Fabrication and characterization of hydroxyapatite-coated disks for use in osteoprogenitor cell culture

A. Jon Goldberg, Yongxing Liu, J. Liu, Maria C. Advincula, Gloria Gronowicz, Pamela Habibovic, Liisa T. Kuhn

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

A simple method is reported for fabricating polystyrene disk inserts coated with biomimetic carbonated hydroxyapatite (cHA) to be used for culturing osteoprogenitor cells or other stem cells. Roughened disks cut from tissue-culture polystyrene (TCPS) were coated in simulated body fluid with 5 × normal physiologic ionic concentrations (SBFx5) by a 2-step, 2-day method. The coatings were rigorously characterized by various methods and assessed in cell culture. An adherent, nearly 10 mm thick, relatively uniform layer of single-phase cHA was formed in two days. MC3T3-E1 and mouse calvaria-derived osteoprogenitor cells (pCOBs) were cultured on the cHA for various time points. Despite less initial attachment of both cell types to the cHA, proliferation rates on cHA were similar to that on TCPS. Two-fold greater cell attachment (P < 0.05) of the MC3T3-E1 cells was observed relative to the pCOBs, on both the TCPS and the cHA. Importantly, the coatings were relatively smooth, without the extensive agglomerates observed in other studies and remained adherent and morphologically unchanged after 21 days of culture. This technique can be used to rapidly produce high-quality cHA-coated TCPS disks for cell-culture studies
Original languageEnglish
Pages (from-to)1371-1378
JournalJournal of biomaterials science : polymer edition
Volume21
Issue number10
DOIs
Publication statusPublished - 2010

Keywords

  • IR-80849
  • METIS-273562

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