Enhancing Cellular Internalization of Single-Chain Polymer Nanoparticles via Polyplex Formation

Naomi M. Hamelmann; Sjoerd Uijttewaal; Sry D. Hujaya; Jos M.J. Paulusse

doi:10.1021/acs.biomac.2c00858

Enhancing Cellular Internalization of Single-Chain Polymer Nanoparticles via Polyplex Formation

Naomi M. Hamelmann, Sjoerd Uijttewaal, Sry D. Hujaya, Jos M.J. Paulusse^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

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Abstract

Intracellular delivery of nanoparticles is crucial in nanomedicine to reach optimal delivery of therapeutics and imaging agents. Single-chain polymer nanoparticles (SCNPs) are an interesting class of nanoparticles due to their unique site range of 5-20 nm. The intracellular delivery of SCNPs can be enhanced by using delivery agents. Here, a positive polymer is used to form polyplexes with SCNPs, similar to the strategy of protein and gene delivery. The size and surface charge of the polyplexes were evaluated. The cellular uptake showed rapid uptake of SCNPs via polyplex formation, and the cytosolic delivery of the SCNPs was presented by confocal microscopy. The ability of SCNPs to act as nanocarriers was further explored by conjugation of doxorubicin.

Original language	English
Pages (from-to)	5036-5042
Number of pages	7
Journal	Biomacromolecules
Volume	23
Issue number	12
DOIs	https://doi.org/10.1021/acs.biomac.2c00858
Publication status	Published - 12 Dec 2022

Keywords

UT-Hybrid-D

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abstract = "Intracellular delivery of nanoparticles is crucial in nanomedicine to reach optimal delivery of therapeutics and imaging agents. Single-chain polymer nanoparticles (SCNPs) are an interesting class of nanoparticles due to their unique site range of 5-20 nm. The intracellular delivery of SCNPs can be enhanced by using delivery agents. Here, a positive polymer is used to form polyplexes with SCNPs, similar to the strategy of protein and gene delivery. The size and surface charge of the polyplexes were evaluated. The cellular uptake showed rapid uptake of SCNPs via polyplex formation, and the cytosolic delivery of the SCNPs was presented by confocal microscopy. The ability of SCNPs to act as nanocarriers was further explored by conjugation of doxorubicin.",

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AU - Hujaya, Sry D.

AU - Paulusse, Jos M.J.

N1 - Funding Information: This research was funded through the EuroNanoMed III research program (Ref. EURO-NANOMED2017-178) and by Alzheimer Netherlands and co-funded by the PPP Allowance made available by Health∼Holland, Top Sector Life Sciences & Health to stimulate public–private partnerships. Publisher Copyright: © 2022 American Chemical Society.

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Enhancing Cellular Internalization of Single-Chain Polymer Nanoparticles via Polyplex Formation

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