Expansion of bovine chondrocytes on microcarriers enhances redifferentiation

J. Malda, Clemens van Blitterswijk, M. Grojec, D.E. Martens, J. Tramper, J.U. Riesle

Research output: Contribution to journalArticleAcademicpeer-review

106 Citations (Scopus)

Abstract

Functional cartilage implants for orthopedic surgery or in vitro tissue evaluation can be created from expanded chondrocytes and biodegradable scaffolds. Expansion of chondrocytes in two-dimensional culture systems results in their dedifferentiation. The hallmark of this process is the switch of collagen synthesis from type II to type I. The aim of this study was to evaluate the postexpansion chondrogenic potential of microcarrier-expanded bovine articular chondrocytes in pellet cultures. A selection of microcarriers was screened for initial attachment of chondrocytes. On the basis of those results and additional selection criteria related to clinical application, Cytodex-1 microcarriers were selected for further investigation. Comparable doubling times were obtained in T-flask and microcarrier cultures. During propagation on Cytodex-1 microcarriers, cells acquired a spherical-like morphology and the presence of collagen type II was detected. Both observations are indicative of a differentiated chondrocyte. Pellet cultures of microcarrier-expanded cells showed cartilage-like morphology and staining for proteoglycans and collagen type II after 14 days. In contrast, pellets of T-flask-expanded cells had a fibrous appearance and showed abundant staining only for collagen type I. Therefore, culture of chondrocytes on microcarriers may offer useful and cost-effective cell expansion opportunities in the field of cartilage tissue engineering.
Original languageUndefined
Pages (from-to)949-956
JournalTissue engineering
Volume9
Issue number5
DOIs
Publication statusPublished - 2003

Keywords

  • METIS-216592
  • IR-67212

Cite this

Malda, J., van Blitterswijk, C., Grojec, M., Martens, D. E., Tramper, J., & Riesle, J. U. (2003). Expansion of bovine chondrocytes on microcarriers enhances redifferentiation. Tissue engineering, 9(5), 949-956. https://doi.org/10.1089/107632703322495583
Malda, J. ; van Blitterswijk, Clemens ; Grojec, M. ; Martens, D.E. ; Tramper, J. ; Riesle, J.U. / Expansion of bovine chondrocytes on microcarriers enhances redifferentiation. In: Tissue engineering. 2003 ; Vol. 9, No. 5. pp. 949-956.
@article{cf835307b8b04f85a5578c166545b485,
title = "Expansion of bovine chondrocytes on microcarriers enhances redifferentiation",
abstract = "Functional cartilage implants for orthopedic surgery or in vitro tissue evaluation can be created from expanded chondrocytes and biodegradable scaffolds. Expansion of chondrocytes in two-dimensional culture systems results in their dedifferentiation. The hallmark of this process is the switch of collagen synthesis from type II to type I. The aim of this study was to evaluate the postexpansion chondrogenic potential of microcarrier-expanded bovine articular chondrocytes in pellet cultures. A selection of microcarriers was screened for initial attachment of chondrocytes. On the basis of those results and additional selection criteria related to clinical application, Cytodex-1 microcarriers were selected for further investigation. Comparable doubling times were obtained in T-flask and microcarrier cultures. During propagation on Cytodex-1 microcarriers, cells acquired a spherical-like morphology and the presence of collagen type II was detected. Both observations are indicative of a differentiated chondrocyte. Pellet cultures of microcarrier-expanded cells showed cartilage-like morphology and staining for proteoglycans and collagen type II after 14 days. In contrast, pellets of T-flask-expanded cells had a fibrous appearance and showed abundant staining only for collagen type I. Therefore, culture of chondrocytes on microcarriers may offer useful and cost-effective cell expansion opportunities in the field of cartilage tissue engineering.",
keywords = "METIS-216592, IR-67212",
author = "J. Malda and {van Blitterswijk}, Clemens and M. Grojec and D.E. Martens and J. Tramper and J.U. Riesle",
year = "2003",
doi = "10.1089/107632703322495583",
language = "Undefined",
volume = "9",
pages = "949--956",
journal = "Tissue engineering",
issn = "1076-3279",
publisher = "Mary Ann Liebert Inc.",
number = "5",

}

Malda, J, van Blitterswijk, C, Grojec, M, Martens, DE, Tramper, J & Riesle, JU 2003, 'Expansion of bovine chondrocytes on microcarriers enhances redifferentiation' Tissue engineering, vol. 9, no. 5, pp. 949-956. https://doi.org/10.1089/107632703322495583

Expansion of bovine chondrocytes on microcarriers enhances redifferentiation. / Malda, J.; van Blitterswijk, Clemens; Grojec, M.; Martens, D.E.; Tramper, J.; Riesle, J.U.

In: Tissue engineering, Vol. 9, No. 5, 2003, p. 949-956.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Expansion of bovine chondrocytes on microcarriers enhances redifferentiation

AU - Malda, J.

AU - van Blitterswijk, Clemens

AU - Grojec, M.

AU - Martens, D.E.

AU - Tramper, J.

AU - Riesle, J.U.

PY - 2003

Y1 - 2003

N2 - Functional cartilage implants for orthopedic surgery or in vitro tissue evaluation can be created from expanded chondrocytes and biodegradable scaffolds. Expansion of chondrocytes in two-dimensional culture systems results in their dedifferentiation. The hallmark of this process is the switch of collagen synthesis from type II to type I. The aim of this study was to evaluate the postexpansion chondrogenic potential of microcarrier-expanded bovine articular chondrocytes in pellet cultures. A selection of microcarriers was screened for initial attachment of chondrocytes. On the basis of those results and additional selection criteria related to clinical application, Cytodex-1 microcarriers were selected for further investigation. Comparable doubling times were obtained in T-flask and microcarrier cultures. During propagation on Cytodex-1 microcarriers, cells acquired a spherical-like morphology and the presence of collagen type II was detected. Both observations are indicative of a differentiated chondrocyte. Pellet cultures of microcarrier-expanded cells showed cartilage-like morphology and staining for proteoglycans and collagen type II after 14 days. In contrast, pellets of T-flask-expanded cells had a fibrous appearance and showed abundant staining only for collagen type I. Therefore, culture of chondrocytes on microcarriers may offer useful and cost-effective cell expansion opportunities in the field of cartilage tissue engineering.

AB - Functional cartilage implants for orthopedic surgery or in vitro tissue evaluation can be created from expanded chondrocytes and biodegradable scaffolds. Expansion of chondrocytes in two-dimensional culture systems results in their dedifferentiation. The hallmark of this process is the switch of collagen synthesis from type II to type I. The aim of this study was to evaluate the postexpansion chondrogenic potential of microcarrier-expanded bovine articular chondrocytes in pellet cultures. A selection of microcarriers was screened for initial attachment of chondrocytes. On the basis of those results and additional selection criteria related to clinical application, Cytodex-1 microcarriers were selected for further investigation. Comparable doubling times were obtained in T-flask and microcarrier cultures. During propagation on Cytodex-1 microcarriers, cells acquired a spherical-like morphology and the presence of collagen type II was detected. Both observations are indicative of a differentiated chondrocyte. Pellet cultures of microcarrier-expanded cells showed cartilage-like morphology and staining for proteoglycans and collagen type II after 14 days. In contrast, pellets of T-flask-expanded cells had a fibrous appearance and showed abundant staining only for collagen type I. Therefore, culture of chondrocytes on microcarriers may offer useful and cost-effective cell expansion opportunities in the field of cartilage tissue engineering.

KW - METIS-216592

KW - IR-67212

U2 - 10.1089/107632703322495583

DO - 10.1089/107632703322495583

M3 - Article

VL - 9

SP - 949

EP - 956

JO - Tissue engineering

JF - Tissue engineering

SN - 1076-3279

IS - 5

ER -

Malda J, van Blitterswijk C, Grojec M, Martens DE, Tramper J, Riesle JU. Expansion of bovine chondrocytes on microcarriers enhances redifferentiation. Tissue engineering. 2003;9(5):949-956. https://doi.org/10.1089/107632703322495583