Abstract
Within this thesis, the development and application of ferrocene-based derivatizing agents for LC/MS and LC/EC/MS is presented.
The advantages of derivatization by ferrocenes are the similtaneous introduction of a mass tag and an electroactive group, which make them ideally suited for LC/MS and especially LC/EC/MS detection. Furthermore,
due to the reaction of polar functionalities with less polar labels, the HPLC reversed-phase separation is strongly improved. Thiols and isocyanates have
to be derivatized because of their instability. Two new ferrocene-based derivatizing agents were developed for these analytes: Ferroceneoyl piperazide (Fc-Pz) for isocyanates and ferrocenecarboxylic acid-(2-
maleimidoyl)ethylamide (FMEA) for thiols. Both derivatizing agents are highly reactive towards the respective functional groups and yield stable derivatives.
| Original language | English |
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| Qualification | Doctor of Philosophy |
| Awarding Institution |
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| Supervisors/Advisors |
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| Award date | 6 Jul 2007 |
| Place of Publication | Enschede |
| Publisher | |
| Print ISBNs | 978-90-365-2532-9 |
| DOIs | |
| Publication status | Published - 6 Jul 2007 |
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