Fluorescence in situ hybridization on human metaphase chromosomes detected by near‐field scanning optical microscopy

M.H.P. Moers; W.H.J. Kalle; A.G.T. Ruiter; J.C.A.G. Wiegant; A.K. Raap; J. Greve; B.G. de Grooth; N.F. van Hulst

doi:10.1111/j.1365-2818.1996.tb04795.x

Fluorescence in situ hybridization on human metaphase chromosomes detected by near‐field scanning optical microscopy

M.H.P. Moers, W.H.J. Kalle, A.G.T. Ruiter, J.C.A.G. Wiegant, A.K. Raap, J. Greve, B.G. de Grooth, N.F. van Hulst

Optical Sciences

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Abstract

Fluorescence in situ hybridization on human metaphase chromosomes is detected by near-field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments on a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1.77, p1-79 and the plasmid probe alpha-spectrin. The hybridization signals are very well resolved in the near-field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.

Original language	English
Pages (from-to)	40-45
Number of pages	6
Journal	Journal of microscopy
Volume	182
Issue number	1
DOIs	https://doi.org/10.1111/j.1365-2818.1996.tb04795.x
Publication status	Published - 1996

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10.1111/j.1365-2818.1996.tb04795.x

Moers1996fluorescenceFinal published version, 3.89 MB

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title = "Fluorescence in situ hybridization on human metaphase chromosomes detected by near‐field scanning optical microscopy",

abstract = "Fluorescence in situ hybridization on human metaphase chromosomes is detected by near-field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments on a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1.77, p1-79 and the plasmid probe alpha-spectrin. The hybridization signals are very well resolved in the near-field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.",

author = "M.H.P. Moers and W.H.J. Kalle and A.G.T. Ruiter and J.C.A.G. Wiegant and A.K. Raap and J. Greve and {de Grooth}, B.G. and {van Hulst}, N.F.",

year = "1996",

doi = "10.1111/j.1365-2818.1996.tb04795.x",

language = "English",

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journal = "Journal of microscopy",

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T1 - Fluorescence in situ hybridization on human metaphase chromosomes detected by near‐field scanning optical microscopy

AU - Moers, M.H.P.

AU - Kalle, W.H.J.

AU - Ruiter, A.G.T.

AU - Wiegant, J.C.A.G.

AU - Raap, A.K.

AU - Greve, J.

AU - de Grooth, B.G.

AU - van Hulst, N.F.

PY - 1996

Y1 - 1996

N2 - Fluorescence in situ hybridization on human metaphase chromosomes is detected by near-field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments on a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1.77, p1-79 and the plasmid probe alpha-spectrin. The hybridization signals are very well resolved in the near-field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.

AB - Fluorescence in situ hybridization on human metaphase chromosomes is detected by near-field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments on a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1.77, p1-79 and the plasmid probe alpha-spectrin. The hybridization signals are very well resolved in the near-field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.

U2 - 10.1111/j.1365-2818.1996.tb04795.x

DO - 10.1111/j.1365-2818.1996.tb04795.x

M3 - Article

SN - 0022-2720

VL - 182

SP - 40

EP - 45

JO - Journal of microscopy

JF - Journal of microscopy

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ER -