Fluorescence in situ hybridization on human metaphase chromosomes detected by near‐field scanning optical microscopy

M.H.P. Moers, W.H.J. Kalle, A.G.T. Ruiter, J.C.A.G. Wiegant, A.K. Raap, J. Greve, B.G. de Grooth, N.F. van Hulst

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Abstract

Fluorescence in situ hybridization o­n human metaphase chromosomes is detected by near-field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments o­n a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1.77, p1-79 and the plasmid probe alpha-spectrin. The hybridization signals are very well resolved in the near-field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.
Original languageEnglish
Pages (from-to)40-45
Number of pages6
JournalJournal of microscopy
Volume182
Issue number1
DOIs
Publication statusPublished - 1996

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