Engineering Cartilage Tissue by Pellet Coculture of Chondrocytes and Mesenchymal Stromal Cells

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

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Abstract

Coculture of chondrocytes and mesenchymal stromal cells (MSCs) in pellets has been shown to be beneficial in engineering cartilage tissue in vitro. In these cultures trophic effects of MSCs increase the proliferation and matrix deposition of chondrocytes. Thus, large cartilage constructs can be made with a relatively small number of chondrocytes. In this chapter, we describe the methods for making coculture pellets of MSCs and chondrocytes. We also provide detailed protocols for analyzing coculture pellets with cell tracking, proliferation assays, species specific polymerase chain reactions (PCR), short tandem repeats analysis, and histological examination
Original languageEnglish
Title of host publicationOsteoporosis and Osteoarthritis
EditorsJennifer J. Westendorf, Andre J. van Wijnen
Place of PublicationNew York
PublisherSpringer
Pages31-41
Number of pages10
ISBN (Print)978-1-4939-1618-4
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in molecular biology
PublisherSpringer
Number1226
Volume1226
ISSN (Print)1064-3745

Fingerprint

Tissue Engineering
Chondrocytes
Coculture Techniques
Mesenchymal Stromal Cells
Cartilage
Cell Proliferation
Cell Tracking
Microsatellite Repeats
Polymerase Chain Reaction

Keywords

  • IR-95917
  • METIS-310524

Cite this

Wu, L., Post, J. N., & Karperien, H. B. J. (2015). Engineering Cartilage Tissue by Pellet Coculture of Chondrocytes and Mesenchymal Stromal Cells. In J. J. Westendorf, & A. J. van Wijnen (Eds.), Osteoporosis and Osteoarthritis (pp. 31-41). (Methods in molecular biology; Vol. 1226, No. 1226). New York: Springer. https://doi.org/10.1007/978-1-4939-1619-1_4
Wu, Ling ; Post, Janine Nicole ; Karperien, Hermanus Bernardus Johannes. / Engineering Cartilage Tissue by Pellet Coculture of Chondrocytes and Mesenchymal Stromal Cells. Osteoporosis and Osteoarthritis. editor / Jennifer J. Westendorf ; Andre J. van Wijnen. New York : Springer, 2015. pp. 31-41 (Methods in molecular biology; 1226).
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Wu, L, Post, JN & Karperien, HBJ 2015, Engineering Cartilage Tissue by Pellet Coculture of Chondrocytes and Mesenchymal Stromal Cells. in JJ Westendorf & AJ van Wijnen (eds), Osteoporosis and Osteoarthritis. Methods in molecular biology, no. 1226, vol. 1226, Springer, New York, pp. 31-41. https://doi.org/10.1007/978-1-4939-1619-1_4

Engineering Cartilage Tissue by Pellet Coculture of Chondrocytes and Mesenchymal Stromal Cells. / Wu, Ling; Post, Janine Nicole; Karperien, Hermanus Bernardus Johannes.

Osteoporosis and Osteoarthritis. ed. / Jennifer J. Westendorf; Andre J. van Wijnen. New York : Springer, 2015. p. 31-41 (Methods in molecular biology; Vol. 1226, No. 1226).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

TY - CHAP

T1 - Engineering Cartilage Tissue by Pellet Coculture of Chondrocytes and Mesenchymal Stromal Cells

AU - Wu, Ling

AU - Post, Janine Nicole

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N2 - Coculture of chondrocytes and mesenchymal stromal cells (MSCs) in pellets has been shown to be beneficial in engineering cartilage tissue in vitro. In these cultures trophic effects of MSCs increase the proliferation and matrix deposition of chondrocytes. Thus, large cartilage constructs can be made with a relatively small number of chondrocytes. In this chapter, we describe the methods for making coculture pellets of MSCs and chondrocytes. We also provide detailed protocols for analyzing coculture pellets with cell tracking, proliferation assays, species specific polymerase chain reactions (PCR), short tandem repeats analysis, and histological examination

AB - Coculture of chondrocytes and mesenchymal stromal cells (MSCs) in pellets has been shown to be beneficial in engineering cartilage tissue in vitro. In these cultures trophic effects of MSCs increase the proliferation and matrix deposition of chondrocytes. Thus, large cartilage constructs can be made with a relatively small number of chondrocytes. In this chapter, we describe the methods for making coculture pellets of MSCs and chondrocytes. We also provide detailed protocols for analyzing coculture pellets with cell tracking, proliferation assays, species specific polymerase chain reactions (PCR), short tandem repeats analysis, and histological examination

KW - IR-95917

KW - METIS-310524

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DO - 10.1007/978-1-4939-1619-1_4

M3 - Chapter

SN - 978-1-4939-1618-4

T3 - Methods in molecular biology

SP - 31

EP - 41

BT - Osteoporosis and Osteoarthritis

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Wu L, Post JN, Karperien HBJ. Engineering Cartilage Tissue by Pellet Coculture of Chondrocytes and Mesenchymal Stromal Cells. In Westendorf JJ, van Wijnen AJ, editors, Osteoporosis and Osteoarthritis. New York: Springer. 2015. p. 31-41. (Methods in molecular biology; 1226). https://doi.org/10.1007/978-1-4939-1619-1_4