Ferro-based derivatizing agents for LC/MS an LC/EC/MS

Bettina Seiwert

    Research output: ThesisPhD Thesis - Research UT, graduation UTAcademic

    26 Downloads (Pure)

    Abstract

    Within this thesis, the development and application of ferrocene-based derivatizing agents for LC/MS and LC/EC/MS is presented. The advantages of derivatization by ferrocenes are the similtaneous introduction of a mass tag and an electroactive group, which make them ideally suited for LC/MS and especially LC/EC/MS detection. Furthermore, due to the reaction of polar functionalities with less polar labels, the HPLC reversed-phase separation is strongly improved. Thiols and isocyanates have to be derivatized because of their instability. Two new ferrocene-based derivatizing agents were developed for these analytes: Ferroceneoyl piperazide (Fc-Pz) for isocyanates and ferrocenecarboxylic acid-(2- maleimidoyl)ethylamide (FMEA) for thiols. Both derivatizing agents are highly reactive towards the respective functional groups and yield stable derivatives.
    Original languageEnglish
    Awarding Institution
    • University of Twente
    Supervisors/Advisors
    • Karst, Uwe, Supervisor
    Award date6 Jul 2018
    Publisher
    Print ISBNs978-90-365-2532-9
    Publication statusPublished - 6 Jul 2007

    Fingerprint

    Isocyanates
    Sulfhydryl Compounds
    Phase separation
    Functional groups
    Labels
    Derivatives
    ferrocene
    ferrocenecarboxylic acid

    Keywords

    • IR-57888

    Cite this

    Seiwert, B. (2007). Ferro-based derivatizing agents for LC/MS an LC/EC/MS. University of Twente.
    Seiwert, Bettina. / Ferro-based derivatizing agents for LC/MS an LC/EC/MS. University of Twente, 2007. 182 p.
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    title = "Ferro-based derivatizing agents for LC/MS an LC/EC/MS",
    abstract = "Within this thesis, the development and application of ferrocene-based derivatizing agents for LC/MS and LC/EC/MS is presented. The advantages of derivatization by ferrocenes are the similtaneous introduction of a mass tag and an electroactive group, which make them ideally suited for LC/MS and especially LC/EC/MS detection. Furthermore, due to the reaction of polar functionalities with less polar labels, the HPLC reversed-phase separation is strongly improved. Thiols and isocyanates have to be derivatized because of their instability. Two new ferrocene-based derivatizing agents were developed for these analytes: Ferroceneoyl piperazide (Fc-Pz) for isocyanates and ferrocenecarboxylic acid-(2- maleimidoyl)ethylamide (FMEA) for thiols. Both derivatizing agents are highly reactive towards the respective functional groups and yield stable derivatives.",
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    author = "Bettina Seiwert",
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    Seiwert, B 2007, 'Ferro-based derivatizing agents for LC/MS an LC/EC/MS', University of Twente.

    Ferro-based derivatizing agents for LC/MS an LC/EC/MS. / Seiwert, Bettina.

    University of Twente, 2007. 182 p.

    Research output: ThesisPhD Thesis - Research UT, graduation UTAcademic

    TY - THES

    T1 - Ferro-based derivatizing agents for LC/MS an LC/EC/MS

    AU - Seiwert, Bettina

    PY - 2007/7/6

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    N2 - Within this thesis, the development and application of ferrocene-based derivatizing agents for LC/MS and LC/EC/MS is presented. The advantages of derivatization by ferrocenes are the similtaneous introduction of a mass tag and an electroactive group, which make them ideally suited for LC/MS and especially LC/EC/MS detection. Furthermore, due to the reaction of polar functionalities with less polar labels, the HPLC reversed-phase separation is strongly improved. Thiols and isocyanates have to be derivatized because of their instability. Two new ferrocene-based derivatizing agents were developed for these analytes: Ferroceneoyl piperazide (Fc-Pz) for isocyanates and ferrocenecarboxylic acid-(2- maleimidoyl)ethylamide (FMEA) for thiols. Both derivatizing agents are highly reactive towards the respective functional groups and yield stable derivatives.

    AB - Within this thesis, the development and application of ferrocene-based derivatizing agents for LC/MS and LC/EC/MS is presented. The advantages of derivatization by ferrocenes are the similtaneous introduction of a mass tag and an electroactive group, which make them ideally suited for LC/MS and especially LC/EC/MS detection. Furthermore, due to the reaction of polar functionalities with less polar labels, the HPLC reversed-phase separation is strongly improved. Thiols and isocyanates have to be derivatized because of their instability. Two new ferrocene-based derivatizing agents were developed for these analytes: Ferroceneoyl piperazide (Fc-Pz) for isocyanates and ferrocenecarboxylic acid-(2- maleimidoyl)ethylamide (FMEA) for thiols. Both derivatizing agents are highly reactive towards the respective functional groups and yield stable derivatives.

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    M3 - PhD Thesis - Research UT, graduation UT

    SN - 978-90-365-2532-9

    PB - University of Twente

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    Seiwert B. Ferro-based derivatizing agents for LC/MS an LC/EC/MS. University of Twente, 2007. 182 p.