Focusing light through living tissue

I. M. Vellekoop, Christof M. Aegerter

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

45 Citations (Scopus)

Abstract

Tissues such as skin, fat or cuticle are non-transparent because inhomogeneities in the tissue scatter light. We demonstrate experimentally that light can be focused through turbid layers of living tissue, in spite of scattering. Our method is based on the fact that coherent light forms an interference pattern, even after hundreds of scattering events. By spatially shaping the wavefront of the incident laser beam, this interference pattern was modified to make the scattered light converge to a focus. In contrast to earlier experiments, where light was focused through solid objects, we focused light through living pupae of Drosophila melanogaster. We discuss a dynamic wavefront shaping algorithm that follows changes due to microscopic movements of scattering particles in real time. We relate the performance of the algorithm to the measured timescale of the changes in the speckle pattern and analyze our experiment in the light of Laser Doppler flowmetry. Applications in particle tracking, imaging, and optical manipulation are discussed.

Original languageEnglish
Title of host publicationOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV
Volume755430
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Tissue
Light
Scattering
Wavefronts
pupa
scattering
Drosophila
interference
Wave interference
coherent light
speckle patterns
fats
Pupa
Speckle
Laser-Doppler Flowmetry
Oils and fats
Optical Imaging
Laser beams
manipulators
Drosophila melanogaster

Keywords

  • Focusing
  • Interference
  • Turbid media
  • Wavefront shaping

Cite this

Vellekoop, I. M., & Aegerter, C. M. (2010). Focusing light through living tissue. In Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV (Vol. 755430). [755430] https://doi.org/10.1117/12.841159
Vellekoop, I. M. ; Aegerter, Christof M. / Focusing light through living tissue. Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV. Vol. 755430 2010.
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Vellekoop, IM & Aegerter, CM 2010, Focusing light through living tissue. in Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV. vol. 755430, 755430. https://doi.org/10.1117/12.841159

Focusing light through living tissue. / Vellekoop, I. M.; Aegerter, Christof M.

Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV. Vol. 755430 2010. 755430.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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AB - Tissues such as skin, fat or cuticle are non-transparent because inhomogeneities in the tissue scatter light. We demonstrate experimentally that light can be focused through turbid layers of living tissue, in spite of scattering. Our method is based on the fact that coherent light forms an interference pattern, even after hundreds of scattering events. By spatially shaping the wavefront of the incident laser beam, this interference pattern was modified to make the scattered light converge to a focus. In contrast to earlier experiments, where light was focused through solid objects, we focused light through living pupae of Drosophila melanogaster. We discuss a dynamic wavefront shaping algorithm that follows changes due to microscopic movements of scattering particles in real time. We relate the performance of the algorithm to the measured timescale of the changes in the speckle pattern and analyze our experiment in the light of Laser Doppler flowmetry. Applications in particle tracking, imaging, and optical manipulation are discussed.

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Vellekoop IM, Aegerter CM. Focusing light through living tissue. In Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV. Vol. 755430. 2010. 755430 https://doi.org/10.1117/12.841159