Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone

Mehdi Kazemzadeh-Narbat, Jeroen Rouwkema, Nasim Annabi, Hao Cheng, Masoumeh Ghaderi, Byung-Hyun Cha, Mansi Aparnathi, Akbar Khalilpour, Batzaya Byambaa, Esmaiel Jabbari, Ali Tamayol, Ali Khademhosseini

    Research output: Contribution to journalArticleAcademicpeer-review

    26 Citations (Scopus)
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    Abstract

    Engineering bone tissue requires the generation of a highly organized vasculature. Cellular behavior is affected by the respective niche. Directing cellular behavior and differentiation for creating mineralized regions surrounded by vasculature can be achieved by controlling the pattern of osteogenic and angiogenic niches. This manuscript reports on engineering vascularized bone tissues by incorporating osteogenic and angiogenic cell-laden niches in a photocrosslinkable hydrogel construct. Two-step photolithography process is used to control the stiffness of the hydrogel and distribution of cells in the patterned hydrogel. In addittion, osteoinductive nanoparticles are utilized to induce osteogenesis. The size of microfabricated constructs has a pronounced effect on cellular organization and function. It is shown that the simultaneous presence of both osteogenic and angiogenic niches in one construct results in formation of mineralized regions surrounded by organized vasculature. In addition, the presence of angiogenic niche improves bone formation. This approach can be used for engineered constructs that can be used for treatment of bone defects.
    Original languageEnglish
    Article number1601122
    JournalAdvanced healthcare materials
    Volume6
    Issue number10
    DOIs
    Publication statusPublished - 27 Feb 2017

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    Hydrogel
    Hydrogels
    Bone
    Osteogenesis
    Bone and Bones
    Tissue
    Nanoparticles
    Photolithography
    Stiffness
    Defects

    Cite this

    Kazemzadeh-Narbat, M., Rouwkema, J., Annabi, N., Cheng, H., Ghaderi, M., Cha, B-H., ... Khademhosseini, A. (2017). Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone. Advanced healthcare materials, 6(10), [1601122]. https://doi.org/10.1002/adhm.201601122
    Kazemzadeh-Narbat, Mehdi ; Rouwkema, Jeroen ; Annabi, Nasim ; Cheng, Hao ; Ghaderi, Masoumeh ; Cha, Byung-Hyun ; Aparnathi, Mansi ; Khalilpour, Akbar ; Byambaa, Batzaya ; Jabbari, Esmaiel ; Tamayol, Ali ; Khademhosseini, Ali. / Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone. In: Advanced healthcare materials. 2017 ; Vol. 6, No. 10.
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    abstract = "Engineering bone tissue requires the generation of a highly organized vasculature. Cellular behavior is affected by the respective niche. Directing cellular behavior and differentiation for creating mineralized regions surrounded by vasculature can be achieved by controlling the pattern of osteogenic and angiogenic niches. This manuscript reports on engineering vascularized bone tissues by incorporating osteogenic and angiogenic cell-laden niches in a photocrosslinkable hydrogel construct. Two-step photolithography process is used to control the stiffness of the hydrogel and distribution of cells in the patterned hydrogel. In addittion, osteoinductive nanoparticles are utilized to induce osteogenesis. The size of microfabricated constructs has a pronounced effect on cellular organization and function. It is shown that the simultaneous presence of both osteogenic and angiogenic niches in one construct results in formation of mineralized regions surrounded by organized vasculature. In addition, the presence of angiogenic niche improves bone formation. This approach can be used for engineered constructs that can be used for treatment of bone defects.",
    author = "Mehdi Kazemzadeh-Narbat and Jeroen Rouwkema and Nasim Annabi and Hao Cheng and Masoumeh Ghaderi and Byung-Hyun Cha and Mansi Aparnathi and Akbar Khalilpour and Batzaya Byambaa and Esmaiel Jabbari and Ali Tamayol and Ali Khademhosseini",
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    Kazemzadeh-Narbat, M, Rouwkema, J, Annabi, N, Cheng, H, Ghaderi, M, Cha, B-H, Aparnathi, M, Khalilpour, A, Byambaa, B, Jabbari, E, Tamayol, A & Khademhosseini, A 2017, 'Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone', Advanced healthcare materials, vol. 6, no. 10, 1601122. https://doi.org/10.1002/adhm.201601122

    Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone. / Kazemzadeh-Narbat, Mehdi; Rouwkema, Jeroen ; Annabi, Nasim; Cheng, Hao; Ghaderi, Masoumeh; Cha, Byung-Hyun; Aparnathi, Mansi; Khalilpour, Akbar; Byambaa, Batzaya; Jabbari, Esmaiel; Tamayol, Ali; Khademhosseini, Ali.

    In: Advanced healthcare materials, Vol. 6, No. 10, 1601122, 27.02.2017.

    Research output: Contribution to journalArticleAcademicpeer-review

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    T1 - Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone

    AU - Kazemzadeh-Narbat, Mehdi

    AU - Rouwkema, Jeroen

    AU - Annabi, Nasim

    AU - Cheng, Hao

    AU - Ghaderi, Masoumeh

    AU - Cha, Byung-Hyun

    AU - Aparnathi, Mansi

    AU - Khalilpour, Akbar

    AU - Byambaa, Batzaya

    AU - Jabbari, Esmaiel

    AU - Tamayol, Ali

    AU - Khademhosseini, Ali

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    AB - Engineering bone tissue requires the generation of a highly organized vasculature. Cellular behavior is affected by the respective niche. Directing cellular behavior and differentiation for creating mineralized regions surrounded by vasculature can be achieved by controlling the pattern of osteogenic and angiogenic niches. This manuscript reports on engineering vascularized bone tissues by incorporating osteogenic and angiogenic cell-laden niches in a photocrosslinkable hydrogel construct. Two-step photolithography process is used to control the stiffness of the hydrogel and distribution of cells in the patterned hydrogel. In addittion, osteoinductive nanoparticles are utilized to induce osteogenesis. The size of microfabricated constructs has a pronounced effect on cellular organization and function. It is shown that the simultaneous presence of both osteogenic and angiogenic niches in one construct results in formation of mineralized regions surrounded by organized vasculature. In addition, the presence of angiogenic niche improves bone formation. This approach can be used for engineered constructs that can be used for treatment of bone defects.

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