TY - JOUR
T1 - Evaluation of the inflammatory responses to sol–gel coatings with distinct biocompatibility levels
AU - Cerqueira, Andreia
AU - Araújo-Gomes, Nuno
AU - Zhang, Yang
AU - van den Beucken, Jeroen J.J.P.
AU - Martínez-Ramos, Cristina
AU - Ozturan, Seda
AU - Izquierdo, Raúl
AU - Muriach, María
AU - Romero-Cano, Ricard
AU - Baliño, Pablo
AU - Romero-Gavilán, Francisco J.
N1 - Funding Information:
This work was supported by MINECO [MAT2017-86043-R; RTC-2017-6147-1]; Universitat Jaume I [POSDOC/2019/28] and Generalitat Valenciana [GRISOLIAP/2018/091]. Authors would like to thank Antonio Coso (GMI-Ilerimplant) for their inestimable contribution to this study, and Raquel Oliver and Jose Ortega for their valuable technical assistance.
Funding Information:
This work was supported by MINECO [MAT2017‐86043‐R; RTC‐2017‐6147‐1]; Universitat Jaume I [POSDOC/2019/28] and Generalitat Valenciana [GRISOLIAP/2018/091]. Authors would like to thank Antonio Coso (GMI‐Ilerimplant) for their inestimable contribution to this study, and Raquel Oliver and Jose Ortega for their valuable technical assistance.
Publisher Copyright:
© 2021 Wiley Periodicals LLC
PY - 2021/9
Y1 - 2021/9
N2 - The immune system plays a crucial role in determining the implantation outcome, and macrophages are in the frontline of the inflammatory processes. Further, cellular oxidative stress resulting from the material recognition can influence how cell responses develop. Considering this, the aim of this study was to study oxidative stress and macrophages phenotypes in response to sol–gel materials with distinct in vivo outcomes. Four materials were selected (70M30T and 35M35G30T, with high biocompatibility, and 50M50G and 50V50G, with low biocompatibility). Gene expression, immunocytochemistry and cytokine secretion profiles for M1 and M2 markers were determined. Moreover, oxidative stress markers were studied. Immunocytochemistry and ELISA showed that 50M50G and 50V50G lead to a higher differentiation to M1 phenotype, while 70M30T and 35M35G30T promoted M2 differentiation. In oxidative stress, no differences were found. These results show that the balance between M1 and M2, more than individual quantification of each phenotype, determines a biomaterial outcome.
AB - The immune system plays a crucial role in determining the implantation outcome, and macrophages are in the frontline of the inflammatory processes. Further, cellular oxidative stress resulting from the material recognition can influence how cell responses develop. Considering this, the aim of this study was to study oxidative stress and macrophages phenotypes in response to sol–gel materials with distinct in vivo outcomes. Four materials were selected (70M30T and 35M35G30T, with high biocompatibility, and 50M50G and 50V50G, with low biocompatibility). Gene expression, immunocytochemistry and cytokine secretion profiles for M1 and M2 markers were determined. Moreover, oxidative stress markers were studied. Immunocytochemistry and ELISA showed that 50M50G and 50V50G lead to a higher differentiation to M1 phenotype, while 70M30T and 35M35G30T promoted M2 differentiation. In oxidative stress, no differences were found. These results show that the balance between M1 and M2, more than individual quantification of each phenotype, determines a biomaterial outcome.
KW - 2022 OA procedure
KW - implants
KW - inflammation
KW - macrophage plasticity
KW - oxidative markers
KW - biomaterials
UR - http://www.scopus.com/inward/record.url?scp=85101158464&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.37149
DO - 10.1002/jbm.a.37149
M3 - Article
AN - SCOPUS:85101158464
SN - 1549-3296
VL - 109
SP - 1539
EP - 1548
JO - Journal of biomedical materials research. Part A
JF - Journal of biomedical materials research. Part A
IS - 9
ER -