TY - JOUR
T1 - Expression of inflammation in myocardial tissue of rabbits
T2 - Comparison of two miniaturized heart-lung machines
AU - Schnoering, Heike
AU - Arens, Jutta
AU - Detering, Sabine M.
AU - Sachweh, Joerg S.
AU - Goetzenich, Andreas
AU - Schmitz-Rode, Thomas
AU - Steinseifer, Ulrich
AU - Vazquez-Jimenez, Jaime F.
PY - 2013/6/1
Y1 - 2013/6/1
N2 - The majority of cases involving the surgical treatment of congenital heart disease require implementation of cardiopulmonary bypass (CPB). However, neonates and infants are particularly prone to serious complications associated with CPB as a result of capillary leak due to cardiovascular failure. These complications are related to the transfusion of foreign blood, the disproportionately large area of contact between the patient's blood and foreign material, as well as the systemic inflammatory response induced by hemolysis. To attenuate these risks, we developed a novel, highly integrative, miniaturized heart-lung machine (MiniHLM) with a static priming volume of only 102mL. This prototype was tested in comparison with a conventional heart-lung machine (static priming volume 213mL) using a rabbit animal model. The animals were anesthetized, sternotomized, and connected to CBP via the aorta and right atrium. The aorta was cross-clamped for 1h. Blood samples for examination were taken at regular intervals. Biopsies of the right atrial appendage (RAA) were removed directly after initiation and after cessation of CPB. After gradual reduction of perfusion with the HLM, all rabbits were successfully weaned from CPB, and the sternum was closed. Foreign blood was not administered in all cases. After cryopreservation of the RAA tissue, de novo transcription of inflammatory cytokines was measured by means of real-time polymerase chain reaction using the comparative CT method. No significant differences in the expression of the inflammatory parameters of the myocardial tissue samples were found between the study groups.
AB - The majority of cases involving the surgical treatment of congenital heart disease require implementation of cardiopulmonary bypass (CPB). However, neonates and infants are particularly prone to serious complications associated with CPB as a result of capillary leak due to cardiovascular failure. These complications are related to the transfusion of foreign blood, the disproportionately large area of contact between the patient's blood and foreign material, as well as the systemic inflammatory response induced by hemolysis. To attenuate these risks, we developed a novel, highly integrative, miniaturized heart-lung machine (MiniHLM) with a static priming volume of only 102mL. This prototype was tested in comparison with a conventional heart-lung machine (static priming volume 213mL) using a rabbit animal model. The animals were anesthetized, sternotomized, and connected to CBP via the aorta and right atrium. The aorta was cross-clamped for 1h. Blood samples for examination were taken at regular intervals. Biopsies of the right atrial appendage (RAA) were removed directly after initiation and after cessation of CPB. After gradual reduction of perfusion with the HLM, all rabbits were successfully weaned from CPB, and the sternum was closed. Foreign blood was not administered in all cases. After cryopreservation of the RAA tissue, de novo transcription of inflammatory cytokines was measured by means of real-time polymerase chain reaction using the comparative CT method. No significant differences in the expression of the inflammatory parameters of the myocardial tissue samples were found between the study groups.
KW - Animal experimentation
KW - Cardiopulmonary bypass
KW - Heart biopsy
KW - Heart-lung machine
KW - Inflammation
UR - http://www.scopus.com/inward/record.url?scp=84879104655&partnerID=8YFLogxK
U2 - 10.1111/aor.12025
DO - 10.1111/aor.12025
M3 - Article
C2 - 23578310
AN - SCOPUS:84879104655
VL - 37
SP - 541
EP - 548
JO - Artificial organs
JF - Artificial organs
SN - 0160-564X
IS - 6
ER -