TY - JOUR
T1 - Feasibility of evanescent wave interferometer immunosensors for pesticide detection
T2 - chemical aspects
AU - Lechuga, L.M.
AU - Lenferink, A.T.M.
AU - Kooyman, R.P.H.
AU - Greve, J.
PY - 1995
Y1 - 1995
N2 - A waveguide Mach-Zehnder Interferometer (MZI) immunosensor has been developed which can detect, in a direct way, a minimum average layer growth thickness of the analyte of 2×10−3 nm (bound mass, I′=1×10−4 δ cm−2). The design and fabrication of the sensor and the experimental set-up are aimed at decreasing the detection limit. A flow cell has been designed and incorporated into the set-up to allow a high mass-transport rate of the analyte molecules to the sensor surface, in this way decreasing the net response time. Results for the immunosystem α-hSA/hSA are presented. All improvements have been done as a first step towards the use of the MZI immunosensor in the direct detection of low coverages of pesticides with an expected layer growth of 2×10−4 nm.
AB - A waveguide Mach-Zehnder Interferometer (MZI) immunosensor has been developed which can detect, in a direct way, a minimum average layer growth thickness of the analyte of 2×10−3 nm (bound mass, I′=1×10−4 δ cm−2). The design and fabrication of the sensor and the experimental set-up are aimed at decreasing the detection limit. A flow cell has been designed and incorporated into the set-up to allow a high mass-transport rate of the analyte molecules to the sensor surface, in this way decreasing the net response time. Results for the immunosystem α-hSA/hSA are presented. All improvements have been done as a first step towards the use of the MZI immunosensor in the direct detection of low coverages of pesticides with an expected layer growth of 2×10−4 nm.
U2 - 10.1016/0925-4005(95)85169-0
DO - 10.1016/0925-4005(95)85169-0
M3 - Article
VL - 25
SP - 762
EP - 765
JO - Sensors and actuators. B: Chemical
JF - Sensors and actuators. B: Chemical
SN - 0925-4005
IS - 24-25
ER -