We report the application of an integrated optical Young interferometer sensor for ultrasensitive, real-time, direct detection of viruses. We have validated the sensor by detecting herpes simplex virus type 1 (HSV-1), but the principle is generally applicable. Detection of HSV-1 virus particles was performed by applying the virus sample onto a sensor surface coated with a specific antibody against HSV-1. The performance of the sensor was tested by monitoring virus samples at clinically relevant concentrations. We show that the Young interferometer sensor can specifically and sensitively detect HSV-1 at very low concentrations (850 particles/mL). We have further demonstrated that the sensor can specifically detect HSV-1 suspended in serum. Extrapolation of the results indicates that the sensitivity of the sensor approaches the detection of a single virus particle binding, yielding a sensor of unprecedented sensitivity with wide applications for viral diagnostics.
- IOMS-SNS: SENSORS
Ymeti, A., Greve, J., Lambeck, P., Wink, T., van Hovell, S. W. F. M., Beumer, T. A. M., ... Kanger, J. S. (2007). Fast, ultrasensitive virus detection using a Young interferometer sensor. Nano letters, 7(500-266/2), 394-397. https://doi.org/10.1021/nl062595n