Flow-through microfluidic chip for cell transfection by electropermeabilization

Ana Valero, Regina Lüttge, Jan William van Nieuwkasteele, Helene Andersson, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

1 Citation (Scopus)
15 Downloads (Pure)

Abstract

A new flow-through microfluidic chip is presented for the study of cell transfection by electroporation. A silicon-glass chip enables the immobilization of cells and real time monitoring of the electroporation process. Successful electroporation is shown by a propidium iodide (PI) assay and loading nanobeads.
Original languageEnglish
Title of host publicationMicroTAS 2005 Conference - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
EditorsK.F Jensen, J. Han, D.J. Harrison, J. Voldman
Place of PublicationSan Diego, CA, USA
PublisherTransducer Research Foundation, Inc.
Pages1395-1397
Number of pages3
ISBN (Print)9780974361116
Publication statusPublished - 9 Oct 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005 - Boston, United States
Duration: 9 Oct 200513 Oct 2005
Conference number: 9

Publication series

Name
PublisherTransducer Research Foundation, Inc.
Volume2

Conference

Conference9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2005
Abbreviated titleMicroTAS
CountryUnited States
CityBoston
Period9/10/0513/10/05

Keywords

  • electroporation
  • single cell manipulation
  • EWI-19990
  • Cell transfection
  • Flow-through
  • IR-52870
  • METIS-224699

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