Extracellular detection of active membrane currents in the neuron-electrode interface

J.R. Buitenweg, W.L.C. Rutten, E. Marani, S.K.L. Polman, J. Ursum

    Research output: Contribution to journalArticleAcademicpeer-review

    17 Citations (Scopus)

    Abstract

    Although measurement of sealing resistance is an important tool in the assessment of the electrical contacts between cultured cells and substrate embedded microelectrodes, it does not offer information about the type of cell, i.e. neuron or non-neuronal cell. Also, rules for translation of a measured sealing resistance into parameters for successful stimulation, i.e. eliciting an action potential, are not available yet. Therefore, a method is proposed for the detection of active membrane currents, elicited by extracellular current stimulation. The method is based on the prediction of the linear part of the response to an applied stimulus current pulse using an impedance model of the neuron–electrode contact. Active membrane currents are detected in the nonlinear response, which is obtained by subtraction of the predicted linear response from the measured response. The required impedance model parameters are extracted from impedance spectroscopy or directly from the measured responses.
    Original languageEnglish
    Pages (from-to)211-221
    Number of pages11
    JournalJournal of neuroscience methods
    Volume115
    Issue number2
    DOIs
    Publication statusPublished - 2002

    Keywords

    • Impedance spectroscopy
    • Neuron–electrode contact
    • Extracellular stimulation
    • Cultured neurons
    • Sealing resistance
    • Microelectrode arrays

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