Fluorescent nanodiamonds encapsulated byCowpea Chlorotic Mottle Virus(CCMV) proteins for intracellular 3D-trajectory analysis

Yingke Wu, Shuqin Cao, Md Noor A. Alam, Marco Raabe, Sandra Michel-Souzy, Zuyuan Wang, Manfred Wagner, Anna Ermakova, Jeroen J.L.M. Cornelissen*, Tanja Weil

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)
81 Downloads (Pure)


Long-term tracking of nanoparticles to resolve intracellular structures and motions is essential to elucidate fundamental parameters as well as transport processes within living cells. Fluorescent nanodiamond (ND) emitters provide cell compatibility and very high photostability. However, high stability, biocompatibility, and cellular uptake of these fluorescent NDs under physiological conditions are required for intracellular applications. Herein, highly stable NDs encapsulated withCowpea chlorotic mottle viruscapsid proteins (ND-CP) are prepared. A thin capsid protein layer is obtained around the NDs, which imparts reactive groups and high colloidal stability, while retaining the opto-magnetic properties of the coated NDs as well as the secondary structure of CPs adsorbed on the surface of NDs. In addition, the ND-CP shows excellent biocompatibility bothin vitroandin vivo. Long-term 3D trajectories of the ND-CP with fine spatiotemporal resolutions are recorded; their intracellular motions are analyzed by different models, and the diffusion coefficients are calculated. The ND-CP with its brilliant optical properties and stability under physiological conditions provides us with a new tool to advance the understanding of cell biology,e.g., endocytosis, exocytosis, and active transport processes in living cells as well as intracellular dynamic parameters.

Original languageEnglish
Pages (from-to)5621-5627
Number of pages7
JournalJournal of materials chemistry. B: materials for biology and medicine
Issue number28
Publication statusPublished - 28 Jul 2021


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