Extraction of Lysozyme from Chicken Albumen Using Polyelectrolyte Complexes

Jéré J. van Lente, Saskia Lindhoud*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)
91 Downloads (Pure)

Abstract

Cells use droplet-like membrane-less organelles (MLOs) to compartmentalize and selectively take-up molecules, such as proteins, from their internal environment. These membraneless organelles can be mimicked by polyelectrolyte complexes (PECs) consisting of oppositely charged polyelectrolytes. Previous research has demonstrated that protein uptake strongly depends on the PEC composition. This suggests that PECs can be used to selectively extract proteins from a multi-protein mixture. With this in mind, the partitioning of the protein lysozyme in four PEC systems consisting of different weak and strong polyelectrolyte combinations is investigated. All systems show similar trends in lysozyme partitioning as a function of the complex composition. The release of lysozyme from complexes at their optimal lysozyme uptake composition is investigated by increasing the salt concentration to 500 mm NaCl or lowering the pH from 7 to 4. Complexes of poly(allylamine hydrochloride) and poly(acrylic acid) have the best uptake and release properties. These are used for selective extraction of lysozyme from a hen-egg white protein matrix. The (back)-extracted lysozyme retains its enzymatic activity, showing the capability of PECs to function as extraction media for proteins.

Original languageEnglish
Article number2105147
JournalSmall
Volume18
Issue number6
Early online date8 Dec 2021
DOIs
Publication statusPublished - 10 Feb 2022

Keywords

  • Egg whites
  • Extraction
  • Lysozyme
  • Membrane-less organelles
  • Polyelectrolyte complexes
  • Polyelectrolytes
  • Proteins
  • UT-Hybrid-D

Fingerprint

Dive into the research topics of 'Extraction of Lysozyme from Chicken Albumen Using Polyelectrolyte Complexes'. Together they form a unique fingerprint.

Cite this