Fluorescently Labeled Branched Polymers and Thermal Responsive Nanoparticles for Live Cell Imaging

Di Zhou; Y. Ma; Andreas A. Poot; Pieter J. Dijkstra; Jan Feijen

doi:10.1002/mabi.201100254

Fluorescently Labeled Branched Polymers and Thermal Responsive Nanoparticles for Live Cell Imaging

Di Zhou, Y. Ma, Andreas A. Poot, Pieter J. Dijkstra, Jan Feijen

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Branched poly(methoxy-PEG acrylate) and thermally responsive poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) are synthesized by RAFT polymerization. After reduction, these polymers are fluorescently labeled by reacting the free thiol groups with N-(5-fluoresceinyl)maleimide. As shown by DLS, the labeled copolymer poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) forms nanoparticles at body temperature (37 °C) due to the presence of the thermosensitive poly(N-isopropylacrylamide). These materials were used as bioprobes for imaging HUVECs in vitro and chick embryo CAM in vivo. Both labeled polymer and nanoparticles are biocompatible and can be used as efficient fluorescent bioprobes

Original language	English
Pages (from-to)	465-474
Journal	Macromolecular bioscience
Volume	12
Issue number	4
DOIs	https://doi.org/10.1002/mabi.201100254
Publication status	Published - 2012

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title = "Fluorescently Labeled Branched Polymers and Thermal Responsive Nanoparticles for Live Cell Imaging",

abstract = "Branched poly(methoxy-PEG acrylate) and thermally responsive poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) are synthesized by RAFT polymerization. After reduction, these polymers are fluorescently labeled by reacting the free thiol groups with N-(5-fluoresceinyl)maleimide. As shown by DLS, the labeled copolymer poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) forms nanoparticles at body temperature (37 °C) due to the presence of the thermosensitive poly(N-isopropylacrylamide). These materials were used as bioprobes for imaging HUVECs in vitro and chick embryo CAM in vivo. Both labeled polymer and nanoparticles are biocompatible and can be used as efficient fluorescent bioprobes",

author = "Di Zhou and Y. Ma and Poot, {Andreas A.} and Dijkstra, {Pieter J.} and Jan Feijen",

year = "2012",

doi = "10.1002/mabi.201100254",

language = "English",

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pages = "465--474",

journal = "Macromolecular bioscience",

issn = "1616-5187",

publisher = "Wiley-VCH Verlag",

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T1 - Fluorescently Labeled Branched Polymers and Thermal Responsive Nanoparticles for Live Cell Imaging

AU - Zhou, Di

AU - Ma, Y.

AU - Poot, Andreas A.

AU - Dijkstra, Pieter J.

AU - Feijen, Jan

PY - 2012

Y1 - 2012

N2 - Branched poly(methoxy-PEG acrylate) and thermally responsive poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) are synthesized by RAFT polymerization. After reduction, these polymers are fluorescently labeled by reacting the free thiol groups with N-(5-fluoresceinyl)maleimide. As shown by DLS, the labeled copolymer poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) forms nanoparticles at body temperature (37 °C) due to the presence of the thermosensitive poly(N-isopropylacrylamide). These materials were used as bioprobes for imaging HUVECs in vitro and chick embryo CAM in vivo. Both labeled polymer and nanoparticles are biocompatible and can be used as efficient fluorescent bioprobes

AB - Branched poly(methoxy-PEG acrylate) and thermally responsive poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) are synthesized by RAFT polymerization. After reduction, these polymers are fluorescently labeled by reacting the free thiol groups with N-(5-fluoresceinyl)maleimide. As shown by DLS, the labeled copolymer poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) forms nanoparticles at body temperature (37 °C) due to the presence of the thermosensitive poly(N-isopropylacrylamide). These materials were used as bioprobes for imaging HUVECs in vitro and chick embryo CAM in vivo. Both labeled polymer and nanoparticles are biocompatible and can be used as efficient fluorescent bioprobes

U2 - 10.1002/mabi.201100254

DO - 10.1002/mabi.201100254

M3 - Article

SN - 1616-5187

VL - 12

SP - 465

EP - 474

JO - Macromolecular bioscience

JF - Macromolecular bioscience

IS - 4

ER -

Fluorescently Labeled Branched Polymers and Thermal Responsive Nanoparticles for Live Cell Imaging

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