Enzymatic control of chitosan gelation for delivery of periodontal ligament cells

Xiang Zhen Yan, Arnold W.G. Nijhuis, Jeroen J.J.P. Van Den Beucken, Sanne K. Both, John A. Jansen, Sander C.G. Leeuwenburgh, Fang Yang

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

The aim of this study is to optimize enzymatic control over gelation of chitosan-based hydrogels for the delivery of periodontal ligament cells (PDLCs). The results reveal that the gelation time, strength, and degradation rate of the chitosan hydrogels can be controlled precisely by variation of the urea and urease concentrations. PDLCs remain viable inside these hydrogels for up to 30 days. Cells released from the hydrogel upon degradation and collected after 3, 15, and 30 days are able to form colonies and osteogenically differentiate. In conclusion, the enzymatic control over the gelation of chitosan hydrogels offers options for the delivery of PDLCs. Physically crosslinked chitosan hydrogels for biomedical purpose can be easily fabricated via enzymatic control. The gelation time, strength, and degradation rate of chitosan hydrogels can be fine-tuned through variation of the concentrations of urea and urease. Periodontal ligament cells remain viable and maintain their capacities upon encapsulation within these hydrogels for up to 30 days.

Original languageEnglish
Pages (from-to)1004-1014
Number of pages11
JournalMacromolecular bioscience
Volume14
Issue number7
DOIs
Publication statusPublished - 1 Jan 2014
Externally publishedYes

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Periodontal Ligament
Hydrogels
Ligaments
Chitosan
Gelation
Urease
Degradation
Urea
Hydrogel
Encapsulation

Keywords

  • cell encapsulation
  • chitosan
  • hydrogels
  • periodontium

Cite this

Yan, X. Z., Nijhuis, A. W. G., Van Den Beucken, J. J. J. P., Both, S. K., Jansen, J. A., Leeuwenburgh, S. C. G., & Yang, F. (2014). Enzymatic control of chitosan gelation for delivery of periodontal ligament cells. Macromolecular bioscience, 14(7), 1004-1014. https://doi.org/10.1002/mabi.201400040
Yan, Xiang Zhen ; Nijhuis, Arnold W.G. ; Van Den Beucken, Jeroen J.J.P. ; Both, Sanne K. ; Jansen, John A. ; Leeuwenburgh, Sander C.G. ; Yang, Fang. / Enzymatic control of chitosan gelation for delivery of periodontal ligament cells. In: Macromolecular bioscience. 2014 ; Vol. 14, No. 7. pp. 1004-1014.
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Yan, XZ, Nijhuis, AWG, Van Den Beucken, JJJP, Both, SK, Jansen, JA, Leeuwenburgh, SCG & Yang, F 2014, 'Enzymatic control of chitosan gelation for delivery of periodontal ligament cells' Macromolecular bioscience, vol. 14, no. 7, pp. 1004-1014. https://doi.org/10.1002/mabi.201400040

Enzymatic control of chitosan gelation for delivery of periodontal ligament cells. / Yan, Xiang Zhen; Nijhuis, Arnold W.G.; Van Den Beucken, Jeroen J.J.P.; Both, Sanne K.; Jansen, John A.; Leeuwenburgh, Sander C.G.; Yang, Fang.

In: Macromolecular bioscience, Vol. 14, No. 7, 01.01.2014, p. 1004-1014.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Nijhuis, Arnold W.G.

AU - Van Den Beucken, Jeroen J.J.P.

AU - Both, Sanne K.

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AU - Leeuwenburgh, Sander C.G.

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Yan XZ, Nijhuis AWG, Van Den Beucken JJJP, Both SK, Jansen JA, Leeuwenburgh SCG et al. Enzymatic control of chitosan gelation for delivery of periodontal ligament cells. Macromolecular bioscience. 2014 Jan 1;14(7):1004-1014. https://doi.org/10.1002/mabi.201400040