Exploiting speckle correlations to improve the resolution of wide-field fluorescence microscopy

H. Yilmaz, E.G. van Putten, J. Bertolotti, Aart Lagendijk, Willem L. Vos, Allard Mosk

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Abstract

Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field of view. Due to the diffraction limit, conventional optical microscopes are limited to resolving structures larger than 200 nm. The resolution can be enhanced by near-field and far-field super-resolution microscopy methods. Near-field methods typically have a limited field of view and far-field methods are limited by the involved conventional optics. Here, we introduce a combined high-resolution and wide-field fluorescence microscopy method that improves the resolution of a conventional optical microscope by exploiting correlations in speckle illumination through a randomly scattering high-index medium: Speckle correlation resolution enhancement (SCORE). As a test, we collect two-dimensional fluorescence images of 100-nm diameter dye-doped nanospheres. We demonstrate a deconvolved resolution of 130 nm with a field of view of 10 x 10 \mu m 2 .
Original languageEnglish
Publication statusPublished - 2014

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microscopy
fluorescence
field of view
optical microscopes
far fields
near fields
dyes
versatility
marking
illumination
optics
augmentation
high resolution
scattering
diffraction

Keywords

  • IR-95139
  • METIS-309365

Cite this

Yilmaz, H. (Author), van Putten, E. G. (Author), Bertolotti, J. (Author), Lagendijk, A. (Author), Vos, W. L. (Author), & Mosk, A. (Author). (2014). Exploiting speckle correlations to improve the resolution of wide-field fluorescence microscopy. Web publication/site, Retrieved from http://arxiv.org/abs/1410.2079
Yilmaz, H. (Author) ; van Putten, E.G. (Author) ; Bertolotti, J. (Author) ; Lagendijk, Aart (Author) ; Vos, Willem L. (Author) ; Mosk, Allard (Author). / Exploiting speckle correlations to improve the resolution of wide-field fluorescence microscopy. [Web publication/site].
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Yilmaz, H, van Putten, EG, Bertolotti, J, Lagendijk, A, Vos, WL & Mosk, A, Exploiting speckle correlations to improve the resolution of wide-field fluorescence microscopy, 2014, Web publication/site.
Exploiting speckle correlations to improve the resolution of wide-field fluorescence microscopy. Yilmaz, H. (Author); van Putten, E.G. (Author); Bertolotti, J. (Author); Lagendijk, Aart (Author); Vos, Willem L. (Author); Mosk, Allard (Author). 2014.

Research output: Non-textual formWeb publication/siteOther research output

TY - ADVS

T1 - Exploiting speckle correlations to improve the resolution of wide-field fluorescence microscopy

AU - Yilmaz, H.

AU - van Putten, E.G.

AU - Bertolotti, J.

AU - Lagendijk, Aart

AU - Vos, Willem L.

AU - Mosk, Allard

PY - 2014

Y1 - 2014

N2 - Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field of view. Due to the diffraction limit, conventional optical microscopes are limited to resolving structures larger than 200 nm. The resolution can be enhanced by near-field and far-field super-resolution microscopy methods. Near-field methods typically have a limited field of view and far-field methods are limited by the involved conventional optics. Here, we introduce a combined high-resolution and wide-field fluorescence microscopy method that improves the resolution of a conventional optical microscope by exploiting correlations in speckle illumination through a randomly scattering high-index medium: Speckle correlation resolution enhancement (SCORE). As a test, we collect two-dimensional fluorescence images of 100-nm diameter dye-doped nanospheres. We demonstrate a deconvolved resolution of 130 nm with a field of view of 10 x 10 \mu m 2 .

AB - Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field of view. Due to the diffraction limit, conventional optical microscopes are limited to resolving structures larger than 200 nm. The resolution can be enhanced by near-field and far-field super-resolution microscopy methods. Near-field methods typically have a limited field of view and far-field methods are limited by the involved conventional optics. Here, we introduce a combined high-resolution and wide-field fluorescence microscopy method that improves the resolution of a conventional optical microscope by exploiting correlations in speckle illumination through a randomly scattering high-index medium: Speckle correlation resolution enhancement (SCORE). As a test, we collect two-dimensional fluorescence images of 100-nm diameter dye-doped nanospheres. We demonstrate a deconvolved resolution of 130 nm with a field of view of 10 x 10 \mu m 2 .

KW - IR-95139

KW - METIS-309365

M3 - Web publication/site

ER -