Fluorescently Labeled Branched Polymers and Thermal Responsive Nanoparticles for Live Cell Imaging

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1 Citation (Scopus)

Abstract

Branched poly(methoxy-PEG acrylate) and thermally responsive poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) are synthesized by RAFT polymerization. After reduction, these polymers are fluorescently labeled by reacting the free thiol groups with N-(5-fluoresceinyl)maleimide. As shown by DLS, the labeled copolymer poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) forms nanoparticles at body temperature (37 °C) due to the presence of the thermosensitive poly(N-isopropylacrylamide). These materials were used as bioprobes for imaging HUVECs in vitro and chick embryo CAM in vivo. Both labeled polymer and nanoparticles are biocompatible and can be used as efficient fluorescent bioprobes
Original languageEnglish
Pages (from-to)465-474
JournalMacromolecular bioscience
Volume12
Issue number4
DOIs
Publication statusPublished - 2012

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Biosensing Techniques
Nanoparticles
Polyethylene glycols
Polymers
Hot Temperature
Imaging techniques
Computer aided manufacturing
Chick Embryo
Body Temperature
Sulfhydryl Compounds
Polymerization
Copolymers
Temperature
poly(methoxy-polyethylene glycol acrylate)-block-poly(N-isopropylacrylamide)
poly(methoxy-polyethylene glycol acrylate)
In Vitro Techniques
maleimide
poly-N-isopropylacrylamide

Cite this

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title = "Fluorescently Labeled Branched Polymers and Thermal Responsive Nanoparticles for Live Cell Imaging",
abstract = "Branched poly(methoxy-PEG acrylate) and thermally responsive poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) are synthesized by RAFT polymerization. After reduction, these polymers are fluorescently labeled by reacting the free thiol groups with N-(5-fluoresceinyl)maleimide. As shown by DLS, the labeled copolymer poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) forms nanoparticles at body temperature (37 °C) due to the presence of the thermosensitive poly(N-isopropylacrylamide). These materials were used as bioprobes for imaging HUVECs in vitro and chick embryo CAM in vivo. Both labeled polymer and nanoparticles are biocompatible and can be used as efficient fluorescent bioprobes",
author = "Di Zhou and Y. Ma and Poot, {Andreas A.} and Dijkstra, {Pieter J.} and Jan Feijen",
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journal = "Macromolecular bioscience",
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Fluorescently Labeled Branched Polymers and Thermal Responsive Nanoparticles for Live Cell Imaging. / Zhou, Di; Ma, Y.; Poot, Andreas A.; Dijkstra, Pieter J.; Feijen, Jan.

In: Macromolecular bioscience, Vol. 12, No. 4, 2012, p. 465-474.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Fluorescently Labeled Branched Polymers and Thermal Responsive Nanoparticles for Live Cell Imaging

AU - Zhou, Di

AU - Ma, Y.

AU - Poot, Andreas A.

AU - Dijkstra, Pieter J.

AU - Feijen, Jan

PY - 2012

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AB - Branched poly(methoxy-PEG acrylate) and thermally responsive poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) are synthesized by RAFT polymerization. After reduction, these polymers are fluorescently labeled by reacting the free thiol groups with N-(5-fluoresceinyl)maleimide. As shown by DLS, the labeled copolymer poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) forms nanoparticles at body temperature (37 °C) due to the presence of the thermosensitive poly(N-isopropylacrylamide). These materials were used as bioprobes for imaging HUVECs in vitro and chick embryo CAM in vivo. Both labeled polymer and nanoparticles are biocompatible and can be used as efficient fluorescent bioprobes

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