Engineering new bone via a minimally invasive route using human bone marrow derived stromal cell aggregates, micro ceramic particles and human platelet rich plasma gel

Anindita Chatterjea, Huipin Yuan, Supriyo Chatterjea, Henk Garritsen, Auke Renard, Clemens A. van Blitterswijk, Jan de Boer

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    Abstract

    There is a rise in the popularity of arthroscopic procedures in orthopedics. However, the majority of cell based bone tissue engineered constructs rely on solid pre-formed scaffolding materials, which require large incisions and extensive dissections for placement at the defect site. Thus, they are not suitable for minimally invasive techniques. The aim of this study was to develop a clinically relevant, easily moldable, bone tissue engineered construct (TEC), amenable to minimally invasive techniques, using human mesenchymal stromal cells (hMSC) and calcium phosphate micro particles in combination with an in-situ forming platelet rich plasma (PRP) gel obtained from human platelets. Most conventional TECs rely on seeding and culturing single cell suspensions of hMSCs on scaffolds. However, for generating TECs amenable to the minimally invasive approach, it was essential to aggregate the hMSCs in vitro prior to seeding them on the scaffolds as unaggregated MSCs did not generate any bone. 24 hours of in vitro aggregation was determined to be optimal for maintaining cell viability in vitro and bone formation in vivo. Moreover, no statistically significant difference was observed in the amount of bone formed when the TECs were implanted via an open approach or a minimally invasive route. TECs generated using MSCs from three different human donors generated new bone through the minimally invasive route in a reproducible manner, suggesting that these TECs could be a viable alternative to pre-formed scaffolds employed through an open surgery for treating bone defects.
    Original languageEnglish
    Pages (from-to)340-349
    JournalTissue engineering. Part A
    Volume19
    Issue number3-4
    DOIs
    Publication statusPublished - 10 Oct 2013

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    Platelet-Rich Plasma
    Ceramics
    Platelets
    Mesenchymal Stromal Cells
    Particles (particulate matter)
    Bone
    Gels
    Plasmas
    Bone and Bones
    Scaffolds
    Orthopedic Procedures
    Tissue
    Dissection
    Defects
    Osteogenesis
    Orthopedics
    Calcium phosphate
    Cell Survival
    Suspensions
    Surgery

    Keywords

    • METIS-288055
    • IR-81418

    Cite this

    Chatterjea, Anindita ; Yuan, Huipin ; Chatterjea, Supriyo ; Garritsen, Henk ; Renard, Auke ; van Blitterswijk, Clemens A. ; de Boer, Jan. / Engineering new bone via a minimally invasive route using human bone marrow derived stromal cell aggregates, micro ceramic particles and human platelet rich plasma gel. In: Tissue engineering. Part A. 2013 ; Vol. 19, No. 3-4. pp. 340-349.
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    Engineering new bone via a minimally invasive route using human bone marrow derived stromal cell aggregates, micro ceramic particles and human platelet rich plasma gel. / Chatterjea, Anindita; Yuan, Huipin; Chatterjea, Supriyo; Garritsen, Henk; Renard, Auke; van Blitterswijk, Clemens A.; de Boer, Jan.

    In: Tissue engineering. Part A, Vol. 19, No. 3-4, 10.10.2013, p. 340-349.

    Research output: Contribution to journalArticleAcademicpeer-review

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    AU - Chatterjea, Anindita

    AU - Yuan, Huipin

    AU - Chatterjea, Supriyo

    AU - Garritsen, Henk

    AU - Renard, Auke

    AU - van Blitterswijk, Clemens A.

    AU - de Boer, Jan

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    N2 - There is a rise in the popularity of arthroscopic procedures in orthopedics. However, the majority of cell based bone tissue engineered constructs rely on solid pre-formed scaffolding materials, which require large incisions and extensive dissections for placement at the defect site. Thus, they are not suitable for minimally invasive techniques. The aim of this study was to develop a clinically relevant, easily moldable, bone tissue engineered construct (TEC), amenable to minimally invasive techniques, using human mesenchymal stromal cells (hMSC) and calcium phosphate micro particles in combination with an in-situ forming platelet rich plasma (PRP) gel obtained from human platelets. Most conventional TECs rely on seeding and culturing single cell suspensions of hMSCs on scaffolds. However, for generating TECs amenable to the minimally invasive approach, it was essential to aggregate the hMSCs in vitro prior to seeding them on the scaffolds as unaggregated MSCs did not generate any bone. 24 hours of in vitro aggregation was determined to be optimal for maintaining cell viability in vitro and bone formation in vivo. Moreover, no statistically significant difference was observed in the amount of bone formed when the TECs were implanted via an open approach or a minimally invasive route. TECs generated using MSCs from three different human donors generated new bone through the minimally invasive route in a reproducible manner, suggesting that these TECs could be a viable alternative to pre-formed scaffolds employed through an open surgery for treating bone defects.

    AB - There is a rise in the popularity of arthroscopic procedures in orthopedics. However, the majority of cell based bone tissue engineered constructs rely on solid pre-formed scaffolding materials, which require large incisions and extensive dissections for placement at the defect site. Thus, they are not suitable for minimally invasive techniques. The aim of this study was to develop a clinically relevant, easily moldable, bone tissue engineered construct (TEC), amenable to minimally invasive techniques, using human mesenchymal stromal cells (hMSC) and calcium phosphate micro particles in combination with an in-situ forming platelet rich plasma (PRP) gel obtained from human platelets. Most conventional TECs rely on seeding and culturing single cell suspensions of hMSCs on scaffolds. However, for generating TECs amenable to the minimally invasive approach, it was essential to aggregate the hMSCs in vitro prior to seeding them on the scaffolds as unaggregated MSCs did not generate any bone. 24 hours of in vitro aggregation was determined to be optimal for maintaining cell viability in vitro and bone formation in vivo. Moreover, no statistically significant difference was observed in the amount of bone formed when the TECs were implanted via an open approach or a minimally invasive route. TECs generated using MSCs from three different human donors generated new bone through the minimally invasive route in a reproducible manner, suggesting that these TECs could be a viable alternative to pre-formed scaffolds employed through an open surgery for treating bone defects.

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