Enhanced in vitro and in vivo cellular imaging with green tea coated water-soluble iron oxide nanocrystals

Lisong Xiao, Marianne Mertens, Laura Wortmann, Silke Kremer, Martin Valldor, Twan Gerardus Gertudis Maria Lammers, Fabian Kiessling, Sanjay Mathur

Research output: Contribution to journalArticleAcademicpeer-review

29 Citations (Scopus)

Abstract

Fully green and facile redox chemistry involving reduction of colloidal iron hydroxide (Fe(OH)3) through green tea (GT) polyphenols produced water-soluble Fe3O4 nanocrystals coated with GT extracts namely epigallocatechin gallate (EGCG) and epicatechin (EC). Electron donating polyphenols stoichiometrically reduced Fe3+ ions into Fe2+ ions resulting in the formation of magnetite (Fe3O4) nanoparticles and corresponding oxidized products (semiquinones and quinones) that simultaneously served as efficient surface chelators for the Fe3O4 nanoparticles making them dispersible and stable in water, PBS, and cell culture medium for extended time periods. As-formed iron oxide nanoparticles (2.5–6 nm) displayed high crystallinity and saturation magnetization as well as high relaxivity ratios manifested in strong contrast enhancement observed in T2-weighted images. Potential of green tea-coated superparamagnetic iron oxide nanocrystals (SPIONs) as superior negative contrast agents was confirmed by in vitro and in vivo experiments. Primary human macrophages (J774A.1) and colon cancer cells (CT26) were chosen to assess cytotoxicity and cellular uptake of GT-, EGCGq-, and ECq-coated Fe3O4 nanoparticles, which showed high uptake efficiencies by J774A.1 and CT26 cells without any additional transfection agent. Furthermore, the in vivo accumulation characteristics of GT-coated Fe3O4 nanoparticles were similar to those observed in clinical studies of SPIONs with comparable accumulation in epidermoid cancer-xenograft bearing mice. Given their promising transport and uptake characteristics and new surface chemistry, GT-SPIONs conjugates can be applied for multimodal imaging and therapeutic applications by anchoring further functionalities.
Original languageUndefined
Pages (from-to)6530-6540
JournalACS applied materials & interfaces
Volume7
Issue number12
DOIs
Publication statusPublished - 2015

Keywords

  • METIS-315280
  • IR-99953

Cite this

Xiao, Lisong ; Mertens, Marianne ; Wortmann, Laura ; Kremer, Silke ; Valldor, Martin ; Lammers, Twan Gerardus Gertudis Maria ; Kiessling, Fabian ; Mathur, Sanjay. / Enhanced in vitro and in vivo cellular imaging with green tea coated water-soluble iron oxide nanocrystals. In: ACS applied materials & interfaces. 2015 ; Vol. 7, No. 12. pp. 6530-6540.
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title = "Enhanced in vitro and in vivo cellular imaging with green tea coated water-soluble iron oxide nanocrystals",
abstract = "Fully green and facile redox chemistry involving reduction of colloidal iron hydroxide (Fe(OH)3) through green tea (GT) polyphenols produced water-soluble Fe3O4 nanocrystals coated with GT extracts namely epigallocatechin gallate (EGCG) and epicatechin (EC). Electron donating polyphenols stoichiometrically reduced Fe3+ ions into Fe2+ ions resulting in the formation of magnetite (Fe3O4) nanoparticles and corresponding oxidized products (semiquinones and quinones) that simultaneously served as efficient surface chelators for the Fe3O4 nanoparticles making them dispersible and stable in water, PBS, and cell culture medium for extended time periods. As-formed iron oxide nanoparticles (2.5–6 nm) displayed high crystallinity and saturation magnetization as well as high relaxivity ratios manifested in strong contrast enhancement observed in T2-weighted images. Potential of green tea-coated superparamagnetic iron oxide nanocrystals (SPIONs) as superior negative contrast agents was confirmed by in vitro and in vivo experiments. Primary human macrophages (J774A.1) and colon cancer cells (CT26) were chosen to assess cytotoxicity and cellular uptake of GT-, EGCGq-, and ECq-coated Fe3O4 nanoparticles, which showed high uptake efficiencies by J774A.1 and CT26 cells without any additional transfection agent. Furthermore, the in vivo accumulation characteristics of GT-coated Fe3O4 nanoparticles were similar to those observed in clinical studies of SPIONs with comparable accumulation in epidermoid cancer-xenograft bearing mice. Given their promising transport and uptake characteristics and new surface chemistry, GT-SPIONs conjugates can be applied for multimodal imaging and therapeutic applications by anchoring further functionalities.",
keywords = "METIS-315280, IR-99953",
author = "Lisong Xiao and Marianne Mertens and Laura Wortmann and Silke Kremer and Martin Valldor and Lammers, {Twan Gerardus Gertudis Maria} and Fabian Kiessling and Sanjay Mathur",
year = "2015",
doi = "10.1021/am508404t",
language = "Undefined",
volume = "7",
pages = "6530--6540",
journal = "ACS applied materials & interfaces",
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Enhanced in vitro and in vivo cellular imaging with green tea coated water-soluble iron oxide nanocrystals. / Xiao, Lisong; Mertens, Marianne; Wortmann, Laura; Kremer, Silke; Valldor, Martin; Lammers, Twan Gerardus Gertudis Maria; Kiessling, Fabian; Mathur, Sanjay.

In: ACS applied materials & interfaces, Vol. 7, No. 12, 2015, p. 6530-6540.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Enhanced in vitro and in vivo cellular imaging with green tea coated water-soluble iron oxide nanocrystals

AU - Xiao, Lisong

AU - Mertens, Marianne

AU - Wortmann, Laura

AU - Kremer, Silke

AU - Valldor, Martin

AU - Lammers, Twan Gerardus Gertudis Maria

AU - Kiessling, Fabian

AU - Mathur, Sanjay

PY - 2015

Y1 - 2015

N2 - Fully green and facile redox chemistry involving reduction of colloidal iron hydroxide (Fe(OH)3) through green tea (GT) polyphenols produced water-soluble Fe3O4 nanocrystals coated with GT extracts namely epigallocatechin gallate (EGCG) and epicatechin (EC). Electron donating polyphenols stoichiometrically reduced Fe3+ ions into Fe2+ ions resulting in the formation of magnetite (Fe3O4) nanoparticles and corresponding oxidized products (semiquinones and quinones) that simultaneously served as efficient surface chelators for the Fe3O4 nanoparticles making them dispersible and stable in water, PBS, and cell culture medium for extended time periods. As-formed iron oxide nanoparticles (2.5–6 nm) displayed high crystallinity and saturation magnetization as well as high relaxivity ratios manifested in strong contrast enhancement observed in T2-weighted images. Potential of green tea-coated superparamagnetic iron oxide nanocrystals (SPIONs) as superior negative contrast agents was confirmed by in vitro and in vivo experiments. Primary human macrophages (J774A.1) and colon cancer cells (CT26) were chosen to assess cytotoxicity and cellular uptake of GT-, EGCGq-, and ECq-coated Fe3O4 nanoparticles, which showed high uptake efficiencies by J774A.1 and CT26 cells without any additional transfection agent. Furthermore, the in vivo accumulation characteristics of GT-coated Fe3O4 nanoparticles were similar to those observed in clinical studies of SPIONs with comparable accumulation in epidermoid cancer-xenograft bearing mice. Given their promising transport and uptake characteristics and new surface chemistry, GT-SPIONs conjugates can be applied for multimodal imaging and therapeutic applications by anchoring further functionalities.

AB - Fully green and facile redox chemistry involving reduction of colloidal iron hydroxide (Fe(OH)3) through green tea (GT) polyphenols produced water-soluble Fe3O4 nanocrystals coated with GT extracts namely epigallocatechin gallate (EGCG) and epicatechin (EC). Electron donating polyphenols stoichiometrically reduced Fe3+ ions into Fe2+ ions resulting in the formation of magnetite (Fe3O4) nanoparticles and corresponding oxidized products (semiquinones and quinones) that simultaneously served as efficient surface chelators for the Fe3O4 nanoparticles making them dispersible and stable in water, PBS, and cell culture medium for extended time periods. As-formed iron oxide nanoparticles (2.5–6 nm) displayed high crystallinity and saturation magnetization as well as high relaxivity ratios manifested in strong contrast enhancement observed in T2-weighted images. Potential of green tea-coated superparamagnetic iron oxide nanocrystals (SPIONs) as superior negative contrast agents was confirmed by in vitro and in vivo experiments. Primary human macrophages (J774A.1) and colon cancer cells (CT26) were chosen to assess cytotoxicity and cellular uptake of GT-, EGCGq-, and ECq-coated Fe3O4 nanoparticles, which showed high uptake efficiencies by J774A.1 and CT26 cells without any additional transfection agent. Furthermore, the in vivo accumulation characteristics of GT-coated Fe3O4 nanoparticles were similar to those observed in clinical studies of SPIONs with comparable accumulation in epidermoid cancer-xenograft bearing mice. Given their promising transport and uptake characteristics and new surface chemistry, GT-SPIONs conjugates can be applied for multimodal imaging and therapeutic applications by anchoring further functionalities.

KW - METIS-315280

KW - IR-99953

U2 - 10.1021/am508404t

DO - 10.1021/am508404t

M3 - Article

VL - 7

SP - 6530

EP - 6540

JO - ACS applied materials & interfaces

JF - ACS applied materials & interfaces

SN - 1944-8244

IS - 12

ER -