Fast, ultrasensitive virus detection using a Young interferometer sensor

Aurel Ymeti, Jan Greve, Paul Lambeck, Thijs Wink, Stephan W.F.M. van Hovell, Tom A.M. Beumer, Robert Raimond Wijn, Rene Heideman, Vinod Subramaniam, Johannes S. Kanger

Research output: Contribution to journalArticleAcademicpeer-review

210 Citations (Scopus)

Abstract

We report the application of an integrated optical Young interferometer sensor for ultrasensitive, real-time, direct detection of viruses. We have validated the sensor by detecting herpes simplex virus type 1 (HSV-1), but the principle is generally applicable. Detection of HSV-1 virus particles was performed by applying the virus sample onto a sensor surface coated with a specific antibody against HSV-1. The performance of the sensor was tested by monitoring virus samples at clinically relevant concentrations. We show that the Young interferometer sensor can specifically and sensitively detect HSV-1 at very low concentrations (850 particles/mL). We have further demonstrated that the sensor can specifically detect HSV-1 suspended in serum. Extrapolation of the results indicates that the sensitivity of the sensor approaches the detection of a single virus particle binding, yielding a sensor of unprecedented sensitivity with wide applications for viral diagnostics.
Original languageEnglish
Pages (from-to)394-397
Number of pages4
JournalNano letters
Volume7
Issue number500-266/2
DOIs
Publication statusPublished - 2007

Keywords

  • METIS-237829
  • EWI-8848
  • IOMS-SNS: SENSORS
  • IR-59471

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    Ymeti, A., Greve, J., Lambeck, P., Wink, T., van Hovell, S. W. F. M., Beumer, T. A. M., ... Kanger, J. S. (2007). Fast, ultrasensitive virus detection using a Young interferometer sensor. Nano letters, 7(500-266/2), 394-397. https://doi.org/10.1021/nl062595n