Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides

C. Dongre, R. Dekker, Hugo Hoekstra, R. Martinez-Vazquez, R. Osellame, R. Ramponi, G. Cerullo, R. van Weeghel, G.A.J. Besselink, H.H. van den Vlekkert, Markus Pollnau

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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Abstract

Femtosecond-laser-written optical waveguides were monolithically integrated into a commercial lab-on-a-chip to intersect a microfluidic channel. Laser excitation through these waveguides confines the excitation window to a width of 12 μm, enabling high-spatial-resolution monitoring of different fluorescent analytes, during their migration/separation in the microfluidic channel by capillary electrophoresis. Wavelength-selective monitoring of the on-chip separation of fluorescent dyes is implemented as a proof-of-principle. We envision well-controlled microfluidic plug formation, waveguide excitation, and a low limit of detection to enable monitoring of extremely small quantities with high spatial resolution.
Original languageEnglish
Title of host publicationProceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter
EditorsKerstin Worhoff, L. Agazzi, N. Ismail, X Leijtens
Place of PublicationEnschede, The Netherlands
PublisherIEEE/LEOS Benelux Chapter
Pages159-162
Number of pages4
ISBN (Print)978-90-365-2768-2
Publication statusPublished - 27 Nov 2008
Event13th Annual Symposium of the IEEE/LEOS Benelux Chapter 2008 - Enschede, Netherlands
Duration: 27 Nov 200828 Nov 2008
Conference number: 13

Conference

Conference13th Annual Symposium of the IEEE/LEOS Benelux Chapter 2008
CountryNetherlands
CityEnschede
Period27/11/0828/11/08

Fingerprint

Lab-on-a-chip
Electrophoresis
Microfluidics
Waveguides
Fluorescence
Monitoring
Capillary electrophoresis
Laser excitation
Optical waveguides
Ultrashort pulses
Fluorescent Dyes
Wavelength

Keywords

  • IOMS-SNS: SENSORS
  • EC Grant Agreement nr.: FP6/034562

Cite this

Dongre, C., Dekker, R., Hoekstra, H., Martinez-Vazquez, R., Osellame, R., Ramponi, R., ... Pollnau, M. (2008). Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides. In K. Worhoff, L. Agazzi, N. Ismail, & X. Leijtens (Eds.), Proceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter (pp. 159-162). Enschede, The Netherlands: IEEE/LEOS Benelux Chapter.
Dongre, C. ; Dekker, R. ; Hoekstra, Hugo ; Martinez-Vazquez, R. ; Osellame, R. ; Ramponi, R. ; Cerullo, G. ; van Weeghel, R. ; Besselink, G.A.J. ; van den Vlekkert, H.H. ; Pollnau, Markus. / Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides. Proceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter. editor / Kerstin Worhoff ; L. Agazzi ; N. Ismail ; X Leijtens. Enschede, The Netherlands : IEEE/LEOS Benelux Chapter, 2008. pp. 159-162
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abstract = "Femtosecond-laser-written optical waveguides were monolithically integrated into a commercial lab-on-a-chip to intersect a microfluidic channel. Laser excitation through these waveguides confines the excitation window to a width of 12 μm, enabling high-spatial-resolution monitoring of different fluorescent analytes, during their migration/separation in the microfluidic channel by capillary electrophoresis. Wavelength-selective monitoring of the on-chip separation of fluorescent dyes is implemented as a proof-of-principle. We envision well-controlled microfluidic plug formation, waveguide excitation, and a low limit of detection to enable monitoring of extremely small quantities with high spatial resolution.",
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author = "C. Dongre and R. Dekker and Hugo Hoekstra and R. Martinez-Vazquez and R. Osellame and R. Ramponi and G. Cerullo and {van Weeghel}, R. and G.A.J. Besselink and {van den Vlekkert}, H.H. and Markus Pollnau",
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Dongre, C, Dekker, R, Hoekstra, H, Martinez-Vazquez, R, Osellame, R, Ramponi, R, Cerullo, G, van Weeghel, R, Besselink, GAJ, van den Vlekkert, HH & Pollnau, M 2008, Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides. in K Worhoff, L Agazzi, N Ismail & X Leijtens (eds), Proceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter. IEEE/LEOS Benelux Chapter, Enschede, The Netherlands, pp. 159-162, 13th Annual Symposium of the IEEE/LEOS Benelux Chapter 2008, Enschede, Netherlands, 27/11/08.

Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides. / Dongre, C.; Dekker, R.; Hoekstra, Hugo; Martinez-Vazquez, R.; Osellame, R.; Ramponi, R.; Cerullo, G.; van Weeghel, R.; Besselink, G.A.J.; van den Vlekkert, H.H.; Pollnau, Markus.

Proceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter. ed. / Kerstin Worhoff; L. Agazzi; N. Ismail; X Leijtens. Enschede, The Netherlands : IEEE/LEOS Benelux Chapter, 2008. p. 159-162.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides

AU - Dongre, C.

AU - Dekker, R.

AU - Hoekstra, Hugo

AU - Martinez-Vazquez, R.

AU - Osellame, R.

AU - Ramponi, R.

AU - Cerullo, G.

AU - van Weeghel, R.

AU - Besselink, G.A.J.

AU - van den Vlekkert, H.H.

AU - Pollnau, Markus

PY - 2008/11/27

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N2 - Femtosecond-laser-written optical waveguides were monolithically integrated into a commercial lab-on-a-chip to intersect a microfluidic channel. Laser excitation through these waveguides confines the excitation window to a width of 12 μm, enabling high-spatial-resolution monitoring of different fluorescent analytes, during their migration/separation in the microfluidic channel by capillary electrophoresis. Wavelength-selective monitoring of the on-chip separation of fluorescent dyes is implemented as a proof-of-principle. We envision well-controlled microfluidic plug formation, waveguide excitation, and a low limit of detection to enable monitoring of extremely small quantities with high spatial resolution.

AB - Femtosecond-laser-written optical waveguides were monolithically integrated into a commercial lab-on-a-chip to intersect a microfluidic channel. Laser excitation through these waveguides confines the excitation window to a width of 12 μm, enabling high-spatial-resolution monitoring of different fluorescent analytes, during their migration/separation in the microfluidic channel by capillary electrophoresis. Wavelength-selective monitoring of the on-chip separation of fluorescent dyes is implemented as a proof-of-principle. We envision well-controlled microfluidic plug formation, waveguide excitation, and a low limit of detection to enable monitoring of extremely small quantities with high spatial resolution.

KW - IOMS-SNS: SENSORS

KW - EC Grant Agreement nr.: FP6/034562

M3 - Conference contribution

SN - 978-90-365-2768-2

SP - 159

EP - 162

BT - Proceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter

A2 - Worhoff, Kerstin

A2 - Agazzi, L.

A2 - Ismail, N.

A2 - Leijtens, X

PB - IEEE/LEOS Benelux Chapter

CY - Enschede, The Netherlands

ER -

Dongre C, Dekker R, Hoekstra H, Martinez-Vazquez R, Osellame R, Ramponi R et al. Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides. In Worhoff K, Agazzi L, Ismail N, Leijtens X, editors, Proceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter. Enschede, The Netherlands: IEEE/LEOS Benelux Chapter. 2008. p. 159-162