Fast in-situ formation of dextran-tyramine hydrogels for in vitro chondrocyte culturing

R. Jin, W.E. Hennink (Editor), J. Feijen (Editor), Liliana Moreira Teixeira, Pieter J. Dijkstra, A.P. Sam (Editor), Hermanus Bernardus Johannes Karperien, Zhiyuan Zhong, Jan Feijen

Research output: Contribution to conferencePaperAcademic

Abstract

Dextran hydrogels were formed in situ by enzymatic crosslinking of dextran-tyramine conjugates (dex-TA)s using horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). Depending on the molecular weight (Mn) of dextran and the degree of substitution (DS) of dextran with tyramine groups, the gelation time varied from about 20 s to a few minutes. Hydrogels prepared from dex-TA (Mn = 3.1 × 104 g/mol) with a DS of 10 had storage moduli up to 60 kPa. Moreover, hydrogels in which chondrocytes were encapsulated had moduli comparable to those without cells. The H2O2/TA mole ratio applied for the preparation of the hydrogels influenced the cell viability of chondrocytes cultured in the hydrogels. A live-dead assay revealed that almost all chondrocytes retained their viability when cultured in hydrogels prepared with a relatively low H2O2/TA molar ratio of 0.2. Histological analysis showed that the encapsulated chondrocytes are capable of maintaining their phenotype as confirmed by the round shape of the cells and production of proteoglycans up to 3 weeks.
Original languageUndefined
Pagese24-e26
DOIs
Publication statusPublished - 2 Apr 2008
Event10th European Symposium on Controlled Drug Delivery, ESCDD 2008 - Noordwijk aan Zee, Netherlands
Duration: 2 Apr 20084 Apr 2008
Conference number: 10

Conference

Conference10th European Symposium on Controlled Drug Delivery, ESCDD 2008
Abbreviated titleESCDD
CountryNetherlands
CityNoordwijk aan Zee
Period2/04/084/04/08

Keywords

  • IR-69279
  • METIS-254491

Cite this

Jin, R., Hennink, W. E. (Ed.), Feijen, J. (Ed.), Moreira Teixeira, L., Dijkstra, P. J., Sam, A. P. (Ed.), ... Feijen, J. (2008). Fast in-situ formation of dextran-tyramine hydrogels for in vitro chondrocyte culturing. e24-e26. Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands. https://doi.org/10.1016/j.jconrel.2008.09.014
Jin, R. ; Hennink, W.E. (Editor) ; Feijen, J. (Editor) ; Moreira Teixeira, Liliana ; Dijkstra, Pieter J. ; Sam, A.P. (Editor) ; Karperien, Hermanus Bernardus Johannes ; Zhong, Zhiyuan ; Feijen, Jan. / Fast in-situ formation of dextran-tyramine hydrogels for in vitro chondrocyte culturing. Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands.
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title = "Fast in-situ formation of dextran-tyramine hydrogels for in vitro chondrocyte culturing",
abstract = "Dextran hydrogels were formed in situ by enzymatic crosslinking of dextran-tyramine conjugates (dex-TA)s using horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). Depending on the molecular weight (Mn) of dextran and the degree of substitution (DS) of dextran with tyramine groups, the gelation time varied from about 20 s to a few minutes. Hydrogels prepared from dex-TA (Mn = 3.1 × 104 g/mol) with a DS of 10 had storage moduli up to 60 kPa. Moreover, hydrogels in which chondrocytes were encapsulated had moduli comparable to those without cells. The H2O2/TA mole ratio applied for the preparation of the hydrogels influenced the cell viability of chondrocytes cultured in the hydrogels. A live-dead assay revealed that almost all chondrocytes retained their viability when cultured in hydrogels prepared with a relatively low H2O2/TA molar ratio of 0.2. Histological analysis showed that the encapsulated chondrocytes are capable of maintaining their phenotype as confirmed by the round shape of the cells and production of proteoglycans up to 3 weeks.",
keywords = "IR-69279, METIS-254491",
author = "R. Jin and W.E. Hennink and J. Feijen and {Moreira Teixeira}, Liliana and Dijkstra, {Pieter J.} and A.P. Sam and Karperien, {Hermanus Bernardus Johannes} and Zhiyuan Zhong and Jan Feijen",
year = "2008",
month = "4",
day = "2",
doi = "10.1016/j.jconrel.2008.09.014",
language = "Undefined",
pages = "e24--e26",
note = "null ; Conference date: 02-04-2008 Through 04-04-2008",

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Jin, R, Hennink, WE (ed.), Feijen, J (ed.), Moreira Teixeira, L, Dijkstra, PJ, Sam, AP (ed.), Karperien, HBJ, Zhong, Z & Feijen, J 2008, 'Fast in-situ formation of dextran-tyramine hydrogels for in vitro chondrocyte culturing' Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands, 2/04/08 - 4/04/08, pp. e24-e26. https://doi.org/10.1016/j.jconrel.2008.09.014

Fast in-situ formation of dextran-tyramine hydrogels for in vitro chondrocyte culturing. / Jin, R.; Hennink, W.E. (Editor); Feijen, J. (Editor); Moreira Teixeira, Liliana; Dijkstra, Pieter J.; Sam, A.P. (Editor); Karperien, Hermanus Bernardus Johannes; Zhong, Zhiyuan; Feijen, Jan.

2008. e24-e26 Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands.

Research output: Contribution to conferencePaperAcademic

TY - CONF

T1 - Fast in-situ formation of dextran-tyramine hydrogels for in vitro chondrocyte culturing

AU - Jin, R.

AU - Moreira Teixeira, Liliana

AU - Dijkstra, Pieter J.

AU - Karperien, Hermanus Bernardus Johannes

AU - Zhong, Zhiyuan

AU - Feijen, Jan

A2 - Hennink, W.E.

A2 - Feijen, J.

A2 - Sam, A.P.

PY - 2008/4/2

Y1 - 2008/4/2

N2 - Dextran hydrogels were formed in situ by enzymatic crosslinking of dextran-tyramine conjugates (dex-TA)s using horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). Depending on the molecular weight (Mn) of dextran and the degree of substitution (DS) of dextran with tyramine groups, the gelation time varied from about 20 s to a few minutes. Hydrogels prepared from dex-TA (Mn = 3.1 × 104 g/mol) with a DS of 10 had storage moduli up to 60 kPa. Moreover, hydrogels in which chondrocytes were encapsulated had moduli comparable to those without cells. The H2O2/TA mole ratio applied for the preparation of the hydrogels influenced the cell viability of chondrocytes cultured in the hydrogels. A live-dead assay revealed that almost all chondrocytes retained their viability when cultured in hydrogels prepared with a relatively low H2O2/TA molar ratio of 0.2. Histological analysis showed that the encapsulated chondrocytes are capable of maintaining their phenotype as confirmed by the round shape of the cells and production of proteoglycans up to 3 weeks.

AB - Dextran hydrogels were formed in situ by enzymatic crosslinking of dextran-tyramine conjugates (dex-TA)s using horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). Depending on the molecular weight (Mn) of dextran and the degree of substitution (DS) of dextran with tyramine groups, the gelation time varied from about 20 s to a few minutes. Hydrogels prepared from dex-TA (Mn = 3.1 × 104 g/mol) with a DS of 10 had storage moduli up to 60 kPa. Moreover, hydrogels in which chondrocytes were encapsulated had moduli comparable to those without cells. The H2O2/TA mole ratio applied for the preparation of the hydrogels influenced the cell viability of chondrocytes cultured in the hydrogels. A live-dead assay revealed that almost all chondrocytes retained their viability when cultured in hydrogels prepared with a relatively low H2O2/TA molar ratio of 0.2. Histological analysis showed that the encapsulated chondrocytes are capable of maintaining their phenotype as confirmed by the round shape of the cells and production of proteoglycans up to 3 weeks.

KW - IR-69279

KW - METIS-254491

U2 - 10.1016/j.jconrel.2008.09.014

DO - 10.1016/j.jconrel.2008.09.014

M3 - Paper

SP - e24-e26

ER -

Jin R, Hennink WE, (ed.), Feijen J, (ed.), Moreira Teixeira L, Dijkstra PJ, Sam AP, (ed.) et al. Fast in-situ formation of dextran-tyramine hydrogels for in vitro chondrocyte culturing. 2008. Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands. https://doi.org/10.1016/j.jconrel.2008.09.014