Abstract
The root of North American ginseng (Panax quinquefolius) was extracted using conventional solvent techniques (Soxhlet) and using supercritical fluid extraction (SFE). SFE was accomplished using modified supercritical carbon dioxide (scCO2), with methanol and DMSO as modifiers. At a temperature of 110 °C and a modifier to solid ratio of 4.1 g of modifier per gram of ginseng, the system pressure was found to have no significant effect on the quantity of ginsenosides extracted from 20.7 to 48.3 MPa using CO2 + methanol. In addition, the process was shown to be mass transfer/desorption limited rather than solubility limited under these conditions. For the studied SFE conditions, up to 90% of the total ginsenosides were extracted compared to conventional methanol Soxhlet, as determined by HPLC analysis. A relatively unknown ginsenoside peak was observed in the HPLC chromatograms for all supercritical extractions, particularly those employing DMSO as a modifier. This peak was identified as 6″/6″″-mono-O-acetyl ginsenoside Rb1 by LC/MS.
| Original language | English |
|---|---|
| Pages (from-to) | 40-47 |
| Number of pages | 8 |
| Journal | Journal of supercritical fluids |
| Volume | 39 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Nov 2006 |
| Externally published | Yes |
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Keywords
- Carbon dioxide
- Extraction
- Ginseng
- Ginsenosides
- Supercritical fluids
Cite this
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Extraction of ginsenosides from North American ginseng using modified supercritical carbon dioxide. / Wood, Jeffery A.; Bernards, Mark A.; Wan, Wan kei; Charpentier, Paul A.
In: Journal of supercritical fluids, Vol. 39, No. 1, 01.11.2006, p. 40-47.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Extraction of ginsenosides from North American ginseng using modified supercritical carbon dioxide
AU - Wood, Jeffery A.
AU - Bernards, Mark A.
AU - Wan, Wan kei
AU - Charpentier, Paul A.
PY - 2006/11/1
Y1 - 2006/11/1
N2 - The root of North American ginseng (Panax quinquefolius) was extracted using conventional solvent techniques (Soxhlet) and using supercritical fluid extraction (SFE). SFE was accomplished using modified supercritical carbon dioxide (scCO2), with methanol and DMSO as modifiers. At a temperature of 110 °C and a modifier to solid ratio of 4.1 g of modifier per gram of ginseng, the system pressure was found to have no significant effect on the quantity of ginsenosides extracted from 20.7 to 48.3 MPa using CO2 + methanol. In addition, the process was shown to be mass transfer/desorption limited rather than solubility limited under these conditions. For the studied SFE conditions, up to 90% of the total ginsenosides were extracted compared to conventional methanol Soxhlet, as determined by HPLC analysis. A relatively unknown ginsenoside peak was observed in the HPLC chromatograms for all supercritical extractions, particularly those employing DMSO as a modifier. This peak was identified as 6″/6″″-mono-O-acetyl ginsenoside Rb1 by LC/MS.
AB - The root of North American ginseng (Panax quinquefolius) was extracted using conventional solvent techniques (Soxhlet) and using supercritical fluid extraction (SFE). SFE was accomplished using modified supercritical carbon dioxide (scCO2), with methanol and DMSO as modifiers. At a temperature of 110 °C and a modifier to solid ratio of 4.1 g of modifier per gram of ginseng, the system pressure was found to have no significant effect on the quantity of ginsenosides extracted from 20.7 to 48.3 MPa using CO2 + methanol. In addition, the process was shown to be mass transfer/desorption limited rather than solubility limited under these conditions. For the studied SFE conditions, up to 90% of the total ginsenosides were extracted compared to conventional methanol Soxhlet, as determined by HPLC analysis. A relatively unknown ginsenoside peak was observed in the HPLC chromatograms for all supercritical extractions, particularly those employing DMSO as a modifier. This peak was identified as 6″/6″″-mono-O-acetyl ginsenoside Rb1 by LC/MS.
KW - Carbon dioxide
KW - Extraction
KW - Ginseng
KW - Ginsenosides
KW - Supercritical fluids
UR - http://www.scopus.com/inward/record.url?scp=33750181285&partnerID=8YFLogxK
U2 - 10.1016/j.supflu.2006.01.016
DO - 10.1016/j.supflu.2006.01.016
M3 - Article
VL - 39
SP - 40
EP - 47
JO - Journal of supercritical fluids
JF - Journal of supercritical fluids
SN - 0896-8446
IS - 1
ER -