Extending the microfabrication and particle handling toolbox for the development of ordered chromatography columns

Sandrien Verloy

Research output: ThesisPhD Thesis - Research external, graduation UT

91 Downloads (Pure)

Abstract

Improving the order of the beads packed in a liquid chromatography column would practically translate into a situation where for instance a researcher in the biomedical field analyzing a biological sample (e.g. a cancer cell) would be able to identify a multifold more molecules (eg proteins), using one and the same, yet optimized liquid chromatography column.

The present work investigates a number of new techniques to produce order columns suitable for chromatography. These techniques include a combination of deep reactive ion etching in silicon with mechanical rubbing and other particle handling methods to fill arrays of micro-pockets with either single particles or a well-defined multiple. A broad range of particle handling methods has been explored and has led to a fully optimized procedure involving a novel combination of micro-structured polydimethylsiloxane and wet manual rubbing. During the work, the power of silicon micromachining has also been explored to produce perfectly ordered columns for gas chromatography.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • University of Twente
Supervisors/Advisors
  • Desmet, Gert, Supervisor, External person
  • Gardeniers, J.G.E., Supervisor
Thesis sponsors
Award date2 Jun 2022
Place of PublicationEnschede
Publisher
Print ISBNs978-90-365-5390-2
DOIs
Publication statusPublished - 2 Jun 2022

Keywords

  • Microfluidic devices
  • Ordered particle arrays
  • Particle assembly
  • PDMS rubbing
  • Microfabrication
  • Chromatography
  • Particle deposition
  • Particle packing

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