Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides

C. Dongre, R. Dekker, Hugo Hoekstra, R. Martinez-Vazquez, R. Osellame, R. Ramponi, G. Cerullo, R. van Weeghel, G.A.J. Besselink, H.H. van den Vlekkert, Markus Pollnau

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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    Abstract

    Femtosecond-laser-written optical waveguides were monolithically integrated into a commercial lab-on-a-chip to intersect a microfluidic channel. Laser excitation through these waveguides confines the excitation window to a width of 12 μm, enabling high-spatial-resolution monitoring of different fluorescent analytes, during their migration/separation in the microfluidic channel by capillary electrophoresis. Wavelength-selective monitoring of the on-chip separation of fluorescent dyes is implemented as a proof-of-principle. We envision well-controlled microfluidic plug formation, waveguide excitation, and a low limit of detection to enable monitoring of extremely small quantities with high spatial resolution.
    Original languageEnglish
    Title of host publicationProceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter
    EditorsKerstin Worhoff, L. Agazzi, N. Ismail, X Leijtens
    Place of PublicationEnschede, The Netherlands
    PublisherIEEE/LEOS Benelux Chapter
    Pages159-162
    Number of pages4
    ISBN (Print)978-90-365-2768-2
    Publication statusPublished - 27 Nov 2008
    Event13th Annual Symposium of the IEEE/LEOS Benelux Chapter 2008 - Enschede, Netherlands
    Duration: 27 Nov 200828 Nov 2008
    Conference number: 13

    Conference

    Conference13th Annual Symposium of the IEEE/LEOS Benelux Chapter 2008
    CountryNetherlands
    CityEnschede
    Period27/11/0828/11/08

    Keywords

    • IOMS-SNS: SENSORS
    • EC Grant Agreement nr.: FP6/034562

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  • Cite this

    Dongre, C., Dekker, R., Hoekstra, H., Martinez-Vazquez, R., Osellame, R., Ramponi, R., ... Pollnau, M. (2008). Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides. In K. Worhoff, L. Agazzi, N. Ismail, & X. Leijtens (Eds.), Proceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter (pp. 159-162). Enschede, The Netherlands: IEEE/LEOS Benelux Chapter.