Field-dependent DNA mobility in 20nm high nanoslits

G.B. Beugelaar, Juliane Teapal, Jan William van Nieuwkasteele, Daniël Wijnperle, Daniel Wijnperle, Jonas O. Tegenfeldt, Fred Lisdat, Albert van den Berg, Jan C.T. Eijkel

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

The transport behavior of lambda-DNA (48 kbp) in fused silica nanoslits is investigated upon application of electrical fields of different strengths. The slit dimensions are 20 nm in height, 3 mu m in width, and 500 mu m in length. With fields of 30 kV/m or below, the molecules move fluently through the slits, while at higher electrical fields, the DNA molecules move intermittently, resulting in a strongly reduced mobility. We propose that the behavior can be explained by mechanical and/or field-induced dielectrophoretic DNA trapping due to the surface roughness in the nanoslits. The observation of preferential pathways and trapping sites of the lambda-DNA molecules through the nanoslits supports this hypothesis and indicates that the classical viscous friction models to explain the DNA movement in nanoslits needs to be modified to include these effects. Preliminary experiments with the smaller Xbal-digested litmus-DNA (2.8 kbp) show that the behavior is size-dependent, suggesting that the high field electrophoresis in nanoslits can be used for DNA separation.
Original languageUndefined
Pages (from-to)1785-1790
Number of pages6
JournalNano letters
Volume8
Issue number08332/7
DOIs
Publication statusPublished - Jul 2008

Keywords

  • METIS-252109
  • EWI-14082
  • BIOS-Micro/nanofluidics
  • IR-59461

Cite this

Beugelaar, G.B. ; Teapal, Juliane ; van Nieuwkasteele, Jan William ; Wijnperle, Daniël ; Wijnperle, Daniel ; Tegenfeldt, Jonas O. ; Lisdat, Fred ; van den Berg, Albert ; Eijkel, Jan C.T. / Field-dependent DNA mobility in 20nm high nanoslits. In: Nano letters. 2008 ; Vol. 8, No. 08332/7. pp. 1785-1790.
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title = "Field-dependent DNA mobility in 20nm high nanoslits",
abstract = "The transport behavior of lambda-DNA (48 kbp) in fused silica nanoslits is investigated upon application of electrical fields of different strengths. The slit dimensions are 20 nm in height, 3 mu m in width, and 500 mu m in length. With fields of 30 kV/m or below, the molecules move fluently through the slits, while at higher electrical fields, the DNA molecules move intermittently, resulting in a strongly reduced mobility. We propose that the behavior can be explained by mechanical and/or field-induced dielectrophoretic DNA trapping due to the surface roughness in the nanoslits. The observation of preferential pathways and trapping sites of the lambda-DNA molecules through the nanoslits supports this hypothesis and indicates that the classical viscous friction models to explain the DNA movement in nanoslits needs to be modified to include these effects. Preliminary experiments with the smaller Xbal-digested litmus-DNA (2.8 kbp) show that the behavior is size-dependent, suggesting that the high field electrophoresis in nanoslits can be used for DNA separation.",
keywords = "METIS-252109, EWI-14082, BIOS-Micro/nanofluidics, IR-59461",
author = "G.B. Beugelaar and Juliane Teapal and {van Nieuwkasteele}, {Jan William} and Dani{\"e}l Wijnperle and Daniel Wijnperle and Tegenfeldt, {Jonas O.} and Fred Lisdat and {van den Berg}, Albert and Eijkel, {Jan C.T.}",
note = "10.1021/nl080300v",
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Beugelaar, GB, Teapal, J, van Nieuwkasteele, JW, Wijnperle, D, Wijnperle, D, Tegenfeldt, JO, Lisdat, F, van den Berg, A & Eijkel, JCT 2008, 'Field-dependent DNA mobility in 20nm high nanoslits' Nano letters, vol. 8, no. 08332/7, pp. 1785-1790. https://doi.org/10.1021/nl080300v

Field-dependent DNA mobility in 20nm high nanoslits. / Beugelaar, G.B.; Teapal, Juliane; van Nieuwkasteele, Jan William; Wijnperle, Daniël; Wijnperle, Daniel; Tegenfeldt, Jonas O.; Lisdat, Fred; van den Berg, Albert; Eijkel, Jan C.T.

In: Nano letters, Vol. 8, No. 08332/7, 07.2008, p. 1785-1790.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Field-dependent DNA mobility in 20nm high nanoslits

AU - Beugelaar, G.B.

AU - Teapal, Juliane

AU - van Nieuwkasteele, Jan William

AU - Wijnperle, Daniël

AU - Wijnperle, Daniel

AU - Tegenfeldt, Jonas O.

AU - Lisdat, Fred

AU - van den Berg, Albert

AU - Eijkel, Jan C.T.

N1 - 10.1021/nl080300v

PY - 2008/7

Y1 - 2008/7

N2 - The transport behavior of lambda-DNA (48 kbp) in fused silica nanoslits is investigated upon application of electrical fields of different strengths. The slit dimensions are 20 nm in height, 3 mu m in width, and 500 mu m in length. With fields of 30 kV/m or below, the molecules move fluently through the slits, while at higher electrical fields, the DNA molecules move intermittently, resulting in a strongly reduced mobility. We propose that the behavior can be explained by mechanical and/or field-induced dielectrophoretic DNA trapping due to the surface roughness in the nanoslits. The observation of preferential pathways and trapping sites of the lambda-DNA molecules through the nanoslits supports this hypothesis and indicates that the classical viscous friction models to explain the DNA movement in nanoslits needs to be modified to include these effects. Preliminary experiments with the smaller Xbal-digested litmus-DNA (2.8 kbp) show that the behavior is size-dependent, suggesting that the high field electrophoresis in nanoslits can be used for DNA separation.

AB - The transport behavior of lambda-DNA (48 kbp) in fused silica nanoslits is investigated upon application of electrical fields of different strengths. The slit dimensions are 20 nm in height, 3 mu m in width, and 500 mu m in length. With fields of 30 kV/m or below, the molecules move fluently through the slits, while at higher electrical fields, the DNA molecules move intermittently, resulting in a strongly reduced mobility. We propose that the behavior can be explained by mechanical and/or field-induced dielectrophoretic DNA trapping due to the surface roughness in the nanoslits. The observation of preferential pathways and trapping sites of the lambda-DNA molecules through the nanoslits supports this hypothesis and indicates that the classical viscous friction models to explain the DNA movement in nanoslits needs to be modified to include these effects. Preliminary experiments with the smaller Xbal-digested litmus-DNA (2.8 kbp) show that the behavior is size-dependent, suggesting that the high field electrophoresis in nanoslits can be used for DNA separation.

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KW - IR-59461

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JO - Nano letters

JF - Nano letters

SN - 1530-6984

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ER -

Beugelaar GB, Teapal J, van Nieuwkasteele JW, Wijnperle D, Wijnperle D, Tegenfeldt JO et al. Field-dependent DNA mobility in 20nm high nanoslits. Nano letters. 2008 Jul;8(08332/7):1785-1790. https://doi.org/10.1021/nl080300v