Evaluation of the viability of HL60 cells in contact with commonly used microchip materials

F. Wolbers, Paulus Martinus ter Braak, Severine le Gac, Regina Lüttge, Helene Andersson, I. Vermes, Albert van den Berg

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Abstract

This paper presents beneficial data when deciding to perform cell experiments in lab-on- a-chip devices. The choice of material can influence the viability of mammalian cells. PDMS, precoated with serum or not, suits well for HL60 cells, demonstrating the best results in the viability experiments, as expressed as an apoptosis/proliferation ratio < 1. We also looked at other materials, such as native silicon-oxide (SiO2) and borosilicate glass. In the near future other (combinations) of microchip materials will be analyzed for their viability as well as analyzing the effect of mammalian cells on microchip materials in more detail with MALDI-MS.
Original languageUndefined
Title of host publicationMicroTAS 2005 Conference - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
EditorsK.F Jensen, J. Han, D.J. Harrison, J. Voldman
Place of PublicationSan Diego, CA, USA
PublisherTransducer Research Foundation, Inc.
Pages1288-1290
Number of pages3
ISBN (Print)9780444511003
Publication statusPublished - 9 Oct 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005 - Boston, United States
Duration: 9 Oct 200513 Oct 2005
Conference number: 9

Publication series

Name
PublisherTransducer Research Foundation, Inc.
Volume2

Conference

Conference9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005
Abbreviated titleMicroTAS
CountryUnited States
CityBoston
Period9/10/0513/10/05

Keywords

  • METIS-224696
  • IR-52868
  • EWI-18779
  • Viability
  • HL60 cells
  • Lab on a chip

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