Extraction of ginsenosides from North American ginseng using modified supercritical carbon dioxide

Jeffery A. Wood, Mark A. Bernards, Wan kei Wan, Paul A. Charpentier*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

56 Citations (Scopus)


The root of North American ginseng (Panax quinquefolius) was extracted using conventional solvent techniques (Soxhlet) and using supercritical fluid extraction (SFE). SFE was accomplished using modified supercritical carbon dioxide (scCO2), with methanol and DMSO as modifiers. At a temperature of 110 °C and a modifier to solid ratio of 4.1 g of modifier per gram of ginseng, the system pressure was found to have no significant effect on the quantity of ginsenosides extracted from 20.7 to 48.3 MPa using CO2 + methanol. In addition, the process was shown to be mass transfer/desorption limited rather than solubility limited under these conditions. For the studied SFE conditions, up to 90% of the total ginsenosides were extracted compared to conventional methanol Soxhlet, as determined by HPLC analysis. A relatively unknown ginsenoside peak was observed in the HPLC chromatograms for all supercritical extractions, particularly those employing DMSO as a modifier. This peak was identified as 6″/6″″-mono-O-acetyl ginsenoside Rb1 by LC/MS.

Original languageEnglish
Pages (from-to)40-47
Number of pages8
JournalJournal of supercritical fluids
Issue number1
Publication statusPublished - 1 Nov 2006
Externally publishedYes


  • Carbon dioxide
  • Extraction
  • Ginseng
  • Ginsenosides
  • Supercritical fluids


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