Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone

Mehdi Kazemzadeh-Narbat, Jeroen Rouwkema, Nasim Annabi, Hao Cheng, Masoumeh Ghaderi, Byung-Hyun Cha, Mansi Aparnathi, Akbar Khalilpour, Batzaya Byambaa, Esmaiel Jabbari, Ali Tamayol, Ali Khademhosseini

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Scopus)
47 Downloads (Pure)

Abstract

Engineering bone tissue requires the generation of a highly organized vasculature. Cellular behavior is affected by the respective niche. Directing cellular behavior and differentiation for creating mineralized regions surrounded by vasculature can be achieved by controlling the pattern of osteogenic and angiogenic niches. This manuscript reports on engineering vascularized bone tissues by incorporating osteogenic and angiogenic cell-laden niches in a photocrosslinkable hydrogel construct. Two-step photolithography process is used to control the stiffness of the hydrogel and distribution of cells in the patterned hydrogel. In addittion, osteoinductive nanoparticles are utilized to induce osteogenesis. The size of microfabricated constructs has a pronounced effect on cellular organization and function. It is shown that the simultaneous presence of both osteogenic and angiogenic niches in one construct results in formation of mineralized regions surrounded by organized vasculature. In addition, the presence of angiogenic niche improves bone formation. This approach can be used for engineered constructs that can be used for treatment of bone defects.
Original languageEnglish
Article number1601122
JournalAdvanced healthcare materials
Volume6
Issue number10
DOIs
Publication statusPublished - 27 Feb 2017

Fingerprint

Hydrogel
Hydrogels
Bone
Osteogenesis
Bone and Bones
Tissue
Nanoparticles
Photolithography
Stiffness
Defects

Cite this

Kazemzadeh-Narbat, M., Rouwkema, J., Annabi, N., Cheng, H., Ghaderi, M., Cha, B-H., ... Khademhosseini, A. (2017). Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone. Advanced healthcare materials, 6(10), [1601122]. https://doi.org/10.1002/adhm.201601122
Kazemzadeh-Narbat, Mehdi ; Rouwkema, Jeroen ; Annabi, Nasim ; Cheng, Hao ; Ghaderi, Masoumeh ; Cha, Byung-Hyun ; Aparnathi, Mansi ; Khalilpour, Akbar ; Byambaa, Batzaya ; Jabbari, Esmaiel ; Tamayol, Ali ; Khademhosseini, Ali. / Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone. In: Advanced healthcare materials. 2017 ; Vol. 6, No. 10.
@article{740561272e21498aacdf4cafcc2ef922,
title = "Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone",
abstract = "Engineering bone tissue requires the generation of a highly organized vasculature. Cellular behavior is affected by the respective niche. Directing cellular behavior and differentiation for creating mineralized regions surrounded by vasculature can be achieved by controlling the pattern of osteogenic and angiogenic niches. This manuscript reports on engineering vascularized bone tissues by incorporating osteogenic and angiogenic cell-laden niches in a photocrosslinkable hydrogel construct. Two-step photolithography process is used to control the stiffness of the hydrogel and distribution of cells in the patterned hydrogel. In addittion, osteoinductive nanoparticles are utilized to induce osteogenesis. The size of microfabricated constructs has a pronounced effect on cellular organization and function. It is shown that the simultaneous presence of both osteogenic and angiogenic niches in one construct results in formation of mineralized regions surrounded by organized vasculature. In addition, the presence of angiogenic niche improves bone formation. This approach can be used for engineered constructs that can be used for treatment of bone defects.",
author = "Mehdi Kazemzadeh-Narbat and Jeroen Rouwkema and Nasim Annabi and Hao Cheng and Masoumeh Ghaderi and Byung-Hyun Cha and Mansi Aparnathi and Akbar Khalilpour and Batzaya Byambaa and Esmaiel Jabbari and Ali Tamayol and Ali Khademhosseini",
year = "2017",
month = "2",
day = "27",
doi = "10.1002/adhm.201601122",
language = "English",
volume = "6",
journal = "Advanced healthcare materials",
issn = "2192-2640",
publisher = "Wiley",
number = "10",

}

Kazemzadeh-Narbat, M, Rouwkema, J, Annabi, N, Cheng, H, Ghaderi, M, Cha, B-H, Aparnathi, M, Khalilpour, A, Byambaa, B, Jabbari, E, Tamayol, A & Khademhosseini, A 2017, 'Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone' Advanced healthcare materials, vol. 6, no. 10, 1601122. https://doi.org/10.1002/adhm.201601122

Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone. / Kazemzadeh-Narbat, Mehdi; Rouwkema, Jeroen ; Annabi, Nasim; Cheng, Hao; Ghaderi, Masoumeh; Cha, Byung-Hyun; Aparnathi, Mansi; Khalilpour, Akbar; Byambaa, Batzaya; Jabbari, Esmaiel; Tamayol, Ali; Khademhosseini, Ali.

In: Advanced healthcare materials, Vol. 6, No. 10, 1601122, 27.02.2017.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone

AU - Kazemzadeh-Narbat, Mehdi

AU - Rouwkema, Jeroen

AU - Annabi, Nasim

AU - Cheng, Hao

AU - Ghaderi, Masoumeh

AU - Cha, Byung-Hyun

AU - Aparnathi, Mansi

AU - Khalilpour, Akbar

AU - Byambaa, Batzaya

AU - Jabbari, Esmaiel

AU - Tamayol, Ali

AU - Khademhosseini, Ali

PY - 2017/2/27

Y1 - 2017/2/27

N2 - Engineering bone tissue requires the generation of a highly organized vasculature. Cellular behavior is affected by the respective niche. Directing cellular behavior and differentiation for creating mineralized regions surrounded by vasculature can be achieved by controlling the pattern of osteogenic and angiogenic niches. This manuscript reports on engineering vascularized bone tissues by incorporating osteogenic and angiogenic cell-laden niches in a photocrosslinkable hydrogel construct. Two-step photolithography process is used to control the stiffness of the hydrogel and distribution of cells in the patterned hydrogel. In addittion, osteoinductive nanoparticles are utilized to induce osteogenesis. The size of microfabricated constructs has a pronounced effect on cellular organization and function. It is shown that the simultaneous presence of both osteogenic and angiogenic niches in one construct results in formation of mineralized regions surrounded by organized vasculature. In addition, the presence of angiogenic niche improves bone formation. This approach can be used for engineered constructs that can be used for treatment of bone defects.

AB - Engineering bone tissue requires the generation of a highly organized vasculature. Cellular behavior is affected by the respective niche. Directing cellular behavior and differentiation for creating mineralized regions surrounded by vasculature can be achieved by controlling the pattern of osteogenic and angiogenic niches. This manuscript reports on engineering vascularized bone tissues by incorporating osteogenic and angiogenic cell-laden niches in a photocrosslinkable hydrogel construct. Two-step photolithography process is used to control the stiffness of the hydrogel and distribution of cells in the patterned hydrogel. In addittion, osteoinductive nanoparticles are utilized to induce osteogenesis. The size of microfabricated constructs has a pronounced effect on cellular organization and function. It is shown that the simultaneous presence of both osteogenic and angiogenic niches in one construct results in formation of mineralized regions surrounded by organized vasculature. In addition, the presence of angiogenic niche improves bone formation. This approach can be used for engineered constructs that can be used for treatment of bone defects.

U2 - 10.1002/adhm.201601122

DO - 10.1002/adhm.201601122

M3 - Article

VL - 6

JO - Advanced healthcare materials

JF - Advanced healthcare materials

SN - 2192-2640

IS - 10

M1 - 1601122

ER -