Ferro-based derivatizing agents for LC/MS an LC/EC/MS

Bettina Seiwert

    Research output: ThesisPhD Thesis - Research UT, graduation UT

    66 Downloads (Pure)


    Within this thesis, the development and application of ferrocene-based derivatizing agents for LC/MS and LC/EC/MS is presented. The advantages of derivatization by ferrocenes are the similtaneous introduction of a mass tag and an electroactive group, which make them ideally suited for LC/MS and especially LC/EC/MS detection. Furthermore, due to the reaction of polar functionalities with less polar labels, the HPLC reversed-phase separation is strongly improved. Thiols and isocyanates have to be derivatized because of their instability. Two new ferrocene-based derivatizing agents were developed for these analytes: Ferroceneoyl piperazide (Fc-Pz) for isocyanates and ferrocenecarboxylic acid-(2- maleimidoyl)ethylamide (FMEA) for thiols. Both derivatizing agents are highly reactive towards the respective functional groups and yield stable derivatives.
    Original languageEnglish
    Awarding Institution
    • University of Twente
    • Karst, Uwe, Supervisor
    Award date6 Jul 2018
    Print ISBNs978-90-365-2532-9
    Publication statusPublished - 6 Jul 2007


    • IR-57888


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