Flow cytometric determination of circulating immune complexes with the indirect granulocyte phagocytosis test

L.W.M.M. Terstappen; B.G. de Grooth; G.M.J. Nolten; C.H.H. ten Napel; W. van Berkel; J. Greve

doi:10.1002/cyto.990060407

Flow cytometric determination of circulating immune complexes with the indirect granulocyte phagocytosis test

L.W.M.M. Terstappen
, B.G. de Grooth
, G.M.J. Nolten
, C.H.H. ten Napel
, W. van Berkel
, J. Greve

Research output: Contribution to journal › Article › Academic

6 Citations (Scopus)

212 Downloads (Pure)

Abstract

A method for the determination of circulating immune complexes (CIC) was adapted for flow cytometric analysis. Human granulocytes were used to phagocytose IgG-bearing CIC of serum from systemic lupus erythematosus (SLE) patients. A method for labeling the phagocytosed CIC with FITC-conjugated anti-human IgG was developed where the granulocytes remain in suspension during fixation and labeling. The fluorescence per cell, measured with a flow cytometer, is a measure of the total amount of the phagocytosed IgG. The results indicate that a rapid and quantitative method for the detection and measurement of phagocytosed CIC is possible using the flow cytometer.

Original language	English
Pages (from-to)	316-320
Journal	Cytometry
Volume	6
Issue number	4
DOIs	https://doi.org/10.1002/cyto.990060407
Publication status	Published - 1985

Keywords

Granulocytes
Phagocytosis
Circulating immune complexes
Flow cytometry

Access to Document

10.1002/cyto.990060407

ArticleFinal published version, 496 KB

Cite this

@article{175e34b6c24844a0894031389e687ba8,

title = "Flow cytometric determination of circulating immune complexes with the indirect granulocyte phagocytosis test",

abstract = "A method for the determination of circulating immune complexes (CIC) was adapted for flow cytometric analysis. Human granulocytes were used to phagocytose IgG-bearing CIC of serum from systemic lupus erythematosus (SLE) patients. A method for labeling the phagocytosed CIC with FITC-conjugated anti-human IgG was developed where the granulocytes remain in suspension during fixation and labeling. The fluorescence per cell, measured with a flow cytometer, is a measure of the total amount of the phagocytosed IgG. The results indicate that a rapid and quantitative method for the detection and measurement of phagocytosed CIC is possible using the flow cytometer.",

keywords = "Granulocytes, Phagocytosis, Circulating immune complexes, Flow cytometry",

author = "L.W.M.M. Terstappen and \{de Grooth\}, B.G. and G.M.J. Nolten and \{ten Napel\}, C.H.H. and \{van Berkel\}, W. and J. Greve",

year = "1985",

doi = "10.1002/cyto.990060407",

language = "English",

volume = "6",

pages = "316--320",

journal = "Cytometry",

issn = "0196-4763",

publisher = "Wiley",

number = "4",

}

TY - JOUR

T1 - Flow cytometric determination of circulating immune complexes with the indirect granulocyte phagocytosis test

AU - Terstappen, L.W.M.M.

AU - de Grooth, B.G.

AU - Nolten, G.M.J.

AU - ten Napel, C.H.H.

AU - van Berkel, W.

AU - Greve, J.

PY - 1985

Y1 - 1985

N2 - A method for the determination of circulating immune complexes (CIC) was adapted for flow cytometric analysis. Human granulocytes were used to phagocytose IgG-bearing CIC of serum from systemic lupus erythematosus (SLE) patients. A method for labeling the phagocytosed CIC with FITC-conjugated anti-human IgG was developed where the granulocytes remain in suspension during fixation and labeling. The fluorescence per cell, measured with a flow cytometer, is a measure of the total amount of the phagocytosed IgG. The results indicate that a rapid and quantitative method for the detection and measurement of phagocytosed CIC is possible using the flow cytometer.

AB - A method for the determination of circulating immune complexes (CIC) was adapted for flow cytometric analysis. Human granulocytes were used to phagocytose IgG-bearing CIC of serum from systemic lupus erythematosus (SLE) patients. A method for labeling the phagocytosed CIC with FITC-conjugated anti-human IgG was developed where the granulocytes remain in suspension during fixation and labeling. The fluorescence per cell, measured with a flow cytometer, is a measure of the total amount of the phagocytosed IgG. The results indicate that a rapid and quantitative method for the detection and measurement of phagocytosed CIC is possible using the flow cytometer.

KW - Granulocytes

KW - Phagocytosis

KW - Circulating immune complexes

KW - Flow cytometry

U2 - 10.1002/cyto.990060407

DO - 10.1002/cyto.990060407

M3 - Article

SN - 0196-4763

VL - 6

SP - 316

EP - 320

JO - Cytometry

JF - Cytometry

IS - 4

ER -

Flow cytometric determination of circulating immune complexes with the indirect granulocyte phagocytosis test

Abstract

Keywords

Access to Document

Fingerprint

Cite this