Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone

Mehdi Kazemzadeh-Narbat, Jeroen Rouwkema, Nasim Annabi, Hao Cheng, Masoumeh Ghaderi, Byung-Hyun Cha, Mansi Aparnathi, Akbar Khalilpour, Batzaya Byambaa, Esmaiel Jabbari, Ali Tamayol, Ali Khademhosseini

    Research output: Contribution to journalArticleAcademicpeer-review

    62 Citations (Scopus)
    180 Downloads (Pure)


    Engineering bone tissue requires the generation of a highly organized vasculature. Cellular behavior is affected by the respective niche. Directing cellular behavior and differentiation for creating mineralized regions surrounded by vasculature can be achieved by controlling the pattern of osteogenic and angiogenic niches. This manuscript reports on engineering vascularized bone tissues by incorporating osteogenic and angiogenic cell-laden niches in a photocrosslinkable hydrogel construct. Two-step photolithography process is used to control the stiffness of the hydrogel and distribution of cells in the patterned hydrogel. In addittion, osteoinductive nanoparticles are utilized to induce osteogenesis. The size of microfabricated constructs has a pronounced effect on cellular organization and function. It is shown that the simultaneous presence of both osteogenic and angiogenic niches in one construct results in formation of mineralized regions surrounded by organized vasculature. In addition, the presence of angiogenic niche improves bone formation. This approach can be used for engineered constructs that can be used for treatment of bone defects.
    Original languageEnglish
    Article number1601122
    JournalAdvanced healthcare materials
    Issue number10
    Publication statusPublished - 27 Feb 2017


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