Enhanced mechanical and cell adhesive properties of photo-crosslinked PEG hydrogels by incorporation of gelatin in the networks

Jia Liang, Zhengchao Guo, Aimée Timmerman, Dirk Grijpma, André Poot (Corresponding Author)

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)
2 Downloads (Pure)

Abstract

Although synthetic polymers may have suitable physicochemical properties for biomedical applications, biological properties are generally lacking. Poly(ethylene glycol) (PEG) is a frequently used polymer for the preparation of hydrogels. Due to its hydrophilic character, however, cellular interactions with PEG hydrogels are minimal or absent. To improve the cell adhesive properties of PEG hydrogels, we developed hybrid hydrogels based on PEG and the natural polymer gelatin. PEG dimethacrylate (PEG-dMA) and gelatin methacrylate (GelMA) macromers were prepared, which were photo-crosslinked in water in different ratios (75:25, 50:50 and 25:75% (v/v)). The obtained hybrid networks showed macrophase separation, which could be prevented by photo-crosslinking in 0.5% (v/v) acetic acid in water. The toughness of 50:50% PEG-dMA:GelMA hydrogels prepared in 0.5% acetic acid was 2.5 times higher than that of single polymer hydrogels made of PEG-dMA or GelMA. Hybrid hydrogels crosslinked in 0.5% acetic acid supported the proliferation of human mesenchymal stem cells to the same extent as compared to 100% gelatin hydrogel, whereas the cells did not proliferate on 100% PEG hydrogel. In conclusion, our results show that both the cell adhesive and mechanical properties of a photo-crosslinked PEG network can be improved by incorporation of gelatin in the network.

Original languageEnglish
Article number024102
Number of pages9
JournalBiomedical materials (Bristol, England)
Volume14
Issue number2
DOIs
Publication statusPublished - 4 Jan 2019

Fingerprint

Hydrogels
Gelatin
Polyethylene glycols
Adhesives
Methacrylates
Acetic acid
Acetic Acid
Polymers
Hydrogel
Natural polymers
Water
Stem cells
Crosslinking
Toughness
Mechanical properties

Keywords

  • methacrylated PEG
  • methacrylated gelatin
  • photo-crosslinking
  • hydrogel

Cite this

@article{a141a98944cc41b8ba4d2664466d7080,
title = "Enhanced mechanical and cell adhesive properties of photo-crosslinked PEG hydrogels by incorporation of gelatin in the networks",
abstract = "Although synthetic polymers may have suitable physicochemical properties for biomedical applications, biological properties are generally lacking. Poly(ethylene glycol) (PEG) is a frequently used polymer for the preparation of hydrogels. Due to its hydrophilic character, however, cellular interactions with PEG hydrogels are minimal or absent. To improve the cell adhesive properties of PEG hydrogels, we developed hybrid hydrogels based on PEG and the natural polymer gelatin. PEG dimethacrylate (PEG-dMA) and gelatin methacrylate (GelMA) macromers were prepared, which were photo-crosslinked in water in different ratios (75:25, 50:50 and 25:75{\%} (v/v)). The obtained hybrid networks showed macrophase separation, which could be prevented by photo-crosslinking in 0.5{\%} (v/v) acetic acid in water. The toughness of 50:50{\%} PEG-dMA:GelMA hydrogels prepared in 0.5{\%} acetic acid was 2.5 times higher than that of single polymer hydrogels made of PEG-dMA or GelMA. Hybrid hydrogels crosslinked in 0.5{\%} acetic acid supported the proliferation of human mesenchymal stem cells to the same extent as compared to 100{\%} gelatin hydrogel, whereas the cells did not proliferate on 100{\%} PEG hydrogel. In conclusion, our results show that both the cell adhesive and mechanical properties of a photo-crosslinked PEG network can be improved by incorporation of gelatin in the network.",
keywords = "methacrylated PEG, methacrylated gelatin, photo-crosslinking, hydrogel",
author = "Jia Liang and Zhengchao Guo and Aim{\'e}e Timmerman and Dirk Grijpma and Andr{\'e} Poot",
year = "2019",
month = "1",
day = "4",
doi = "10.1088/1748-605X/aaf31b",
language = "English",
volume = "14",
journal = "Biomedical materials",
issn = "1748-6041",
publisher = "IOP Publishing Ltd.",
number = "2",

}

TY - JOUR

T1 - Enhanced mechanical and cell adhesive properties of photo-crosslinked PEG hydrogels by incorporation of gelatin in the networks

AU - Liang, Jia

AU - Guo, Zhengchao

AU - Timmerman, Aimée

AU - Grijpma, Dirk

AU - Poot, André

PY - 2019/1/4

Y1 - 2019/1/4

N2 - Although synthetic polymers may have suitable physicochemical properties for biomedical applications, biological properties are generally lacking. Poly(ethylene glycol) (PEG) is a frequently used polymer for the preparation of hydrogels. Due to its hydrophilic character, however, cellular interactions with PEG hydrogels are minimal or absent. To improve the cell adhesive properties of PEG hydrogels, we developed hybrid hydrogels based on PEG and the natural polymer gelatin. PEG dimethacrylate (PEG-dMA) and gelatin methacrylate (GelMA) macromers were prepared, which were photo-crosslinked in water in different ratios (75:25, 50:50 and 25:75% (v/v)). The obtained hybrid networks showed macrophase separation, which could be prevented by photo-crosslinking in 0.5% (v/v) acetic acid in water. The toughness of 50:50% PEG-dMA:GelMA hydrogels prepared in 0.5% acetic acid was 2.5 times higher than that of single polymer hydrogels made of PEG-dMA or GelMA. Hybrid hydrogels crosslinked in 0.5% acetic acid supported the proliferation of human mesenchymal stem cells to the same extent as compared to 100% gelatin hydrogel, whereas the cells did not proliferate on 100% PEG hydrogel. In conclusion, our results show that both the cell adhesive and mechanical properties of a photo-crosslinked PEG network can be improved by incorporation of gelatin in the network.

AB - Although synthetic polymers may have suitable physicochemical properties for biomedical applications, biological properties are generally lacking. Poly(ethylene glycol) (PEG) is a frequently used polymer for the preparation of hydrogels. Due to its hydrophilic character, however, cellular interactions with PEG hydrogels are minimal or absent. To improve the cell adhesive properties of PEG hydrogels, we developed hybrid hydrogels based on PEG and the natural polymer gelatin. PEG dimethacrylate (PEG-dMA) and gelatin methacrylate (GelMA) macromers were prepared, which were photo-crosslinked in water in different ratios (75:25, 50:50 and 25:75% (v/v)). The obtained hybrid networks showed macrophase separation, which could be prevented by photo-crosslinking in 0.5% (v/v) acetic acid in water. The toughness of 50:50% PEG-dMA:GelMA hydrogels prepared in 0.5% acetic acid was 2.5 times higher than that of single polymer hydrogels made of PEG-dMA or GelMA. Hybrid hydrogels crosslinked in 0.5% acetic acid supported the proliferation of human mesenchymal stem cells to the same extent as compared to 100% gelatin hydrogel, whereas the cells did not proliferate on 100% PEG hydrogel. In conclusion, our results show that both the cell adhesive and mechanical properties of a photo-crosslinked PEG network can be improved by incorporation of gelatin in the network.

KW - methacrylated PEG

KW - methacrylated gelatin

KW - photo-crosslinking

KW - hydrogel

UR - http://www.scopus.com/inward/record.url?scp=85059500084&partnerID=8YFLogxK

U2 - 10.1088/1748-605X/aaf31b

DO - 10.1088/1748-605X/aaf31b

M3 - Article

VL - 14

JO - Biomedical materials

JF - Biomedical materials

SN - 1748-6041

IS - 2

M1 - 024102

ER -