Flow-Through Microbial Capture by Antibody-Coated Microsieves

A.T. Nguyen, R. van Doorn, J. Baggerman, Jos Marie Johannes Paulusse, M. Klerks, H. Zuilhof, C.J.M. van Rijn

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

A new flow-through method for rapid capture and detection of microorganisms is developed using optically-flat microengineered membranes. Selective and efficient capture of Salmonella is demonstrated with antibodies coated on membranes (microsieves) having a pore size much larger than the microorganism itself. The silicon-nitride membranes are first photochemically coated with 1,2-epoxy-9-decene yielding stable Si–C and N–C linkages. The resultant epoxide-terminated microsieves are subsequently biofunctionalized with anti-Salmonella antibodies. The capture efficiency of antibody-coated microsieves with different pore sizes (2.0–5.0 μm) is studied with Salmonella enterica enterica serotype Typhimurium suspensions (107 cfu mL–1). The antibody-coated microsieves capture 52% (2 μm microsieves), 30% (3.5 μm microsieves), and 12% (5 μm microsieves) of Salmonella from the suspension. The influence of flow rate (0.8–16 μL min–1 mm–2) on the capture efficiency of antibody-coated 3.5 μm microsieves is investigated. The capture efficiency increases from ≈30% to ≈70% when the flow-rate decreases from 16 to 0.8 μL min–1 mm–2. Antibody-coated 3.5 μm microsieves can capture Salmonella rapidly and directly from fresh milk suspension (capture 35% at concentration of 80 cfu mL–1). The use of antibody-coated microsieves as microbial selective capture devices is thus shown to be highly promising for the direct capture of microorganisms.
Original languageEnglish
Article number1400292
Number of pages9
JournalAdvanced materials interfaces
Volume2
Issue number3
DOIs
Publication statusPublished - 2015

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Antibodies
Salmonella
Microorganisms
Membranes
Pore size
Flow rate
Silicon nitride

Keywords

  • METIS-308064
  • IR-95035

Cite this

Nguyen, A. T., van Doorn, R., Baggerman, J., Paulusse, J. M. J., Klerks, M., Zuilhof, H., & van Rijn, C. J. M. (2015). Flow-Through Microbial Capture by Antibody-Coated Microsieves. Advanced materials interfaces, 2(3), [1400292]. https://doi.org/10.1002/admi.201400292
Nguyen, A.T. ; van Doorn, R. ; Baggerman, J. ; Paulusse, Jos Marie Johannes ; Klerks, M. ; Zuilhof, H. ; van Rijn, C.J.M. / Flow-Through Microbial Capture by Antibody-Coated Microsieves. In: Advanced materials interfaces. 2015 ; Vol. 2, No. 3.
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abstract = "A new flow-through method for rapid capture and detection of microorganisms is developed using optically-flat microengineered membranes. Selective and efficient capture of Salmonella is demonstrated with antibodies coated on membranes (microsieves) having a pore size much larger than the microorganism itself. The silicon-nitride membranes are first photochemically coated with 1,2-epoxy-9-decene yielding stable Si–C and N–C linkages. The resultant epoxide-terminated microsieves are subsequently biofunctionalized with anti-Salmonella antibodies. The capture efficiency of antibody-coated microsieves with different pore sizes (2.0–5.0 μm) is studied with Salmonella enterica enterica serotype Typhimurium suspensions (107 cfu mL–1). The antibody-coated microsieves capture 52{\%} (2 μm microsieves), 30{\%} (3.5 μm microsieves), and 12{\%} (5 μm microsieves) of Salmonella from the suspension. The influence of flow rate (0.8–16 μL min–1 mm–2) on the capture efficiency of antibody-coated 3.5 μm microsieves is investigated. The capture efficiency increases from ≈30{\%} to ≈70{\%} when the flow-rate decreases from 16 to 0.8 μL min–1 mm–2. Antibody-coated 3.5 μm microsieves can capture Salmonella rapidly and directly from fresh milk suspension (capture 35{\%} at concentration of 80 cfu mL–1). The use of antibody-coated microsieves as microbial selective capture devices is thus shown to be highly promising for the direct capture of microorganisms.",
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author = "A.T. Nguyen and {van Doorn}, R. and J. Baggerman and Paulusse, {Jos Marie Johannes} and M. Klerks and H. Zuilhof and {van Rijn}, C.J.M.",
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Nguyen, AT, van Doorn, R, Baggerman, J, Paulusse, JMJ, Klerks, M, Zuilhof, H & van Rijn, CJM 2015, 'Flow-Through Microbial Capture by Antibody-Coated Microsieves' Advanced materials interfaces, vol. 2, no. 3, 1400292. https://doi.org/10.1002/admi.201400292

Flow-Through Microbial Capture by Antibody-Coated Microsieves. / Nguyen, A.T.; van Doorn, R.; Baggerman, J.; Paulusse, Jos Marie Johannes; Klerks, M.; Zuilhof, H.; van Rijn, C.J.M.

In: Advanced materials interfaces, Vol. 2, No. 3, 1400292, 2015.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Flow-Through Microbial Capture by Antibody-Coated Microsieves

AU - Nguyen, A.T.

AU - van Doorn, R.

AU - Baggerman, J.

AU - Paulusse, Jos Marie Johannes

AU - Klerks, M.

AU - Zuilhof, H.

AU - van Rijn, C.J.M.

PY - 2015

Y1 - 2015

N2 - A new flow-through method for rapid capture and detection of microorganisms is developed using optically-flat microengineered membranes. Selective and efficient capture of Salmonella is demonstrated with antibodies coated on membranes (microsieves) having a pore size much larger than the microorganism itself. The silicon-nitride membranes are first photochemically coated with 1,2-epoxy-9-decene yielding stable Si–C and N–C linkages. The resultant epoxide-terminated microsieves are subsequently biofunctionalized with anti-Salmonella antibodies. The capture efficiency of antibody-coated microsieves with different pore sizes (2.0–5.0 μm) is studied with Salmonella enterica enterica serotype Typhimurium suspensions (107 cfu mL–1). The antibody-coated microsieves capture 52% (2 μm microsieves), 30% (3.5 μm microsieves), and 12% (5 μm microsieves) of Salmonella from the suspension. The influence of flow rate (0.8–16 μL min–1 mm–2) on the capture efficiency of antibody-coated 3.5 μm microsieves is investigated. The capture efficiency increases from ≈30% to ≈70% when the flow-rate decreases from 16 to 0.8 μL min–1 mm–2. Antibody-coated 3.5 μm microsieves can capture Salmonella rapidly and directly from fresh milk suspension (capture 35% at concentration of 80 cfu mL–1). The use of antibody-coated microsieves as microbial selective capture devices is thus shown to be highly promising for the direct capture of microorganisms.

AB - A new flow-through method for rapid capture and detection of microorganisms is developed using optically-flat microengineered membranes. Selective and efficient capture of Salmonella is demonstrated with antibodies coated on membranes (microsieves) having a pore size much larger than the microorganism itself. The silicon-nitride membranes are first photochemically coated with 1,2-epoxy-9-decene yielding stable Si–C and N–C linkages. The resultant epoxide-terminated microsieves are subsequently biofunctionalized with anti-Salmonella antibodies. The capture efficiency of antibody-coated microsieves with different pore sizes (2.0–5.0 μm) is studied with Salmonella enterica enterica serotype Typhimurium suspensions (107 cfu mL–1). The antibody-coated microsieves capture 52% (2 μm microsieves), 30% (3.5 μm microsieves), and 12% (5 μm microsieves) of Salmonella from the suspension. The influence of flow rate (0.8–16 μL min–1 mm–2) on the capture efficiency of antibody-coated 3.5 μm microsieves is investigated. The capture efficiency increases from ≈30% to ≈70% when the flow-rate decreases from 16 to 0.8 μL min–1 mm–2. Antibody-coated 3.5 μm microsieves can capture Salmonella rapidly and directly from fresh milk suspension (capture 35% at concentration of 80 cfu mL–1). The use of antibody-coated microsieves as microbial selective capture devices is thus shown to be highly promising for the direct capture of microorganisms.

KW - METIS-308064

KW - IR-95035

U2 - 10.1002/admi.201400292

DO - 10.1002/admi.201400292

M3 - Article

VL - 2

JO - Advanced materials interfaces

JF - Advanced materials interfaces

SN - 2196-7350

IS - 3

M1 - 1400292

ER -