Engineering cartilage tissue by co-culturing of chondrocytes and mesenchymal stromal cells

Yao Fu, Carlo A. Paggi, Amel Dudakovic, Andre J. van Wijnen, Janine N. Post, Marcel Karperien*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

Co-culture of chondrocytes and mesenchymal stromal cells (MSCs) has been shown to be beneficial in engineering cartilage tissue in vitro. In these co-cultures, MSCs increase the proliferation and matrix deposition of chondrocytes. The MSCs accomplish this beneficial effect by so-called trophic actions. Thus, large cartilage constructs can be made with a relatively small number of chondrocytes. In this chapter, we describe different methods for making co-cultures of MSCs and chondrocytes. We also provide detailed protocols for analyzing MSC-chondrocyte co-cultures with cell tracking, proliferation assays, species-specific polymerase chain reactions (PCR), rheological analysis, compression analysis, RNA-sequencing analysis, short tandem repeats analysis, and biochemical examination.

Original languageEnglish
Title of host publicationOsteoporosis and Osteoarthritis
EditorsAndre J. van Wijnen, Marina S. Ganshina
PublisherHumana Press Inc.
Pages53-70
Number of pages18
ISBN (Electronic)978-1-0716-0989-7
ISBN (Print)978-1-0716-0988-0
DOIs
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2221
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cartilage engineering
  • Chondrocytes
  • Co-culture
  • Matrix deposition
  • Mesenchymal stromal cells
  • Trophic effects

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