Evaluation of the viability of HL60 cells in contact with commonly used microchip materials

F. Wolbers; Paulus Martinus ter Braak; Severine le Gac; Regina Lüttge; Helene Andersson; I. Vermes; Albert van den Berg

Evaluation of the viability of HL60 cells in contact with commonly used microchip materials

F. Wolbers, Paulus Martinus ter Braak, Severine le Gac, Regina Lüttge, Helene Andersson, I. Vermes, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

This paper presents beneficial data when deciding to perform cell experiments in lab-on- a-chip devices. The choice of material can influence the viability of mammalian cells. PDMS, precoated with serum or not, suits well for HL60 cells, demonstrating the best results in the viability experiments, as expressed as an apoptosis/proliferation ratio < 1. We also looked at other materials, such as native silicon-oxide (SiO2) and borosilicate glass. In the near future other (combinations) of microchip materials will be analyzed for their viability as well as analyzing the effect of mammalian cells on microchip materials in more detail with MALDI-MS.

Original language	Undefined
Title of host publication	MicroTAS 2005 Conference - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Editors	K.F Jensen, J. Han, D.J. Harrison, J. Voldman
Place of Publication	San Diego, CA, USA
Publisher	Transducer Research Foundation, Inc.
Pages	1288-1290
Number of pages	3
ISBN (Print)	9780444511003
Publication status	Published - 9 Oct 2005
Event	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005 - Boston, United States Duration: 9 Oct 2005 → 13 Oct 2005 Conference number: 9

Publication series

Name
Publisher	Transducer Research Foundation, Inc.
Volume	2

Conference

Conference	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005
Abbreviated title	MicroTAS
Country/Territory	United States
City	Boston
Period	9/10/05 → 13/10/05

Keywords

METIS-224696
IR-52868
EWI-18779
Viability
HL60 cells
Lab on a chip

Access to Document

wolbers-MicroTAS2005

Cite this

Wolbers, F., ter Braak, P. M., le Gac, S., Lüttge, R., Andersson, H., Vermes, I., & van den Berg, A. (2005). Evaluation of the viability of HL60 cells in contact with commonly used microchip materials. In K. F. Jensen, J. Han, D. J. Harrison, & J. Voldman (Eds.), MicroTAS 2005 Conference - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1288-1290). Transducer Research Foundation, Inc..

Wolbers, F. ; ter Braak, Paulus Martinus ; le Gac, Severine et al. / Evaluation of the viability of HL60 cells in contact with commonly used microchip materials. MicroTAS 2005 Conference - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. editor / K.F Jensen ; J. Han ; D.J. Harrison ; J. Voldman. San Diego, CA, USA : Transducer Research Foundation, Inc., 2005. pp. 1288-1290

@inproceedings{820eb59422f84aacac1347e345c6bfb1,

title = "Evaluation of the viability of HL60 cells in contact with commonly used microchip materials",

abstract = "This paper presents beneficial data when deciding to perform cell experiments in lab-on- a-chip devices. The choice of material can influence the viability of mammalian cells. PDMS, precoated with serum or not, suits well for HL60 cells, demonstrating the best results in the viability experiments, as expressed as an apoptosis/proliferation ratio < 1. We also looked at other materials, such as native silicon-oxide (SiO2) and borosilicate glass. In the near future other (combinations) of microchip materials will be analyzed for their viability as well as analyzing the effect of mammalian cells on microchip materials in more detail with MALDI-MS.",

keywords = "METIS-224696, IR-52868, EWI-18779, Viability, HL60 cells, Lab on a chip",

author = "F. Wolbers and {ter Braak}, {Paulus Martinus} and {le Gac}, Severine and Regina L{\"u}ttge and Helene Andersson and I. Vermes and {van den Berg}, Albert",

year = "2005",

month = oct,

day = "9",

language = "Undefined",

isbn = "9780444511003",

publisher = "Transducer Research Foundation, Inc.",

pages = "1288--1290",

editor = "K.F Jensen and J. Han and D.J. Harrison and J. Voldman",

booktitle = "MicroTAS 2005 Conference - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

note = "null ; Conference date: 09-10-2005 Through 13-10-2005",

}

Wolbers, F, ter Braak, PM , le Gac, S, Lüttge, R, Andersson, H, Vermes, I & van den Berg, A 2005, Evaluation of the viability of HL60 cells in contact with commonly used microchip materials. in KF Jensen, J Han, DJ Harrison & J Voldman (eds), MicroTAS 2005 Conference - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation, Inc., San Diego, CA, USA, pp. 1288-1290, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005, Boston, Massachusetts, United States, 9/10/05.

Evaluation of the viability of HL60 cells in contact with commonly used microchip materials. / Wolbers, F.; ter Braak, Paulus Martinus ; le Gac, Severine et al.

MicroTAS 2005 Conference - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. ed. / K.F Jensen; J. Han; D.J. Harrison; J. Voldman. San Diego, CA, USA : Transducer Research Foundation, Inc., 2005. p. 1288-1290.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Evaluation of the viability of HL60 cells in contact with commonly used microchip materials

AU - Wolbers, F.

AU - ter Braak, Paulus Martinus

AU - le Gac, Severine

AU - Lüttge, Regina

AU - Andersson, Helene

AU - Vermes, I.

AU - van den Berg, Albert

N1 - Conference code: 9

PY - 2005/10/9

Y1 - 2005/10/9

N2 - This paper presents beneficial data when deciding to perform cell experiments in lab-on- a-chip devices. The choice of material can influence the viability of mammalian cells. PDMS, precoated with serum or not, suits well for HL60 cells, demonstrating the best results in the viability experiments, as expressed as an apoptosis/proliferation ratio < 1. We also looked at other materials, such as native silicon-oxide (SiO2) and borosilicate glass. In the near future other (combinations) of microchip materials will be analyzed for their viability as well as analyzing the effect of mammalian cells on microchip materials in more detail with MALDI-MS.

AB - This paper presents beneficial data when deciding to perform cell experiments in lab-on- a-chip devices. The choice of material can influence the viability of mammalian cells. PDMS, precoated with serum or not, suits well for HL60 cells, demonstrating the best results in the viability experiments, as expressed as an apoptosis/proliferation ratio < 1. We also looked at other materials, such as native silicon-oxide (SiO2) and borosilicate glass. In the near future other (combinations) of microchip materials will be analyzed for their viability as well as analyzing the effect of mammalian cells on microchip materials in more detail with MALDI-MS.

KW - METIS-224696

KW - IR-52868

KW - EWI-18779

KW - Viability

KW - HL60 cells

KW - Lab on a chip

M3 - Conference contribution

SN - 9780444511003

SP - 1288

EP - 1290

BT - MicroTAS 2005 Conference - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences

A2 - Jensen, K.F

A2 - Han, J.

A2 - Harrison, D.J.

A2 - Voldman, J.

PB - Transducer Research Foundation, Inc.

CY - San Diego, CA, USA

Y2 - 9 October 2005 through 13 October 2005

ER -

Wolbers F, ter Braak PM , le Gac S, Lüttge R, Andersson H, Vermes I et al. Evaluation of the viability of HL60 cells in contact with commonly used microchip materials. In Jensen KF, Han J, Harrison DJ, Voldman J, editors, MicroTAS 2005 Conference - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. San Diego, CA, USA: Transducer Research Foundation, Inc. 2005. p. 1288-1290