Ferro-based derivatizing agents for LC/MS an LC/EC/MS

Bettina Seiwert

Ferro-based derivatizing agents for LC/MS an LC/EC/MS

Bettina Seiwert

Research output: Thesis › PhD Thesis - Research UT, graduation UT

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Abstract

Within this thesis, the development and application of ferrocene-based derivatizing agents for LC/MS and LC/EC/MS is presented. The advantages of derivatization by ferrocenes are the similtaneous introduction of a mass tag and an electroactive group, which make them ideally suited for LC/MS and especially LC/EC/MS detection. Furthermore, due to the reaction of polar functionalities with less polar labels, the HPLC reversed-phase separation is strongly improved. Thiols and isocyanates have to be derivatized because of their instability. Two new ferrocene-based derivatizing agents were developed for these analytes: Ferroceneoyl piperazide (Fc-Pz) for isocyanates and ferrocenecarboxylic acid-(2- maleimidoyl)ethylamide (FMEA) for thiols. Both derivatizing agents are highly reactive towards the respective functional groups and yield stable derivatives.

Original language	English
Awarding Institution	University of Twente
Supervisors/Advisors	Karst, Uwe, Supervisor
Award date	6 Jul 2018
Publisher	University of Twente
Print ISBNs	978-90-365-2532-9
Publication status	Published - 6 Jul 2007

Keywords

IR-57888

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title = "Ferro-based derivatizing agents for LC/MS an LC/EC/MS",

abstract = "Within this thesis, the development and application of ferrocene-based derivatizing agents for LC/MS and LC/EC/MS is presented. The advantages of derivatization by ferrocenes are the similtaneous introduction of a mass tag and an electroactive group, which make them ideally suited for LC/MS and especially LC/EC/MS detection. Furthermore, due to the reaction of polar functionalities with less polar labels, the HPLC reversed-phase separation is strongly improved. Thiols and isocyanates have to be derivatized because of their instability. Two new ferrocene-based derivatizing agents were developed for these analytes: Ferroceneoyl piperazide (Fc-Pz) for isocyanates and ferrocenecarboxylic acid-(2- maleimidoyl)ethylamide (FMEA) for thiols. Both derivatizing agents are highly reactive towards the respective functional groups and yield stable derivatives.",

keywords = "IR-57888",

author = "Bettina Seiwert",

year = "2007",

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day = "6",

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TY - THES

T1 - Ferro-based derivatizing agents for LC/MS an LC/EC/MS

AU - Seiwert, Bettina

PY - 2007/7/6

Y1 - 2007/7/6

N2 - Within this thesis, the development and application of ferrocene-based derivatizing agents for LC/MS and LC/EC/MS is presented. The advantages of derivatization by ferrocenes are the similtaneous introduction of a mass tag and an electroactive group, which make them ideally suited for LC/MS and especially LC/EC/MS detection. Furthermore, due to the reaction of polar functionalities with less polar labels, the HPLC reversed-phase separation is strongly improved. Thiols and isocyanates have to be derivatized because of their instability. Two new ferrocene-based derivatizing agents were developed for these analytes: Ferroceneoyl piperazide (Fc-Pz) for isocyanates and ferrocenecarboxylic acid-(2- maleimidoyl)ethylamide (FMEA) for thiols. Both derivatizing agents are highly reactive towards the respective functional groups and yield stable derivatives.

AB - Within this thesis, the development and application of ferrocene-based derivatizing agents for LC/MS and LC/EC/MS is presented. The advantages of derivatization by ferrocenes are the similtaneous introduction of a mass tag and an electroactive group, which make them ideally suited for LC/MS and especially LC/EC/MS detection. Furthermore, due to the reaction of polar functionalities with less polar labels, the HPLC reversed-phase separation is strongly improved. Thiols and isocyanates have to be derivatized because of their instability. Two new ferrocene-based derivatizing agents were developed for these analytes: Ferroceneoyl piperazide (Fc-Pz) for isocyanates and ferrocenecarboxylic acid-(2- maleimidoyl)ethylamide (FMEA) for thiols. Both derivatizing agents are highly reactive towards the respective functional groups and yield stable derivatives.

KW - IR-57888

M3 - PhD Thesis - Research UT, graduation UT

SN - 978-90-365-2532-9

PB - University of Twente

ER -

Ferro-based derivatizing agents for LC/MS an LC/EC/MS

Abstract

Keywords

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