Fluorescence monitoring of microchip capillary electrophoresis separation with monolithically integrated waveguides

C. Dongre, R. Dekker, Hugo Hoekstra, Markus Pollnau, R. Martinez-Vazquez, R. Osellame, G. Cerullo, R. Ramponi, R. van Weeghel, G.A.J. Besselink, H.H. van den Vlekkert

    Research output: Contribution to journalArticleAcademicpeer-review

    32 Citations (Scopus)

    Abstract

    Using femtosecond laser writing, optical waveguides were monolithically integrated into a commercial microfluidic lab-on-a-chip device, with the waveguides intersecting a microfluidic channel. Continuous-wave laser excitation through these optical waveguides confines the excitation window to a width of 12 μm, enabling high-resolution monitoring of the passage of different types of fluorescent analytes when migrating and being separated in the microfluidic channel by microchip capillary electrophoresis. Furthermore, we demonstrate on-chip-integrated waveguide excitation and detection of a biologically relevant species, fluorescently labeled DNA molecules, during microchip capillary electrophoresis. Well-controlled plug formation as required for on-chip integrated capillary electrophoresis separation of DNA molecules, and the combination of waveguide excitation and a low limit of detection, will enable monitoring of extremely small quantities with high spatial resolution.
    Original languageEnglish
    Pages (from-to)2503-2505
    Number of pages3
    JournalOptics letters
    Volume33
    Issue number21
    DOIs
    Publication statusPublished - 1 Nov 2008

    Keywords

    • IOMS-SNS: SENSORS
    • EC Grant Agreement nr.: FP6/034562

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