts between tubes 1-2 and 2-3 drawn from the same subject in sequence differed (R2=0.48 and 0.63, respectively). The normal CEC reference range established in 249 healthy donors was 1-20 CECs/mL blood. CEC counts were significantly higher in the 206 metastatic carcinoma patients (P<0.0001). CONCLUSION: CECs can be accurately and reproducibly enumerated in blood and are elevated in metastatic carcinomas compared with healthy donors. Phlebotomy procedures can affect endothelial cell counts.

AB - BACKGROUND: A lack of standardized assays and consensus of cell definition has lead to a wide variation in the reported range of circulating endothelial cells (CECs). METHODS: An automated rare cell analysis system was used to enumerate nucleated, CD146+/CD105+/CD45- CECs in 4 mL of blood. RESULTS: Recoveries of spiked HUVECs were linear over a range of 0-1,241 cells (R2>or=0.99) with recoveries of >or=70% at each spike level. Correlation coefficient values for interoperator variability and duplicate sample variation were (R2=0.99 and 0.90), respectively. Correlation of CEC counts between tubes 1-2 and 2-3 drawn from the same subject in sequence differed (R2=0.48 and 0.63, respectively). The normal CEC reference range established in 249 healthy donors was 1-20 CECs/mL blood. CEC counts were significantly higher in the 206 metastatic carcinoma patients (P<0.0001). CONCLUSION: CECs can be accurately and reproducibly enumerated in blood and are elevated in metastatic carcinomas compared with healthy donors. Phlebotomy procedures can affect endothelial cell counts.

KW - METIS-242655

U2 - 10.1002/cyto.a.20364

DO - 10.1002/cyto.a.20364

M3 - Article

SN - 1552-4922

VL - Part A 71A

SP - 105

EP - 113

JO - Cytometry. Part A

JF - Cytometry. Part A

ER -

Endothelial Cells in Peripheral Blood of Healthy Subjects and Patients with Metastatic Carcinomas

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this