Engineered in vitro disease models

Kambez H. Benam*, Stephanie Dauth, Bryan Hassell, Anna Herland, Abhishek Jain, Kyung Jin Jang, Katia Karalis, Hyun Jung Kim, Luke MacQueen, Roza Mahmoodian, Samira Musah, Yu Suke Torisawa, Andries D. Van Der Meer, Remi Villenave, Moran Yadid, Kevin K. Parker, Donald E. Ingber

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

208 Citations (Scopus)

Abstract

The ultimate goal of most biomedical research is to gain greater insight into mechanisms of human disease or to develop new and improved therapies or diagnostics. Although great advances have been made in terms of developing disease models in animals, such as transgenic mice, many of these models fail to faithfully recapitulate the human condition. In addition, it is difficult to identify critical cellular and molecular contributors to disease or to vary them independently in whole-animal models. This challenge has attracted the interest of engineers, who have begun to collaborate with biologists to leverage recent advances in tissue engineering and microfabrication to develop novel in vitro models of disease. As these models are synthetic systems, specific molecular factors and individual cell types, including parenchymal cells, vascular cells, and immune cells, can be varied independently while simultaneously measuring system-level responses in real time. In this article, we provide some examples of these efforts, including engineered models of diseases of the heart, lung, intestine, liver, kidney, cartilage, skin and vascular, endocrine, musculoskeletal, and nervous systems, as well as models of infectious diseases and cancer. We also describe how engineered in vitro models can be combined with human inducible pluripotent stem cells to enable new insights into a broad variety of disease mechanisms, as well as provide a test bed for screening new therapies.

Original languageEnglish
Pages (from-to)195-262
Number of pages68
JournalAnnual Review of Pathology: Mechanisms of Disease
Volume10
DOIs
Publication statusPublished - 1 Jan 2015
Externally publishedYes

Fingerprint

Blood Vessels
Microtechnology
Animal Disease Models
Musculoskeletal System
Pluripotent Stem Cells
Endocrine System
Tissue Engineering
Nervous System
Transgenic Mice
Lung Diseases
Cartilage
Intestines
Communicable Diseases
Biomedical Research
Heart Diseases
Animal Models
Kidney
Skin
In Vitro Techniques
Liver

Keywords

  • 3D culture
  • disease model
  • in vitro tool
  • microfluidic
  • organ-on-a-chip
  • tissue engineering

Cite this

Benam, K. H., Dauth, S., Hassell, B., Herland, A., Jain, A., Jang, K. J., ... Ingber, D. E. (2015). Engineered in vitro disease models. Annual Review of Pathology: Mechanisms of Disease, 10, 195-262. https://doi.org/10.1146/annurev-pathol-012414-040418
Benam, Kambez H. ; Dauth, Stephanie ; Hassell, Bryan ; Herland, Anna ; Jain, Abhishek ; Jang, Kyung Jin ; Karalis, Katia ; Kim, Hyun Jung ; MacQueen, Luke ; Mahmoodian, Roza ; Musah, Samira ; Torisawa, Yu Suke ; Van Der Meer, Andries D. ; Villenave, Remi ; Yadid, Moran ; Parker, Kevin K. ; Ingber, Donald E. / Engineered in vitro disease models. In: Annual Review of Pathology: Mechanisms of Disease. 2015 ; Vol. 10. pp. 195-262.
@article{cd7456f3259740e2896d985dff9ad957,
title = "Engineered in vitro disease models",
abstract = "The ultimate goal of most biomedical research is to gain greater insight into mechanisms of human disease or to develop new and improved therapies or diagnostics. Although great advances have been made in terms of developing disease models in animals, such as transgenic mice, many of these models fail to faithfully recapitulate the human condition. In addition, it is difficult to identify critical cellular and molecular contributors to disease or to vary them independently in whole-animal models. This challenge has attracted the interest of engineers, who have begun to collaborate with biologists to leverage recent advances in tissue engineering and microfabrication to develop novel in vitro models of disease. As these models are synthetic systems, specific molecular factors and individual cell types, including parenchymal cells, vascular cells, and immune cells, can be varied independently while simultaneously measuring system-level responses in real time. In this article, we provide some examples of these efforts, including engineered models of diseases of the heart, lung, intestine, liver, kidney, cartilage, skin and vascular, endocrine, musculoskeletal, and nervous systems, as well as models of infectious diseases and cancer. We also describe how engineered in vitro models can be combined with human inducible pluripotent stem cells to enable new insights into a broad variety of disease mechanisms, as well as provide a test bed for screening new therapies.",
keywords = "3D culture, disease model, in vitro tool, microfluidic, organ-on-a-chip, tissue engineering",
author = "Benam, {Kambez H.} and Stephanie Dauth and Bryan Hassell and Anna Herland and Abhishek Jain and Jang, {Kyung Jin} and Katia Karalis and Kim, {Hyun Jung} and Luke MacQueen and Roza Mahmoodian and Samira Musah and Torisawa, {Yu Suke} and {Van Der Meer}, {Andries D.} and Remi Villenave and Moran Yadid and Parker, {Kevin K.} and Ingber, {Donald E.}",
year = "2015",
month = "1",
day = "1",
doi = "10.1146/annurev-pathol-012414-040418",
language = "English",
volume = "10",
pages = "195--262",
journal = "Annual Review of Pathology: Mechanisms of Disease",
issn = "1553-4006",
publisher = "Annual Reviews Inc.",

}

Benam, KH, Dauth, S, Hassell, B, Herland, A, Jain, A, Jang, KJ, Karalis, K, Kim, HJ, MacQueen, L, Mahmoodian, R, Musah, S, Torisawa, YS, Van Der Meer, AD, Villenave, R, Yadid, M, Parker, KK & Ingber, DE 2015, 'Engineered in vitro disease models', Annual Review of Pathology: Mechanisms of Disease, vol. 10, pp. 195-262. https://doi.org/10.1146/annurev-pathol-012414-040418

Engineered in vitro disease models. / Benam, Kambez H.; Dauth, Stephanie; Hassell, Bryan; Herland, Anna; Jain, Abhishek; Jang, Kyung Jin; Karalis, Katia; Kim, Hyun Jung; MacQueen, Luke; Mahmoodian, Roza; Musah, Samira; Torisawa, Yu Suke; Van Der Meer, Andries D.; Villenave, Remi; Yadid, Moran; Parker, Kevin K.; Ingber, Donald E.

In: Annual Review of Pathology: Mechanisms of Disease, Vol. 10, 01.01.2015, p. 195-262.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Engineered in vitro disease models

AU - Benam, Kambez H.

AU - Dauth, Stephanie

AU - Hassell, Bryan

AU - Herland, Anna

AU - Jain, Abhishek

AU - Jang, Kyung Jin

AU - Karalis, Katia

AU - Kim, Hyun Jung

AU - MacQueen, Luke

AU - Mahmoodian, Roza

AU - Musah, Samira

AU - Torisawa, Yu Suke

AU - Van Der Meer, Andries D.

AU - Villenave, Remi

AU - Yadid, Moran

AU - Parker, Kevin K.

AU - Ingber, Donald E.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - The ultimate goal of most biomedical research is to gain greater insight into mechanisms of human disease or to develop new and improved therapies or diagnostics. Although great advances have been made in terms of developing disease models in animals, such as transgenic mice, many of these models fail to faithfully recapitulate the human condition. In addition, it is difficult to identify critical cellular and molecular contributors to disease or to vary them independently in whole-animal models. This challenge has attracted the interest of engineers, who have begun to collaborate with biologists to leverage recent advances in tissue engineering and microfabrication to develop novel in vitro models of disease. As these models are synthetic systems, specific molecular factors and individual cell types, including parenchymal cells, vascular cells, and immune cells, can be varied independently while simultaneously measuring system-level responses in real time. In this article, we provide some examples of these efforts, including engineered models of diseases of the heart, lung, intestine, liver, kidney, cartilage, skin and vascular, endocrine, musculoskeletal, and nervous systems, as well as models of infectious diseases and cancer. We also describe how engineered in vitro models can be combined with human inducible pluripotent stem cells to enable new insights into a broad variety of disease mechanisms, as well as provide a test bed for screening new therapies.

AB - The ultimate goal of most biomedical research is to gain greater insight into mechanisms of human disease or to develop new and improved therapies or diagnostics. Although great advances have been made in terms of developing disease models in animals, such as transgenic mice, many of these models fail to faithfully recapitulate the human condition. In addition, it is difficult to identify critical cellular and molecular contributors to disease or to vary them independently in whole-animal models. This challenge has attracted the interest of engineers, who have begun to collaborate with biologists to leverage recent advances in tissue engineering and microfabrication to develop novel in vitro models of disease. As these models are synthetic systems, specific molecular factors and individual cell types, including parenchymal cells, vascular cells, and immune cells, can be varied independently while simultaneously measuring system-level responses in real time. In this article, we provide some examples of these efforts, including engineered models of diseases of the heart, lung, intestine, liver, kidney, cartilage, skin and vascular, endocrine, musculoskeletal, and nervous systems, as well as models of infectious diseases and cancer. We also describe how engineered in vitro models can be combined with human inducible pluripotent stem cells to enable new insights into a broad variety of disease mechanisms, as well as provide a test bed for screening new therapies.

KW - 3D culture

KW - disease model

KW - in vitro tool

KW - microfluidic

KW - organ-on-a-chip

KW - tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=84921913471&partnerID=8YFLogxK

U2 - 10.1146/annurev-pathol-012414-040418

DO - 10.1146/annurev-pathol-012414-040418

M3 - Article

C2 - 25621660

AN - SCOPUS:84921913471

VL - 10

SP - 195

EP - 262

JO - Annual Review of Pathology: Mechanisms of Disease

JF - Annual Review of Pathology: Mechanisms of Disease

SN - 1553-4006

ER -

Benam KH, Dauth S, Hassell B, Herland A, Jain A, Jang KJ et al. Engineered in vitro disease models. Annual Review of Pathology: Mechanisms of Disease. 2015 Jan 1;10:195-262. https://doi.org/10.1146/annurev-pathol-012414-040418