TY - JOUR
T1 - Enhancing volumetric muscle loss (VML) recovery in a rat model using super durable hydrogels derived from bacteria
AU - Niknezhad, Seyyed Vahid
AU - Mehrali, Mehdi
AU - Khorasgani, Farinaz Riyahi
AU - Heidari, Reza
AU - Kadumudi, Firoz Babu
AU - Golafshan, Nasim
AU - Castilho, Miguel
AU - Pennisi, Cristian Pablo
AU - Hasany, Masoud
AU - Jahanshahi, Mohammadjavad
AU - Mehrali, Mohammad
AU - Ghasemi, Younes
AU - Azarpira, Negar
AU - Andresen, Thomas L.
AU - Dolatshahi-Pirouz, Alireza
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/8
Y1 - 2024/8
N2 - Bacteria can be programmed to deliver natural materials with defined biological and mechanical properties for controlling cell growth and differentiation. Here, we present an elastic, resilient and bioactive polysaccharide derived from the extracellular matrix of Pantoea sp. BCCS 001. Specifically, it was methacrylated to generate a new photo crosslinkable hydrogel that we coined Pantoan Methacrylate or put simply PAMA. We have used it for the first time as a tissue engineering hydrogel to treat VML injuries in rats. The crosslinked PAMA hydrogel was super elastic with a recovery nearing 100 %, while mimicking the mechanical stiffness of native muscle. After inclusion of thiolated gelatin via a Michaelis reaction with acrylate groups on PAMA we could also guide muscle progenitor cells into fused and aligned tubes – something reminiscent of mature muscle cells. These results were complemented by sarcomeric alpha-actinin immunostaining studies. Importantly, the implanted hydrogels exhibited almost 2-fold more muscle formation and 50 % less fibrous tissue formation compared to untreated rat groups. In vivo inflammation and toxicity assays likewise gave rise to positive results confirming the biocompatibility of this new biomaterial system. Overall, our results demonstrate that programmable polysaccharides derived from bacteria can be used to further advance the field of tissue engineering. In greater detail, they could in the foreseeable future be used in practical therapies against VML.
AB - Bacteria can be programmed to deliver natural materials with defined biological and mechanical properties for controlling cell growth and differentiation. Here, we present an elastic, resilient and bioactive polysaccharide derived from the extracellular matrix of Pantoea sp. BCCS 001. Specifically, it was methacrylated to generate a new photo crosslinkable hydrogel that we coined Pantoan Methacrylate or put simply PAMA. We have used it for the first time as a tissue engineering hydrogel to treat VML injuries in rats. The crosslinked PAMA hydrogel was super elastic with a recovery nearing 100 %, while mimicking the mechanical stiffness of native muscle. After inclusion of thiolated gelatin via a Michaelis reaction with acrylate groups on PAMA we could also guide muscle progenitor cells into fused and aligned tubes – something reminiscent of mature muscle cells. These results were complemented by sarcomeric alpha-actinin immunostaining studies. Importantly, the implanted hydrogels exhibited almost 2-fold more muscle formation and 50 % less fibrous tissue formation compared to untreated rat groups. In vivo inflammation and toxicity assays likewise gave rise to positive results confirming the biocompatibility of this new biomaterial system. Overall, our results demonstrate that programmable polysaccharides derived from bacteria can be used to further advance the field of tissue engineering. In greater detail, they could in the foreseeable future be used in practical therapies against VML.
UR - http://www.scopus.com/inward/record.url?scp=85194588461&partnerID=8YFLogxK
U2 - 10.1016/j.bioactmat.2024.04.006
DO - 10.1016/j.bioactmat.2024.04.006
M3 - Article
AN - SCOPUS:85194588461
SN - 2452-199X
VL - 38
SP - 540
EP - 558
JO - Bioactive Materials
JF - Bioactive Materials
ER -