TY - JOUR
T1 - Fluorescent nanodiamonds encapsulated byCowpea Chlorotic Mottle Virus(CCMV) proteins for intracellular 3D-trajectory analysis
AU - Wu, Yingke
AU - Cao, Shuqin
AU - Alam, Md Noor A.
AU - Raabe, Marco
AU - Michel-Souzy, Sandra
AU - Wang, Zuyuan
AU - Wagner, Manfred
AU - Ermakova, Anna
AU - Cornelissen, Jeroen J.L.M.
AU - Weil, Tanja
N1 - Funding Information:
We thank Prof. Dr Theo Lasser for fruitful scientific discussions, Dr Siyuan Xiang for the measurement of circular dichroism spectroscopy and Pia Winterwerber for the atomic force microscopy measurement. Y. Wu thanks the China Scholarship Council (CSC) for offering a PhD scholarship. We are grateful for financial support from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)–Project number 316249678–SFB 1279 (C04) and ERC Consolidator Grant (Protcage #616907). Z. Wang acknowledges the financial support of an ERC Advanced Grant (SmartPhon #694977). Open Access funding provided by the Max Planck Society.
Funding Information:
We thank Prof. Dr Theo Lasser for fruitful scientific discussions, Dr Siyuan Xiang for the measurement of circular dichroism spectroscopy and Pia Winterwerber for the atomic force microscopy measurement. Y. Wu thanks the China Scholarship Council (CSC) for offering a PhD scholarship. We are grateful for financial support from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)-Project number 316249678-SFB 1279 (C04) and ERC Consolidator Grant (Protcage #616907). Z. Wang acknowledges the financial support of an ERC Advanced Grant (SmartPhon #694977). Open Access funding provided by the Max Planck Society.
Publisher Copyright:
© The Royal Society of Chemistry 2021.
PY - 2021/7/28
Y1 - 2021/7/28
N2 - Long-term tracking of nanoparticles to resolve intracellular structures and motions is essential to elucidate fundamental parameters as well as transport processes within living cells. Fluorescent nanodiamond (ND) emitters provide cell compatibility and very high photostability. However, high stability, biocompatibility, and cellular uptake of these fluorescent NDs under physiological conditions are required for intracellular applications. Herein, highly stable NDs encapsulated withCowpea chlorotic mottle viruscapsid proteins (ND-CP) are prepared. A thin capsid protein layer is obtained around the NDs, which imparts reactive groups and high colloidal stability, while retaining the opto-magnetic properties of the coated NDs as well as the secondary structure of CPs adsorbed on the surface of NDs. In addition, the ND-CP shows excellent biocompatibility bothin vitroandin vivo. Long-term 3D trajectories of the ND-CP with fine spatiotemporal resolutions are recorded; their intracellular motions are analyzed by different models, and the diffusion coefficients are calculated. The ND-CP with its brilliant optical properties and stability under physiological conditions provides us with a new tool to advance the understanding of cell biology,e.g., endocytosis, exocytosis, and active transport processes in living cells as well as intracellular dynamic parameters.
AB - Long-term tracking of nanoparticles to resolve intracellular structures and motions is essential to elucidate fundamental parameters as well as transport processes within living cells. Fluorescent nanodiamond (ND) emitters provide cell compatibility and very high photostability. However, high stability, biocompatibility, and cellular uptake of these fluorescent NDs under physiological conditions are required for intracellular applications. Herein, highly stable NDs encapsulated withCowpea chlorotic mottle viruscapsid proteins (ND-CP) are prepared. A thin capsid protein layer is obtained around the NDs, which imparts reactive groups and high colloidal stability, while retaining the opto-magnetic properties of the coated NDs as well as the secondary structure of CPs adsorbed on the surface of NDs. In addition, the ND-CP shows excellent biocompatibility bothin vitroandin vivo. Long-term 3D trajectories of the ND-CP with fine spatiotemporal resolutions are recorded; their intracellular motions are analyzed by different models, and the diffusion coefficients are calculated. The ND-CP with its brilliant optical properties and stability under physiological conditions provides us with a new tool to advance the understanding of cell biology,e.g., endocytosis, exocytosis, and active transport processes in living cells as well as intracellular dynamic parameters.
KW - UT-Hybrid-D
UR - http://www.scopus.com/inward/record.url?scp=85111103127&partnerID=8YFLogxK
U2 - 10.1039/d1tb00890k
DO - 10.1039/d1tb00890k
M3 - Article
C2 - 34184014
AN - SCOPUS:85111103127
SN - 2050-750X
VL - 9
SP - 5621
EP - 5627
JO - Journal of materials chemistry. B: materials for biology and medicine
JF - Journal of materials chemistry. B: materials for biology and medicine
IS - 28
ER -