Flow-Free Microfluidic Device for Quantifying Chemotaxis in Spermatozoa

Johanna T.W. Berendsen*, Stella A. Kruit, Nihan Atak, Ellen Willink, Loes Segerink

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

30 Citations (Scopus)
101 Downloads (Pure)


Current male fertility diagnosis tests focus on assessing the quality of semen samples by studying the concentration, total volume, and motility of spermatozoa. However, other characteristics such as the chemotactic ability of a spermatozoon might influence the chance of fertilization. Here we describe a simple, easy to fabricate and handle, flow-free microfluidic chip to test the chemotactic response of spermatozoa made out of a hybrid hydrogel (8% gelatin/1% agarose). A chemotaxis experiment with 1 mu M progesterone was performed that significantly demonstrated that boar spermatozoa are attracted by a progesterone gradient.
Original languageEnglish
Pages (from-to)3302
Number of pages3306
JournalAnalytical chemistry
Issue number4
Publication statusPublished - 18 Feb 2020


  • UT-Hybrid-D
  • Animal derived food
  • Fluid dynamics
  • Diffusion
  • Amorphous materials
  • Hydrogels


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