@article{3d5747f921a543f2a13b80e48328581a,
title = "Extracellular-Matrix-Reinforced Bioinks for 3D Bioprinting Human Tissue",
abstract = "Recent advances in 3D bioprinting allow for generating intricate structures with dimensions relevant for human tissue, but suitable bioinks for producing translationally relevant tissue with complex geometries remain unidentified. Here, a tissue-specific hybrid bioink is described, composed of a natural polymer, alginate, reinforced with extracellular matrix derived from decellularized tissue (rECM). rECM has rheological and gelation properties beneficial for 3D bioprinting while retaining biologically inductive properties supporting tissue maturation ex vivo and in vivo. These bioinks are shear thinning, resist cell sedimentation, improve viability of multiple cell types, and enhance mechanical stability in hydrogels derived from them. 3D printed constructs generated from rECM bioinks suppress the foreign body response, are pro-angiogenic and support recipient-derived de novo blood vessel formation across the entire graft thickness in a murine model of transplant immunosuppression. Their proof-of-principle for generating human tissue is demonstrated by 3D bioprinting human airways composed of regionally specified primary human airway epithelial progenitor and smooth muscle cells. Airway lumens remained patent with viable cells for one month in vitro with evidence of differentiation into mature epithelial cell types found in native human airways. rECM bioinks are a promising new approach for generating functional human tissue using 3D bioprinting.",
keywords = "biofabrication, bioinks, extracellular matrix, tissue engineering, 3D bioprinting",
author = "{De Santis}, {Martina M.} and Alsafadi, {Hani N.} and Sinem Tas and B{\"o}l{\"u}kbas, {Deniz A.} and Sujeethkumar Prithiviraj and {Da Silva}, {Iran A.N.} and Margareta Mittendorfer and Chiharu Ota and John Stegmayr and Fatima Daoud and Melanie K{\"o}nigshoff and Karl Sw{\"a}rd and Wood, {Jeffery A.} and Manlio Tassieri and Bourgine, {Paul E.} and Sandra Lindstedt and Sofie Mohlin and Wagner, {Darcy E.}",
note = "Funding Information: The authors thank Adam Feinberg, Ramon Farr?, and Jordi Otero D?az for helpful discussions on 3D bioprinting and mechanical characterization. The authors are grateful to Rita Costa, Ali Doryab, Hanna Thorsson, and Oskar Hallgren for their insights. The authors thank Sebastian Wasserstrom from LBIC for technical assistance with the SEM and confocal microscopy, Bengt Mattsson for assistance with light sheet microscopy, Anja Meissner for providing the bEnd.3 cell line and mice, Anki Knutsson, Kinga I. Gawlik, and Madeleine Durbeej-Hjalt for mice, Hakon Leffler for use of the PHERAstar FS spectrometer, and Luigi Gentile for assistance with the rheometer. This project has received funding from the European Research Council (ERC) (grant agreement No 805361) (D.E.W.). Further support is acknowledged from the Knut and Alice Wallenberg foundation (D.E.W., S.L., and P.E.B.), German Lung Center (M.K. and D.E.W.), a Helmholtz Munich Postdoctoral Fellowship (D.E.W.), and grants granted by the Swedish Childhood Cancer Foundation and the Swedish Cancer Society (S.M.). S.T. is supported by a RESPIRE3 Postdoctoral Fellowship supported by the European Respiratory Society and the European Union's H2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement (Grant No. 713406). All animal studies were performed under the strict regulation of the Swedish board of agriculture and approved by the Malm?-Lund Animal Ethics Committee (Approval numbers: 5.8.18/12637/2017, M 152-14 and M 57-16). Human lung tissue was obtained from discarded surgical waste from donor lungs following lung transplantation. The study was approved by the Regional Ethics Review Board in Lund, Sweden (Dnr. 2017/396; Dnr 2018/386) and conducted in accordance with the Declaration of Helsinki with written informed consent from all patients and in accordance with the European Union General Data Protection Regulation (GDPR). Publisher Copyright: {\textcopyright} 2020 The Authors. Advanced Materials published by Wiley-VCH GmbH",
year = "2021",
month = jan,
day = "21",
doi = "10.1002/adma.202005476",
language = "English",
volume = "33",
journal = "Advanced materials",
issn = "0935-9648",
publisher = "Wiley-Blackwell",
number = "3",
}