Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides

C. Dongre; R. Dekker; Hugo Hoekstra; R. Martinez-Vazquez; R. Osellame; R. Ramponi; G. Cerullo; R. van Weeghel; G.A.J. Besselink; H.H. van den Vlekkert; Markus Pollnau

Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides

C. Dongre, R. Dekker, Hugo Hoekstra, R. Martinez-Vazquez, R. Osellame, R. Ramponi, G. Cerullo, R. van Weeghel, G.A.J. Besselink, H.H. van den Vlekkert, Markus Pollnau

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

Femtosecond-laser-written optical waveguides were monolithically integrated into a commercial lab-on-a-chip to intersect a microfluidic channel. Laser excitation through these waveguides confines the excitation window to a width of 12 μm, enabling high-spatial-resolution monitoring of different fluorescent analytes, during their migration/separation in the microfluidic channel by capillary electrophoresis. Wavelength-selective monitoring of the on-chip separation of fluorescent dyes is implemented as a proof-of-principle. We envision well-controlled microfluidic plug formation, waveguide excitation, and a low limit of detection to enable monitoring of extremely small quantities with high spatial resolution.

Original language	English
Title of host publication	Proceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter
Editors	Kerstin Worhoff, L. Agazzi, N. Ismail, X Leijtens
Place of Publication	Enschede, The Netherlands
Publisher	IEEE/LEOS Benelux Chapter
Pages	159-162
Number of pages	4
ISBN (Print)	978-90-365-2768-2
Publication status	Published - 27 Nov 2008
Event	13th Annual Symposium of the IEEE/LEOS Benelux Chapter 2008 - Enschede, Netherlands Duration: 27 Nov 2008 → 28 Nov 2008 Conference number: 13

Conference

Conference	13th Annual Symposium of the IEEE/LEOS Benelux Chapter 2008
Country	Netherlands
City	Enschede
Period	27/11/08 → 28/11/08

Keywords

IOMS-SNS: SENSORS
EC Grant Agreement nr.: FP6/034562

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Dongre, C., Dekker, R., Hoekstra, H., Martinez-Vazquez, R., Osellame, R., Ramponi, R., ... Pollnau, M. (2008). Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides. In K. Worhoff, L. Agazzi, N. Ismail, & X. Leijtens (Eds.), Proceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter (pp. 159-162). Enschede, The Netherlands: IEEE/LEOS Benelux Chapter.

Dongre, C. ; Dekker, R. ; Hoekstra, Hugo ; Martinez-Vazquez, R. ; Osellame, R. ; Ramponi, R. ; Cerullo, G. ; van Weeghel, R. ; Besselink, G.A.J. ; van den Vlekkert, H.H. ; Pollnau, Markus. / Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides. Proceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter. editor / Kerstin Worhoff ; L. Agazzi ; N. Ismail ; X Leijtens. Enschede, The Netherlands : IEEE/LEOS Benelux Chapter, 2008. pp. 159-162

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title = "Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides",

abstract = "Femtosecond-laser-written optical waveguides were monolithically integrated into a commercial lab-on-a-chip to intersect a microfluidic channel. Laser excitation through these waveguides confines the excitation window to a width of 12 μm, enabling high-spatial-resolution monitoring of different fluorescent analytes, during their migration/separation in the microfluidic channel by capillary electrophoresis. Wavelength-selective monitoring of the on-chip separation of fluorescent dyes is implemented as a proof-of-principle. We envision well-controlled microfluidic plug formation, waveguide excitation, and a low limit of detection to enable monitoring of extremely small quantities with high spatial resolution.",

keywords = "IOMS-SNS: SENSORS, EC Grant Agreement nr.: FP6/034562",

author = "C. Dongre and R. Dekker and Hugo Hoekstra and R. Martinez-Vazquez and R. Osellame and R. Ramponi and G. Cerullo and {van Weeghel}, R. and G.A.J. Besselink and {van den Vlekkert}, H.H. and Markus Pollnau",

year = "2008",

month = "11",

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language = "English",

isbn = "978-90-365-2768-2",

pages = "159--162",

editor = "Kerstin Worhoff and L. Agazzi and N. Ismail and X Leijtens",

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Dongre, C, Dekker, R, Hoekstra, H, Martinez-Vazquez, R, Osellame, R, Ramponi, R, Cerullo, G, van Weeghel, R, Besselink, GAJ, van den Vlekkert, HH & Pollnau, M 2008, Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides. in K Worhoff, L Agazzi, N Ismail & X Leijtens (eds), Proceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter. IEEE/LEOS Benelux Chapter, Enschede, The Netherlands, pp. 159-162, 13th Annual Symposium of the IEEE/LEOS Benelux Chapter 2008, Enschede, Netherlands, 27/11/08.

Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides. / Dongre, C.; Dekker, R.; Hoekstra, Hugo; Martinez-Vazquez, R.; Osellame, R.; Ramponi, R.; Cerullo, G.; van Weeghel, R.; Besselink, G.A.J.; van den Vlekkert, H.H.; Pollnau, Markus.

Proceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter. ed. / Kerstin Worhoff; L. Agazzi; N. Ismail; X Leijtens. Enschede, The Netherlands : IEEE/LEOS Benelux Chapter, 2008. p. 159-162.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides

AU - Dongre, C.

AU - Dekker, R.

AU - Hoekstra, Hugo

AU - Martinez-Vazquez, R.

AU - Osellame, R.

AU - Ramponi, R.

AU - Cerullo, G.

AU - van Weeghel, R.

AU - Besselink, G.A.J.

AU - van den Vlekkert, H.H.

AU - Pollnau, Markus

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N2 - Femtosecond-laser-written optical waveguides were monolithically integrated into a commercial lab-on-a-chip to intersect a microfluidic channel. Laser excitation through these waveguides confines the excitation window to a width of 12 μm, enabling high-spatial-resolution monitoring of different fluorescent analytes, during their migration/separation in the microfluidic channel by capillary electrophoresis. Wavelength-selective monitoring of the on-chip separation of fluorescent dyes is implemented as a proof-of-principle. We envision well-controlled microfluidic plug formation, waveguide excitation, and a low limit of detection to enable monitoring of extremely small quantities with high spatial resolution.

AB - Femtosecond-laser-written optical waveguides were monolithically integrated into a commercial lab-on-a-chip to intersect a microfluidic channel. Laser excitation through these waveguides confines the excitation window to a width of 12 μm, enabling high-spatial-resolution monitoring of different fluorescent analytes, during their migration/separation in the microfluidic channel by capillary electrophoresis. Wavelength-selective monitoring of the on-chip separation of fluorescent dyes is implemented as a proof-of-principle. We envision well-controlled microfluidic plug formation, waveguide excitation, and a low limit of detection to enable monitoring of extremely small quantities with high spatial resolution.

KW - IOMS-SNS: SENSORS

KW - EC Grant Agreement nr.: FP6/034562

M3 - Conference contribution

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SP - 159

EP - 162

BT - Proceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter

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A2 - Ismail, N.

A2 - Leijtens, X

PB - IEEE/LEOS Benelux Chapter

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Dongre C, Dekker R, Hoekstra H, Martinez-Vazquez R, Osellame R, Ramponi R et al. Fluorescence monitoring of capilarry electrophoresis separation in a lab-on-a-chip with monolithically integrated waveguides. In Worhoff K, Agazzi L, Ismail N, Leijtens X, editors, Proceedings of the 13th Annual Symposium of the IEEE LEOS Benelux Chapter. Enschede, The Netherlands: IEEE/LEOS Benelux Chapter. 2008. p. 159-162