Engineering cartilage tissue by co-culturing of chondrocytes and mesenchymal stromal cells

Yao Fu, Carlo A. Paggi, Amel Dudakovic, Andre J. van Wijnen, Janine N. Post, Marcel Karperien*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

2 Citations (Scopus)


Co-culture of chondrocytes and mesenchymal stromal cells (MSCs) has been shown to be beneficial in engineering cartilage tissue in vitro. In these co-cultures, MSCs increase the proliferation and matrix deposition of chondrocytes. The MSCs accomplish this beneficial effect by so-called trophic actions. Thus, large cartilage constructs can be made with a relatively small number of chondrocytes. In this chapter, we describe different methods for making co-cultures of MSCs and chondrocytes. We also provide detailed protocols for analyzing MSC-chondrocyte co-cultures with cell tracking, proliferation assays, species-specific polymerase chain reactions (PCR), rheological analysis, compression analysis, RNA-sequencing analysis, short tandem repeats analysis, and biochemical examination.

Original languageEnglish
Title of host publicationOsteoporosis and Osteoarthritis
EditorsAndre J. van Wijnen, Marina S. Ganshina
PublisherHumana Press
Number of pages18
ISBN (Electronic)978-1-0716-0989-7
ISBN (Print)978-1-0716-0988-0
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Cartilage engineering
  • Chondrocytes
  • Co-culture
  • Matrix deposition
  • Mesenchymal stromal cells
  • Trophic effects


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