Fabrication of cell container arrays with overlaid surface topographies

R.K. Truckenmüller, S. Giselbrecht, M. Escalante Marun, M.N.W. Groenendijk, M.N.W. Groenendijk, B.J. Papenburg, N.C. Rivron, H.V. Unadkat, V. Saile, Vinod Subramaniam, Clemens van Blitterswijk, Albert van den Berg, Matthias Wessling, Jan de Boer, Dimitrios Stamatialis

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Abstract

This paper presents cell culture substrates in the form of microcontainer arrays with overlaid surface topographies, and a technology for their fabrication. The new fabrication technology is based on microscale thermoforming of thin polymer films whose surfaces are topographically prepatterned on a micro- or nanoscale. For microthermoforming, we apply a new process on the basis of temporary back moulding of polymer films and use the novel concept of a perforated-sheet-like mould. Thermal micro- or nanoimprinting is applied for prepatterning. The novel cell container arrays are fabricated from polylactic acid (PLA) films. The thin-walled microcontainer structures have the shape of a spherical calotte merging into a hexagonal shape at their upper circumferential edges. In the arrays, the cell containers are arranged densely packed in honeycomb fashion. The inner surfaces of the highly curved container walls are provided with various topographical micro- and nanopatterns. For a first validation of the microcontainer arrays as in vitro cell culture substrates, C2C12 mouse premyoblasts are cultured in containers with microgrooved surfaces and shown to align along the grooves in the three-dimensional film substrates. In future stem-cell-biological and tissue engineering applications, microcontainers fabricated using the proposed technology may act as geometrically defined artificial microenvironments or niches.
Original languageEnglish
Pages (from-to)95-107
Number of pages13
JournalBiomedical microdevices
Volume14
Issue number1
DOIs
Publication statusPublished - Feb 2012

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Surface topography
Containers
Technology
Fabrication
Polymers
Cell Culture Techniques
Cell culture
Polymer films
Cell Engineering
Bioengineering
Substrates
Motion Pictures
Tissue Engineering
Thin walled structures
Thermoforming
Fungi
Stem Cells
Hot Temperature
Stem cells
Tissue engineering

Keywords

  • IR-79976
  • EWI-21696
  • METIS-283267

Cite this

Truckenmüller, R. K., Giselbrecht, S., Escalante Marun, M., Groenendijk, M. N. W., Groenendijk, M. N. W., Papenburg, B. J., ... Stamatialis, D. (2012). Fabrication of cell container arrays with overlaid surface topographies. Biomedical microdevices, 14(1), 95-107. https://doi.org/10.1007/s10544-011-9588-5
Truckenmüller, R.K. ; Giselbrecht, S. ; Escalante Marun, M. ; Groenendijk, M.N.W. ; Groenendijk, M.N.W. ; Papenburg, B.J. ; Rivron, N.C. ; Unadkat, H.V. ; Saile, V. ; Subramaniam, Vinod ; van Blitterswijk, Clemens ; van den Berg, Albert ; Wessling, Matthias ; de Boer, Jan ; Stamatialis, Dimitrios. / Fabrication of cell container arrays with overlaid surface topographies. In: Biomedical microdevices. 2012 ; Vol. 14, No. 1. pp. 95-107.
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Truckenmüller, RK, Giselbrecht, S, Escalante Marun, M, Groenendijk, MNW, Groenendijk, MNW, Papenburg, BJ, Rivron, NC, Unadkat, HV, Saile, V, Subramaniam, V, van Blitterswijk, C, van den Berg, A, Wessling, M, de Boer, J & Stamatialis, D 2012, 'Fabrication of cell container arrays with overlaid surface topographies' Biomedical microdevices, vol. 14, no. 1, pp. 95-107. https://doi.org/10.1007/s10544-011-9588-5

Fabrication of cell container arrays with overlaid surface topographies. / Truckenmüller, R.K.; Giselbrecht, S.; Escalante Marun, M.; Groenendijk, M.N.W.; Groenendijk, M.N.W.; Papenburg, B.J.; Rivron, N.C.; Unadkat, H.V.; Saile, V.; Subramaniam, Vinod; van Blitterswijk, Clemens; van den Berg, Albert; Wessling, Matthias; de Boer, Jan; Stamatialis, Dimitrios.

In: Biomedical microdevices, Vol. 14, No. 1, 02.2012, p. 95-107.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Truckenmüller, R.K.

AU - Giselbrecht, S.

AU - Escalante Marun, M.

AU - Groenendijk, M.N.W.

AU - Groenendijk, M.N.W.

AU - Papenburg, B.J.

AU - Rivron, N.C.

AU - Unadkat, H.V.

AU - Saile, V.

AU - Subramaniam, Vinod

AU - van Blitterswijk, Clemens

AU - van den Berg, Albert

AU - Wessling, Matthias

AU - de Boer, Jan

AU - Stamatialis, Dimitrios

N1 - Open Access

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N2 - This paper presents cell culture substrates in the form of microcontainer arrays with overlaid surface topographies, and a technology for their fabrication. The new fabrication technology is based on microscale thermoforming of thin polymer films whose surfaces are topographically prepatterned on a micro- or nanoscale. For microthermoforming, we apply a new process on the basis of temporary back moulding of polymer films and use the novel concept of a perforated-sheet-like mould. Thermal micro- or nanoimprinting is applied for prepatterning. The novel cell container arrays are fabricated from polylactic acid (PLA) films. The thin-walled microcontainer structures have the shape of a spherical calotte merging into a hexagonal shape at their upper circumferential edges. In the arrays, the cell containers are arranged densely packed in honeycomb fashion. The inner surfaces of the highly curved container walls are provided with various topographical micro- and nanopatterns. For a first validation of the microcontainer arrays as in vitro cell culture substrates, C2C12 mouse premyoblasts are cultured in containers with microgrooved surfaces and shown to align along the grooves in the three-dimensional film substrates. In future stem-cell-biological and tissue engineering applications, microcontainers fabricated using the proposed technology may act as geometrically defined artificial microenvironments or niches.

AB - This paper presents cell culture substrates in the form of microcontainer arrays with overlaid surface topographies, and a technology for their fabrication. The new fabrication technology is based on microscale thermoforming of thin polymer films whose surfaces are topographically prepatterned on a micro- or nanoscale. For microthermoforming, we apply a new process on the basis of temporary back moulding of polymer films and use the novel concept of a perforated-sheet-like mould. Thermal micro- or nanoimprinting is applied for prepatterning. The novel cell container arrays are fabricated from polylactic acid (PLA) films. The thin-walled microcontainer structures have the shape of a spherical calotte merging into a hexagonal shape at their upper circumferential edges. In the arrays, the cell containers are arranged densely packed in honeycomb fashion. The inner surfaces of the highly curved container walls are provided with various topographical micro- and nanopatterns. For a first validation of the microcontainer arrays as in vitro cell culture substrates, C2C12 mouse premyoblasts are cultured in containers with microgrooved surfaces and shown to align along the grooves in the three-dimensional film substrates. In future stem-cell-biological and tissue engineering applications, microcontainers fabricated using the proposed technology may act as geometrically defined artificial microenvironments or niches.

KW - IR-79976

KW - EWI-21696

KW - METIS-283267

U2 - 10.1007/s10544-011-9588-5

DO - 10.1007/s10544-011-9588-5

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SP - 95

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JF - Biomedical microdevices

SN - 1387-2176

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Truckenmüller RK, Giselbrecht S, Escalante Marun M, Groenendijk MNW, Groenendijk MNW, Papenburg BJ et al. Fabrication of cell container arrays with overlaid surface topographies. Biomedical microdevices. 2012 Feb;14(1):95-107. https://doi.org/10.1007/s10544-011-9588-5