Within this thesis, the development and application of ferrocene-based derivatizing agents for LC/MS and LC/EC/MS is presented. The advantages of derivatization by ferrocenes are the similtaneous introduction of a mass tag and an electroactive group, which make them ideally suited for LC/MS and especially LC/EC/MS detection. Furthermore, due to the reaction of polar functionalities with less polar labels, the HPLC reversed-phase separation is strongly improved. Thiols and isocyanates have to be derivatized because of their instability. Two new ferrocene-based derivatizing agents were developed for these analytes: Ferroceneoyl piperazide (Fc-Pz) for isocyanates and ferrocenecarboxylic acid-(2- maleimidoyl)ethylamide (FMEA) for thiols. Both derivatizing agents are highly reactive towards the respective functional groups and yield stable derivatives.
|Award date||6 Jul 2018|
|Publication status||Published - 6 Jul 2007|