Enzymes containing porous polymersomes as nano reaction vessels for cascade reactions

S. Kuiper; Madhavan Nallani; D.M. Vriezema; Jeroen Johannes Lambertus Maria Cornelissen; Jan van Hest; Roeland Nolte; A.E. Rowan

doi:10.1039/B811196K

Enzymes containing porous polymersomes as nano reaction vessels for cascade reactions

S. Kuiper, Madhavan Nallani, D.M. Vriezema, Jeroen Johannes Lambertus Maria Cornelissen, Jan van Hest, Roeland Nolte, A.E. Rowan

Biomolecular Nanotechnology

Research output: Contribution to journal › Article › Academic › peer-review

122 Citations (Scopus)

Abstract

Polystyrene40-b-poly(isocyanoalanine(2-thiophen-3-yl-ethyl)amide)50 (PS-PIAT) polymersomes have the unique property of being sufficiently porous to allow diffusion of small (organic) substrates while retaining large biomolecules such as enzymes inside. Herein we report on the encapsulation and protection of glucose oxidase (GOx) and horse radish peroxidase (HRP) in PS-PIAT polymersomes and the successful employment of these functionalised nanoreactors in a cascade reaction. The demonstrated concept allows for further application in other enzymatic cascade reactions, bio-organic hybrid systems and biosensing devices.

Original language	Undefined
Pages (from-to)	4315-4318
Journal	Organic & biomolecular chemistry
Volume	6
DOIs	https://doi.org/10.1039/B811196K
Publication status	Published - 2008

Keywords

METIS-306511

Access to Document

10.1039/B811196K

Cite this

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abstract = "Polystyrene40-b-poly(isocyanoalanine(2-thiophen-3-yl-ethyl)amide)50 (PS-PIAT) polymersomes have the unique property of being sufficiently porous to allow diffusion of small (organic) substrates while retaining large biomolecules such as enzymes inside. Herein we report on the encapsulation and protection of glucose oxidase (GOx) and horse radish peroxidase (HRP) in PS-PIAT polymersomes and the successful employment of these functionalised nanoreactors in a cascade reaction. The demonstrated concept allows for further application in other enzymatic cascade reactions, bio-organic hybrid systems and biosensing devices.",

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T1 - Enzymes containing porous polymersomes as nano reaction vessels for cascade reactions

AU - Kuiper, S.

AU - Nallani, Madhavan

AU - Vriezema, D.M.

AU - Cornelissen, Jeroen Johannes Lambertus Maria

AU - van Hest, Jan

AU - Nolte, Roeland

AU - Rowan, A.E.

PY - 2008

Y1 - 2008

N2 - Polystyrene40-b-poly(isocyanoalanine(2-thiophen-3-yl-ethyl)amide)50 (PS-PIAT) polymersomes have the unique property of being sufficiently porous to allow diffusion of small (organic) substrates while retaining large biomolecules such as enzymes inside. Herein we report on the encapsulation and protection of glucose oxidase (GOx) and horse radish peroxidase (HRP) in PS-PIAT polymersomes and the successful employment of these functionalised nanoreactors in a cascade reaction. The demonstrated concept allows for further application in other enzymatic cascade reactions, bio-organic hybrid systems and biosensing devices.

AB - Polystyrene40-b-poly(isocyanoalanine(2-thiophen-3-yl-ethyl)amide)50 (PS-PIAT) polymersomes have the unique property of being sufficiently porous to allow diffusion of small (organic) substrates while retaining large biomolecules such as enzymes inside. Herein we report on the encapsulation and protection of glucose oxidase (GOx) and horse radish peroxidase (HRP) in PS-PIAT polymersomes and the successful employment of these functionalised nanoreactors in a cascade reaction. The demonstrated concept allows for further application in other enzymatic cascade reactions, bio-organic hybrid systems and biosensing devices.

KW - METIS-306511

U2 - 10.1039/B811196K

DO - 10.1039/B811196K

M3 - Article

SN - 1477-0520

VL - 6

SP - 4315

EP - 4318

JO - Organic & biomolecular chemistry

JF - Organic & biomolecular chemistry

ER -