Fluorescence lifetime imaging is combined with atomic force microscopy in an integrated scanning microscope using a silicon-nitride probe. The time decay of fluorescence is measured at each image position with a resolution of 50 ps by time correlated single photon counting using a frequency doubled mode-locked Ti-Sapphire laser and fast electronics. Images of a mixture of fluorescence labelled latex spheres are presented where the lifetime contrast of the different spheres can be directly correlated to the topography as detected by force microscopy.
|Name||NATO ASI Series E|
|Publisher||Kluwer Academic Publishers|
|Workshop||NATO Advanced Research Workshop on Near Field Optics 1995|
|Period||11/09/95 → 15/09/95|