TY - JOUR
T1 - Engineered models of the human heart
T2 - directions and challenges
AU - Stein, Jeroen M.
AU - Mummery, Christine L.
AU - Bellin, Milena
N1 - Funding Information:
This research received support from the Netherlands Organ-on-Chip Initiative, an NWO Gravitation project funded by the Ministry of Education, Culture, and Science of the government of the Netherlands (024.003.001) and the Transnational Research Project on Cardiovascular Diseases (JTC2016_FP-40-021 ACM-HF).
Funding Information:
This research received support from the Netherlands Organ-on-Chip Initiative, an NWO Gravitation project funded by the Ministry of Education, Culture, and Science of the government of the Netherlands ( 024.003.001 ) and the Transnational Research Project on Cardiovascular Diseases ( JTC2016_FP-40-021 ACM-HF ).
Publisher Copyright:
© 2020
PY - 2021/9/14
Y1 - 2021/9/14
N2 - Human heart (patho)physiology is now widely studied using human pluripotent stem cells, but the immaturity of derivative cardiomyocytes has largely limited disease modeling to conditions associated with mutations in cardiac ion channel genes. Recent advances in tissue engineering and organoids have, however, created new opportunities to study diseases beyond “channelopathies.” These synthetic cardiac structures allow quantitative measurement of contraction, force, and other biophysical parameters in three-dimensional configurations, in which the cardiomyocytes in addition become more mature. Multiple cardiac-relevant cell types are also often combined to form organized cardiac tissue mimetic constructs, where cell-cell, cell-extracellular matrix, and paracrine interactions can be mimicked. In this review, we provide an overview of some of the most promising technologies being implemented specifically in personalized heart-on-a-chip models and explore their applications, drawbacks, and potential for future development.
AB - Human heart (patho)physiology is now widely studied using human pluripotent stem cells, but the immaturity of derivative cardiomyocytes has largely limited disease modeling to conditions associated with mutations in cardiac ion channel genes. Recent advances in tissue engineering and organoids have, however, created new opportunities to study diseases beyond “channelopathies.” These synthetic cardiac structures allow quantitative measurement of contraction, force, and other biophysical parameters in three-dimensional configurations, in which the cardiomyocytes in addition become more mature. Multiple cardiac-relevant cell types are also often combined to form organized cardiac tissue mimetic constructs, where cell-cell, cell-extracellular matrix, and paracrine interactions can be mimicked. In this review, we provide an overview of some of the most promising technologies being implemented specifically in personalized heart-on-a-chip models and explore their applications, drawbacks, and potential for future development.
KW - cardiovascular disease modeling
KW - engineered heart tissue
KW - force of contraction
KW - heart-on-a-chip
KW - human pluripotent stem cells
UR - http://www.scopus.com/inward/record.url?scp=85098155677&partnerID=8YFLogxK
U2 - 10.1016/j.stemcr.2020.11.013
DO - 10.1016/j.stemcr.2020.11.013
M3 - Review article
AN - SCOPUS:85098155677
SN - 2213-6711
VL - 16
SP - 2049
EP - 2057
JO - Stem cell reports
JF - Stem cell reports
IS - 9
ER -
