Flow Through Gels as a Tool to Generate 3D Concentration Profiles in Hydrogel-Filled Devices

Joshua Loessberg-Zahl; Andries D. van der Meer; Albert  van den Berg; Jan C.T. Eijkel

Flow Through Gels as a Tool to Generate 3D Concentration Profiles in Hydrogel-Filled Devices

Applied Stem Cell Technologies

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

Laminar flow patterning is an iconic microfluidic technology used to locally deliver chemicals to specific regions on a two-dimensional surface with high spatial fidelity. Here we present a novel extension of this technology by flow patterning inside cured, three-dimensional (3D) hydrogels. We control the pressures at the inlets to shape the size and position of a region of high solute concentration in the main channel filled with a cured collagen 1 hydrogel. This method allows solutes to be delivered with 50 micron accuracy within the gel, as evidenced by control of concentration profiles of 40 kDa fluorescent dye.

Original language	English
Title of host publication	MicroTAS 2018
Subtitle of host publication	The 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences
Editors	Gwo-Bin Lee, Fan-Gang Tseng
Publisher	The Chemical and Biological Microsystems Society
Pages	2239-2241
Number of pages	3
ISBN (Electronic)	978-1-510-89757-1
ISBN (Print)	978-0-578-40530-8
Publication status	Published - 11 Nov 2018
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan Duration: 10 Nov 2018 → 15 Nov 2018 Conference number: 22 https://cbmsociety.org/conferences/microtas2018/

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018
Abbreviated title	MicroTAS 2018
Country/Territory	Taiwan
City	Kaohsiung
Period	10/11/18 → 15/11/18
Internet address	https://cbmsociety.org/conferences/microtas2018/

Keywords

2019 OA procedure

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Loessberg-Zahl2018flowFinal published version, 1.22 MBLicence: Taverne

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Loessberg-Zahl, J., van der Meer, A. D., van den Berg, A., & Eijkel, J. C. T. (2018). Flow Through Gels as a Tool to Generate 3D Concentration Profiles in Hydrogel-Filled Devices. In G.-B. Lee, & F.-G. T. (Eds.), MicroTAS 2018: The 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 2239-2241). The Chemical and Biological Microsystems Society.

Loessberg-Zahl, Joshua ; van der Meer, Andries D. ; van den Berg, Albert et al. / Flow Through Gels as a Tool to Generate 3D Concentration Profiles in Hydrogel-Filled Devices. MicroTAS 2018: The 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. editor / Gwo-Bin Lee ; Fan-Gang Tseng. The Chemical and Biological Microsystems Society, 2018. pp. 2239-2241

@inproceedings{ff5e54a74ffa4d61b384f2108b322348,

title = "Flow Through Gels as a Tool to Generate 3D Concentration Profiles in Hydrogel-Filled Devices",

abstract = "Laminar flow patterning is an iconic microfluidic technology used to locally deliver chemicals to specific regions on a two-dimensional surface with high spatial fidelity. Here we present a novel extension of this technology by flow patterning inside cured, three-dimensional (3D) hydrogels. We control the pressures at the inlets to shape the size and position of a region of high solute concentration in the main channel filled with a cured collagen 1 hydrogel. This method allows solutes to be delivered with 50 micron accuracy within the gel, as evidenced by control of concentration profiles of 40 kDa fluorescent dye.",

keywords = "2019 OA procedure",

author = "Joshua Loessberg-Zahl and \{van der Meer\}, \{Andries D.\} and \{van den Berg\}, Albert and Eijkel, \{Jan C.T.\}",

year = "2018",

month = nov,

day = "11",

language = "English",

isbn = "978-0-578-40530-8",

pages = "2239--2241",

editor = "Gwo-Bin Lee and \{Fan-Gang Tseng\}",

booktitle = "MicroTAS 2018",

publisher = "The Chemical and Biological Microsystems Society",

note = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018, MicroTAS 2018 ; Conference date: 10-11-2018 Through 15-11-2018",

url = "https://cbmsociety.org/conferences/microtas2018/",

}

Loessberg-Zahl, J , van der Meer, AD , van den Berg, A & Eijkel, JCT 2018, Flow Through Gels as a Tool to Generate 3D Concentration Profiles in Hydrogel-Filled Devices. in G-B Lee & F-GT (eds), MicroTAS 2018: The 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society, pp. 2239-2241, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018, Kaohsiung, Taiwan, 10/11/18.

Flow Through Gels as a Tool to Generate 3D Concentration Profiles in Hydrogel-Filled Devices. / Loessberg-Zahl, Joshua ; van der Meer, Andries D.; van den Berg, Albert et al.
MicroTAS 2018: The 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences. ed. / Gwo-Bin Lee; Fan-Gang Tseng. The Chemical and Biological Microsystems Society, 2018. p. 2239-2241.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Flow Through Gels as a Tool to Generate 3D Concentration Profiles in Hydrogel-Filled Devices

AU - Loessberg-Zahl, Joshua

AU - van der Meer, Andries D.

AU - van den Berg, Albert

AU - Eijkel, Jan C.T.

N1 - Conference code: 22

PY - 2018/11/11

Y1 - 2018/11/11

N2 - Laminar flow patterning is an iconic microfluidic technology used to locally deliver chemicals to specific regions on a two-dimensional surface with high spatial fidelity. Here we present a novel extension of this technology by flow patterning inside cured, three-dimensional (3D) hydrogels. We control the pressures at the inlets to shape the size and position of a region of high solute concentration in the main channel filled with a cured collagen 1 hydrogel. This method allows solutes to be delivered with 50 micron accuracy within the gel, as evidenced by control of concentration profiles of 40 kDa fluorescent dye.

AB - Laminar flow patterning is an iconic microfluidic technology used to locally deliver chemicals to specific regions on a two-dimensional surface with high spatial fidelity. Here we present a novel extension of this technology by flow patterning inside cured, three-dimensional (3D) hydrogels. We control the pressures at the inlets to shape the size and position of a region of high solute concentration in the main channel filled with a cured collagen 1 hydrogel. This method allows solutes to be delivered with 50 micron accuracy within the gel, as evidenced by control of concentration profiles of 40 kDa fluorescent dye.

KW - 2019 OA procedure

M3 - Conference contribution

SN - 978-0-578-40530-8

SP - 2239

EP - 2241

BT - MicroTAS 2018

A2 - Lee, Gwo-Bin

A2 - null, Fan-Gang Tseng

PB - The Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018

Y2 - 10 November 2018 through 15 November 2018

ER -

Flow Through Gels as a Tool to Generate 3D Concentration Profiles in Hydrogel-Filled Devices

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